{"id":10959,"date":"2026-11-12T08:53:22","date_gmt":"2026-11-12T07:53:22","guid":{"rendered":"https:\/\/seamlessbio.de\/?page_id=10959"},"modified":"2026-11-12T08:56:02","modified_gmt":"2026-11-12T07:56:02","slug":"serumfreie-tierfreie-zellkultur","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/serum-free-animal-free-cell-culture\/","title":{"rendered":"Serumfreie und tierfreie Zellkultur"},"content":{"rendered":"\n\n

Serum-Free and Animal-Free Cell Culture<\/h1>
At a glance<\/span>

Most labs move away from FBS in two steps. First they replace the bovine serum with a human or recombinant supplement. Later, where the process allows it, they switch to a fully defined medium. We supply both from Germany: recombinant human albumin, human platelet lysate, human AB serum and serum-free media for CHO, HEK293, MSC and stem cells.<\/p><\/div>

Serum-free, xeno-free, animal-free: what the terms mean<\/h2>

The terms are often mixed up. For a raw material file the difference matters, so here is how we use them.<\/p>
Term<\/th>Meaning<\/th>Our products<\/th><\/tr><\/thead>
Serum-free<\/td>The medium contains no serum. It may still contain purified proteins such as albumin or transferrin.<\/td>Serum-free media; rHSA as albumin source<\/td><\/tr>
Xeno-free<\/td>No material from non-human species. Human-derived components are allowed.<\/td>hPL, human AB serum, rHSA<\/td><\/tr>
Animal component-free (ACF)<\/td>No raw materials of animal origin anywhere in the product.<\/td>rHSA Premium and Economy (expressed in rice), animal-free media<\/td><\/tr>
Chemically defined<\/td>Every component and its concentration is known.<\/td>SimplaceCDX, CelliMax\u00ae CHO media<\/td><\/tr><\/tbody><\/table>

Supplements that replace FBS<\/h2>

These go into the medium you already use. That makes them the quickest way to drop bovine serum.<\/p>
Product<\/th>Source and key data<\/th>Typical use<\/th><\/tr><\/thead>
rHSA Premium<\/a><\/td>Recombinant human albumin, expressed in rice endosperm. \u226599 % purity (SDS-PAGE), endotoxin <0.125 EU\/mg, lyophilised powder.<\/td>Albumin in serum-free and xeno-free media for CHO, HEK293 and Vero cells; donor-free supplement for CAR-T, NK and MSC culture; freezing media.<\/td><\/tr>
rHSA Economy<\/a><\/td>Recombinant human albumin, expressed in rice endosperm. 90\u201399 % purity, endotoxin <5 EU\/mg.<\/td>IVD reagents, blocking, protein stabilisation and method development. For cell culture we recommend Premium.<\/td><\/tr>
Human platelet lysate (hPL)<\/a><\/td>From EU platelet units of healthy donors. Research, GMP and GMP-clinical (virus-inactivated) grades. GMP grade is fibrinogen-depleted and heparin-free; 50, 100 and 500 mL.<\/td>MSC expansion and primary cells at 1\u201310 % (v\/v) in MEM \u03b1 or DMEM; cell therapy process development.<\/td><\/tr>
Human AB serum<\/a><\/td>Blood group AB, no anti-A or anti-B antibodies. Male or mixed donors, off-the-clot from male donors; EU or US donor centres. Native, heat inactivated or gamma irradiated.<\/td>T cell and CAR-T culture, NK cell expansion, MSC isolation and expansion.<\/td><\/tr><\/tbody><\/table>

Serum-free media by cell type<\/h2>
Cell type<\/th>Recommended medium<\/th>Grade<\/th><\/tr><\/thead>
CHO<\/a><\/td>CelliMax\u00ae CHO basal media (MetaCHO, Versa) and feeds (FGa, FGa plus, FGb)<\/td>GMP grade<\/td><\/tr>
HEK293 \u2013 research<\/a><\/td>SimplaceX (serum-free) and SimplaceCDX (chemically defined) for HEK293T<\/td>Research grade<\/td><\/tr>
HEK293 \u2013 viral vectors<\/a><\/td>CelliMax\u00ae Trans293 and TransV-293 for AAV and lentiviral vectors<\/td>GMP grade<\/td><\/tr>
MSC<\/a><\/td>CelliMax\u00ae MSC medium for bone marrow, adipose and umbilical cord MSC, xeno-free<\/td>GMP grade<\/td><\/tr>
iPSC and hESC<\/a><\/td>CelliMax\u00ae hPSC medium, feeder-free and xeno-free<\/td>GMP grade<\/td><\/tr>
Naive iPSC<\/a><\/td>NaivaCore, based on the HENSM formulation<\/td>Research grade<\/td><\/tr>
Organoids<\/a><\/td>OrganoMedium for organoid expansion<\/td>On request<\/td><\/tr>
Other cell lines<\/a><\/td>Custom media, formulated for your cell line and process<\/td>On request<\/td><\/tr><\/tbody><\/table>

Where to start<\/h2>

You grow MSC or primary cells in FBS<\/h3>

Start with hPL at 1\u201310 % (v\/v) in your current basal medium. The fibrinogen-depleted GMP grade needs no heparin. The rest of the protocol usually stays the same.<\/p>

You expand T cells, CAR-T or NK cells<\/h3>

Human AB serum is the usual human supplement here. Where the material must be donor-free, rHSA Premium takes over the albumin function.<\/p>

You build a defined production process<\/h3>

For CHO and HEK293 production, use a serum-free or chemically defined medium. Add rHSA Premium where your process calls for albumin.<\/p>

How to order<\/h2>

Free test sample<\/h3>

Test the material in your own cells before you order.<\/p><\/div>

Lot reservation<\/h3>

We hold your lot of serum, hPL or albumin for up to 36 months, without prepayment.<\/p><\/div>

Delivery from Germany<\/h3>

Cold-chain shipping to the EU, UK and Switzerland.<\/p><\/div><\/div>