{"id":1416,"date":"2026-06-21T20:32:17","date_gmt":"2026-06-21T19:32:17","guid":{"rendered":"https:\/\/wheat-quail-856400.hostingersite.com\/?page_id=1416"},"modified":"2026-11-12T08:03:05","modified_gmt":"2026-11-12T07:03:05","slug":"mrp4-vesikelkit","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/human-mrp4-vesicle-kit\/","title":{"rendered":"MRP4-Vesikel-Kit"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"1416\" class=\"elementor elementor-1416\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-200bc31 e-con e-atomic-element e-flexbox-base e-default-div e-29869f3\" data-id=\"200bc31\" data-element_type=\"e-flexbox\" data-e-type=\"e-flexbox\" data-interaction-id=\"200bc31\">\n    \t\t<div class=\"elementor-element elementor-element-dcca3f1 elementor-widget elementor-widget-html\" data-id=\"dcca3f1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t\t<section class=\"sb-vk-mrp4-page\">\n  <div class=\"sb-vk-mrp4-container\">\n\n    <div class=\"sb-vk-mrp4-breadcrumbs\">\n      <a href=\"https:\/\/seamlessbio.de\/de\/\">Startseite<\/a>\n      <span>\/<\/span>\n      <a href=\"https:\/\/seamlessbio.de\/de\/dmpk-transporter-bausatz\/\">DMPK-Transporter-Kit<\/a>\n      <span>\/<\/span>\n      <strong>MRP4-Vesikel-Kit<\/strong>\n    <\/div>\n\n    <div class=\"sb-vk-mrp4-hero\">\n      <div class=\"sb-vk-mrp4-hero-text\">\n        <span>SeamlessBio \u2013 Cell4Pharma DMPK-Assays<\/span>\n        <h1>MRP4-Vesikel-Kit f\u00fcr den Menschen<\/h1>\n        <p>\n          Aus HEK293-Zellen gewonnene Inside-Out-Membranvesikel f\u00fcr das Screening zur MRP4-Hemmung und die Risikobewertung von Arzneimittelwechselwirkungen (DDI) gem\u00e4\u00df den Testempfehlungen der FDA und der EMA.\n        <\/p>\n        <div class=\"sb-vk-mrp4-herobtns\">\n          <a href=\"\/about-us\/contact-imprint\/\" class=\"sb-vk-mrp4-btn\">Informationen anfordern<\/a>\n          <a href=\"https:\/\/seamlessbio.de\/de\/produkt\/mrp4-vesikelkit-abcc4-hek293\/\" class=\"sb-vk-mrp4-btn-outline\">Im Shop ansehen<\/a>\n        <\/div>\n      <\/div>\n      <div class=\"sb-vk-mrp4-hero-image\">\n        <img fetchpriority=\"high\" decoding=\"async\" width=\"1200\" height=\"900\" loading=\"eager\" src=\"https:\/\/seamlessbio.de\/wp-content\/uploads\/2026\/11\/cell4pharma-mrp4-vesicle-kit.jpg\" alt=\"Human MRP4 vesicle kit by Cell4Pharma\"\n          onerror=\"this.style.display='none'; this.nextElementSibling.style.display='flex';\">\n        <div class=\"sb-vk-mrp4-img-fallback\" style=\"display:none;\">Platzhalter f\u00fcr Produktbild<\/div>\n      <\/div>\n    <\/div>\n<!-- BUY BOX (SEO: links to buy pages) -->\n<div class=\"sb-buy-box\" style=\"border:1px solid #cfe9e6;background:#f4fbfa;border-radius:12px;padding:22px 26px;margin:28px auto;max-width:1200px;box-sizing:border-box\">\n<h2 style=\"font-size:22px;line-height:1.3;margin:0 0 6px\">ABC-Transporter-Vesikelkits kaufen (aus HEK293)<\/h2>\n<p style=\"margin:0 0 14px\">Angebot f\u00fcr P-gp-, BCRP-, BSEP- und MRP-Vesikelkits f\u00fcr In-vitro-DDI-Studien anfragen.<\/p>\n<ul style=\"margin:0;padding-left:20px;columns:2 300px;column-gap:32px\">\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/mrp4-vesikelkit-abcc4-hek293\/\" style=\"font-weight:700\">MRP4-Vesikelkit (ABCC4)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/p-gp-vesikelkit-mdr1-hek293\/\">P-gp-Vesikelkit (MDR1\/ABCB1)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/bcrp-vesikelkit-abcg2-hek293\/\">BCRP-Vesikelkit (ABCG2)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/bsep-vesikelkit-abcb11-hek293\/\">BSEP-Vesikelkit (ABCB11)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/mrp1-vesikelkit-abcc1-hek293\/\">MRP1-Vesikelkit (ABCC1)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/mrp2-vesikelkit-abcc2-hek293\/\">MRP2-Vesikelkit (ABCC2)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/mrp3-vesikelkit-abcc3-hek293\/\">MRP3-Vesikelkit (ABCC3)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/mrp5-vesikelkit-abcc5-hek293\/\">MRP5-Vesikelkit (ABCC5)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/mrp8-vesikelkit-abcc11-hek293\/\">MRP8-Vesikelkit (ABCC11)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/p-gp-vesikelkit-fluopgp-substrat\/\">P-gp-Vesikelkit mit FluoPgp-Substrat<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/kontroll-vesikelkit-hek293\/\">Kontroll-Vesikelkit (Negativkontrolle)<\/a><\/li>\n<\/ul>\n<p style=\"margin:14px 0 0\"><a href=\"https:\/\/seamlessbio.de\/de\/request-quote\/\" style=\"font-weight:600\">Angebot anfordern \u2192<\/a><\/p>\n<\/div>\n<!-- \/BUY BOX -->\n\n\n    <div class=\"sb-vk-mrp4-grid\">\n\n      <div class=\"sb-vk-mrp4-card\">\n        <span>01<\/span>\n        <h3>Bedeutung von Nukleotidanaloga<\/h3>\n        <p>\n          Besonders relevant f\u00fcr antivirale Nukleotidanaloga und Chemotherapeutika, sowohl als Substrat als auch als Inhibitor.\n        <\/p>\n      <\/div>\n\n      <div class=\"sb-vk-mrp4-card\">\n        <span>02<\/span>\n        <h3>Inside-Out-Vesikel<\/h3>\n        <p>\n          Von HEK293-Zellen stammende Membranvesikel mit nachgewiesener MRP4-Expression und \u201eInside-out\u201c-Orientierung f\u00fcr zuverl\u00e4ssige, reproduzierbare Transportdaten.\n        <\/p>\n      <\/div>\n\n      <div class=\"sb-vk-mrp4-card\">\n        <span>03<\/span>\n        <h3>100 Reaktionen pro Kit<\/h3>\n        <p>\n          Jedes Kit ist f\u00fcr 100 Reaktionen im 96-Well-Platten-Format ausgelegt\nund eignet sich zur IC50-Bestimmung sowie f\u00fcr das Screening mit einer einzigen Konzentration\n.\n        <\/p>\n      <\/div>\n\n      <div class=\"sb-vk-mrp4-card\">\n        <span>04<\/span>\n        <h3>Trockeneisversand<\/h3>\n        <p>\n          Wurde bei \u221280 \u00b0C auf Trockeneis tiefgefroren versandt, um die Integrit\u00e4t der Vesikel\nund die Transportaktivit\u00e4t bei Ankunft zu gew\u00e4hrleisten.\n        <\/p>\n      <\/div>\n\n    <\/div>\n\n    <div class=\"sb-vk-mrp4-content\">\n\n      <div class=\"sb-vk-mrp4-text\">\n        <h2>Warum MRP4-Tests in DMPK-Programmen wichtig sind<\/h2>\n        <p>\n          MRP4 (ABCC4) ist ein weit verbreiteter Transporter mit Substratspezifit\u00e4t f\u00fcr zyklische Nukleotide, Gallens\u00e4uren und eine Reihe von Nukleotid-Analoga.\n        <\/p>\n        <p>\n          Der vesikelbasierte Assay nutzt zyklisches AMP oder Estronsulfat als Sondersubstrat, um den MRP4-vermittelten Transport in Inside-Out-Membranvesikel in Anwesenheit und Abwesenheit von Testverbindungen zu quantifizieren, wodurch sich die Hemmwirksamkeit und die IC50-Werte bestimmen lassen.\n        <\/p>\n        <p>\n          Die Hemmung von MRP4 ist sowohl f\u00fcr den Gallens\u00e4ureausfluss aus der Leber (neben MRP3 als kompensatorischem Weg) als auch f\u00fcr den Stoffwechsel von antiviralen Nukleotidanaloga und Chemotherapeutika relevant, die direkte MRP4-Substrate sind. MRP4 ist einer von mehreren Transportern, die untersucht werden, wenn eine Wirkstoffkandidatin selbst ein Nukleotidanalogon ist, da MRP4 sowohl den eigenen Ausstrom vermitteln als auch den Transport endogener Gallens\u00e4uren hemmen kann.\n        <\/p>\n      <\/div>\n\n      <div class=\"sb-vk-mrp4-list\">\n        <h3>Typische Anwendungsbereiche<\/h3>\n        <ul>\n          <li>Risikobewertung bei arzneimittelinduzierten Lebersch\u00e4den (DILI)<\/li>\n          <li>Studien zu Arzneimittelwechselwirkungen (DDI) gem\u00e4\u00df den Leitlinien der FDA\/EMA<\/li>\n          <li>Hepatotoxizit\u00e4ts-Screening bei der Leitstoffoptimierung<\/li>\n          <li>Bestimmung des IC50-Werts f\u00fcr MRP4-Inhibitoren<\/li>\n          <li>Regulatorische Transporter-Panels (BSEP, BCRP, P-gp, MRP1\u20135, MRP8)<\/li>\n          <li>Forschung im akademischen und pharmazeutischen Bereich zu DMPK<\/li>\n        <\/ul>\n      <\/div>\n\n    <\/div>\n\n    <div class=\"sb-vk-mrp4-details\">\n      <div>\n        <h3>Produktinformationen<\/h3>\n        <ul>\n          <li>Produkttyp: MRP4-Vesikel-Kit (menschlich)<\/li>\n          <li>Quelle: Aus HEK293-Zellen gewonnene Inside-Out-Membranvesikel<\/li>\n          <li>Kit-Gr\u00f6\u00dfe: 100 Reaktionen (96-Well-Plattenformat)<\/li>\n          <li>Substrat: zyklisches AMP oder Estronsulfat<\/li>\n          <li>Lagerung: \u221280 \u00b0C<\/li>\n          <li>Versand: Trockeneis<\/li>\n        <\/ul>\n      <\/div>\n\n      <div>\n        <h3>Auf Anfrage erh\u00e4ltlich<\/h3>\n        <ul>\n          <li>Analysezertifikat (CoA)<\/li>\n          <li>Technisches Datenblatt<\/li>\n          <li>Unterst\u00fctzung bei der Erstellung von Assay-Protokollen<\/li>\n          <li>Vollst\u00e4ndiges Zitat zum ABC-Transporter-Panel<\/li>\n          <li>Optionen f\u00fcr Gro\u00dfbestellungen<\/li>\n        <\/ul>\n      <\/div>\n    <\/div>\n\n    <!-- SB-KITDATA -->\n<section class=\"sb-pd sb-kitdata\" style=\"max-width:1100px;margin:32px auto 8px;padding:0 24px;box-sizing:border-box\"><h2>Kitdetails<\/h2><table><thead><tr><th>Parameter<\/th><th>Wert<\/th><\/tr><\/thead><tbody><tr><td>Katalognummer<\/td><td>C4PVK004<\/td><\/tr><tr><td>Proteinkonzentration der Vesikel<\/td><td>1.5 mg\/mL<\/td><\/tr><tr><td>Vesikelvolumen<\/td><td>4 \u00d7 500 \u00b5L<\/td><\/tr><tr><td>Assays pro Kit<\/td><td>100<\/td><\/tr><tr><td>Vesikelpuffer<\/td><td>250 mM Saccharose, 10 mM Tris-HEPES, pH 7,4<\/td><\/tr><tr><td>Probesubstrat<\/td><td>DHEAS<\/td><\/tr><tr><td>ATP\/AMP-Transportverh\u00e4ltnis<\/td><td>> 5<\/td><\/tr><tr><td>Lagerung<\/td><td>Vesikel bei \u221280 \u00b0C; Puffer bei \u221220 \u00b0C<\/td><\/tr><tr><td>Haltbarkeit<\/td><td>Zwei Jahre ab Versand<\/td><\/tr><tr><td>Ebenfalls im Kit<\/td><td>Stopp-\/Waschpuffer (2\u00d7), Assaypuffer (2\u00d7), MgCl\u2082 500 mM, ATP 100 mM, AMP 100 mM<\/td><\/tr><\/tbody><\/table><\/section>\n<!-- \/SB-KITDATA -->\n<!-- SB-PUBS -->\n<section class=\"sb-pd sb-pubs\" style=\"max-width:1100px;margin:32px auto 8px;padding:0 24px;box-sizing:border-box\"><h2>Ausgew\u00e4hlte Publikationen<\/h2><p>Begutachtete Studien mit Daten aus Vesikeltransportassays, aus der Publikationsliste von Cell4Pharma.<\/p><ul><li>Koenderink JB et al. (2020). Human multidrug resistance protein 4 (MRP4) is a cellular efflux transporter for paracetamol glutathione and cysteine conjugates. <em>Arch Toxicol<\/em>. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32472168\/\" target=\"_blank\" rel=\"noopener\">PubMed 32472168<\/a><\/li><li>Weigand KM et al. (2019). Uremic solutes modulate hepatic bile acid handling and induce mitochondrial toxicity. <em>Toxicol In Vitro<\/em>. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/30639138\/\" target=\"_blank\" rel=\"noopener\">PubMed 30639138<\/a><\/li><li>J\u00e4rvinen E et al. (2019). Efflux transport of nicotine, cotinine and trans-3'-hydroxycotinine glucuronides by human hepatic transporters. <em>Basic Clin Pharmacol Toxicol<\/em>. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31237077\/\" target=\"_blank\" rel=\"noopener\">PubMed 31237077<\/a><\/li><li>Lempers VJ et al. (2016). Inhibitory Potential of Antifungal Drugs on ATP-Binding Cassette Transporters P-Glycoprotein, MRP1 to MRP5, BCRP, and BSEP. <em>Antimicrob Agents Chemother<\/em>. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27001813\/\" target=\"_blank\" rel=\"noopener\">PubMed 27001813<\/a><\/li><li>El-Sheikh AA et al. (2014). Renal glucuronidation and multidrug resistance protein 2-\/ multidrug resistance protein 4-mediated efflux of mycophenolic acid: interaction with cyclosporine and tacrolimus. <em>Transl Res<\/em>. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/24486136\/\" target=\"_blank\" rel=\"noopener\">PubMed 24486136<\/a><\/li><li>Rijpma SR et al. (2014). Atovaquone and quinine anti-malarials inhibit ATP binding cassette transporter activity. <em>Malar J<\/em>. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25218605\/\" target=\"_blank\" rel=\"noopener\">PubMed 25218605<\/a><\/li><\/ul><\/section>\n<!-- \/SB-PUBS -->\n<div class=\"sb-vk-mrp4-related\">\n      <h3>Verwandte Transporter-Kits<\/h3>\n      <div class=\"sb-vk-mrp4-related-grid\">\n        <a href=\"https:\/\/seamlessbio.de\/de\/dmpk-transporter-bausatz\/abc-transporter-vesikelkits\/mrp3-vesikelkit\/\">MRP3-Vesikel-Kit<\/a>\n        <a href=\"https:\/\/seamlessbio.de\/de\/dmpk-transporter-bausatz\/abc-transporter-vesikelkits\/mrp5-vesikelkit\/\">MRP5-Vesikel-Kit<\/a>\n        <a href=\"https:\/\/seamlessbio.de\/de\/dmpk-transporter-bausatz\/abc-transporter-vesikelkits\/bsep-vesikelkit\/\">BSEP-Vesikel-Kit<\/a>\n        <a href=\"https:\/\/seamlessbio.de\/de\/dmpk-transporter-bausatz\/\">Alle Vesikel-Kits \u2013 \u00dcbersicht<\/a>\n      <\/div>\n    <\/div>\n\n    <div class=\"sb-vk-mrp4-cta\">\n      <h2>Ben\u00f6tigen Sie ein komplettes ABC-Transporter-Panel?<\/h2>\n      <p>\n        Wenden Sie sich an unser Team, um Informationen zu den Preisen f\u00fcr BSEP-, BCRP-, P-gp- und MRP-Kits,\nzur technischen Dokumentation sowie zur Unterst\u00fctzung bei der Assay-Durchf\u00fchrung f\u00fcr Ihre DMPK-Studie zu erhalten.\n      <\/p>\n      <a href=\"\/about-us\/contact-imprint\/\">Kontakt<\/a>\n    <\/div>\n\n  <\/div>\n<\/section>\n\n<style>\n.sb-vk-mrp4-page,\n.sb-vk-mrp4-page *,\n.sb-vk-mrp4-page *::before,\n.sb-vk-mrp4-page *::after {\n  box-sizing: border-box;\n}\n\n.sb-vk-mrp4-page {\n  background: #ffffff;\n  padding: 70px 40px 90px;\n  font-family: Arial, sans-serif;\n  color: #202020;\n}\n\n.sb-vk-mrp4-container {\n  max-width: 1400px;\n  width: 100%;\n  margin: 0 auto;\n}\n\n.sb-vk-mrp4-breadcrumbs {\n  display: flex;\n  flex-wrap: wrap;\n  gap: 8px;\n  margin: 0 0 24px;\n  color: #777;\n  font-size: 14px;\n  word-break: 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