{"id":1416,"date":"2026-06-21T20:32:17","date_gmt":"2026-06-21T19:32:17","guid":{"rendered":"http:\/\/seamlessbio.de\/?page_id=1416"},"modified":"2026-06-21T20:38:29","modified_gmt":"2026-06-21T19:38:29","slug":"human-mrp4-vesicle-kit","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/human-mrp4-vesicle-kit\/","title":{"rendered":"MRP4-Vesikel-Kit"},"content":{"rendered":"
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\n Startseite<\/a>\n \/<\/span>\n DMPK Transporter Kit<\/a>\n \/<\/span>\n MRP4 Vesicle Kit<\/strong>\n <\/div>\n\n
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\n SeamlessBio \u2014 Cell4Pharma DMPK Assays<\/span>\n

Human MRP4 Vesicle Kit<\/h1>\n

\n HEK293-derived inside-out membrane vesicles for MRP4 inhibition screening and drug-drug interaction (DDI) risk assessment, in line with FDA and EMA testing recommendations.\n <\/p>\n

\n Informationen anfordern<\/a>\n Im Shop ansehen<\/a>\n <\/div>\n <\/div>\n
\n \"Human\n
Platzhalter f\u00fcr Produktbild<\/div>\n <\/div>\n <\/div>\n\n
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\n 01<\/span>\n

Nucleotide Analogue Relevance<\/h3>\n

\n Particularly relevant for nucleotide-analogue antivirals and chemotherapeutics as both substrate and inhibitor.\n <\/p>\n <\/div>\n\n

\n 02<\/span>\n

Inside-Out Vesicles<\/h3>\n

\n HEK293-derived membrane vesicles with confirmed MRP4 expression and inside-out orientation for reliable, reproducible transport data.\n <\/p>\n <\/div>\n\n

\n 03<\/span>\n

100 Reactions per Kit<\/h3>\n

\n Each kit is sized for 100 reactions based on a 96-well plate\n format, suitable for IC50 determination and single-concentration\n screening.\n <\/p>\n <\/div>\n\n

\n 04<\/span>\n

Dry Ice Shipment<\/h3>\n

\n Shipped frozen at \u221280 \u00b0C on dry ice to preserve vesicle\n integrity and transport activity upon arrival.\n <\/p>\n <\/div>\n\n <\/div>\n\n

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Why MRP4 testing matters in DMPK programs<\/h2>\n

\n MRP4 (ABCC4) is a broadly expressed transporter with substrate specificity for cyclic nucleotides, bile acids and a range of nucleotide-analogue drugs.\n <\/p>\n

\n The vesicle-based assay uses cyclic AMP or estrone sulfate as a probe substrate to quantify MRP4-mediated transport into inside-out membrane vesicles, in the presence and absence of test compounds, allowing determination of inhibition potency and IC50 values.\n <\/p>\n

\n MRP4 inhibition is relevant both to hepatic bile acid efflux (alongside MRP3 as a compensatory pathway) and to the disposition of nucleotide-analogue antivirals and chemotherapeutics that are direct MRP4 substrates. MRP4 is one of several transporters assessed when a candidate compound is itself a nucleotide analogue, since MRP4 may mediate its own efflux as well as inhibit endogenous bile acid transport.\n <\/p>\n <\/div>\n\n

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Typical applications<\/h3>\n