{"id":1626,"date":"2026-06-26T15:29:53","date_gmt":"2026-06-26T14:29:53","guid":{"rendered":"http:\/\/seamlessbio.de\/?page_id=1626"},"modified":"2026-07-06T21:33:34","modified_gmt":"2026-07-06T20:33:34","slug":"vaccine-production","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/vaccine-production\/","title":{"rendered":"Application Vaccine Production"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"1626\" class=\"elementor elementor-1626\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c5df693 e-con e-atomic-element e-flexbox-base e-6dd67ce \" data-id=\"c5df693\" data-element_type=\"e-flexbox\" data-e-type=\"e-flexbox\" data-interaction-id=\"c5df693\">\n    \t\t<div class=\"elementor-element elementor-element-196cf06 elementor-widget elementor-widget-html\" data-id=\"196cf06\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t\t<!-- YOAST SEO SETTINGS\nSlug:             applications\/vaccine-production\nFocus Keyword:    vaccine production Vero MDCK BHK-21 NCS calf serum serum selection\nSEO Title (EN):   Vaccine Production \u2014 Serum & Single-Use Bags for Vero, MDCK, BHK-21 | SeamlessBio\nMeta Desc (EN):   Serum selection guide for viral vaccine production. NCS vs Calf Serum vs FBS. Vero, MDCK, BHK-21. Australian\/US origin BSE\/TSE documentation. Rocker bags for scale-up. SeamlessBio.\n-->\n<section class=\"sb-app\">\n<style>\n.sb-app,.sb-app *,.sb-app *::before,.sb-app *::after{box-sizing:border-box;}\n.sb-app{background:#fff;padding:70px 40px 90px;font-family:Arial,sans-serif;color:#202020;}\n.sb-app .wrap{max-width:1200px;width:100%;margin:0 auto;}\n.sb-app .bc{display:flex;flex-wrap:wrap;gap:8px;margin:0 0 24px;color:#777;font-size:14px;}\n.sb-app .bc a{color:#18b7b2!important;text-decoration:none!important;font-weight:700;}\n.sb-app .bc strong{color:#111;font-weight:800;}\n.sb-app .hero{padding:64px 56px;margin-bottom:48px;border-radius:30px;background:linear-gradient(135deg,#18b7b2 0%,#65d3cf 52%,#e9fbfa 100%);color:#fff;}\n.sb-app .hero .ey{display:inline-block;margin-bottom:14px;font-size:13px;font-weight:900;text-transform:uppercase;letter-spacing:1.8px;}\n.sb-app .hero h1{margin:0 0 18px;color:#fff;font-size:clamp(26px,3.2vw,40px);line-height:1.1;font-weight:900;letter-spacing:-1px;max-width:840px;}\n.sb-app .hero .sub{max-width:720px;margin:0 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.ctab2{display:inline-flex;align-items:center;justify-content:center;min-height:46px;padding:0 28px;border-radius:999px;font-size:13px;font-weight:900;text-transform:uppercase;letter-spacing:.6px;text-decoration:none!important;transition:all .25s;}\n.sb-app .ctab1{background:#18b7b2;color:#fff!important;}\n.sb-app .ctab1:hover{background:#139b97;transform:translateY(-2px);}\n.sb-app .ctab2{background:transparent;border:2px solid #18b7b2;color:#18b7b2!important;}\n.sb-app .ctab2:hover{background:#18b7b2;color:#fff!important;transform:translateY(-2px);}\n@media(max-width:1100px){.sb-app .hero{padding:48px 36px;}.sb-app .rel-grid{grid-template-columns:1fr;}}\n@media(max-width:700px){.sb-app{padding:50px 16px 70px;}.sb-app .hero{padding:40px 24px;border-radius:24px;}.sb-app .hero h1{font-size:25px;}.sb-app .hbtns,.sb-app .ctabtns{flex-direction:column;}.sb-app .btn1,.sb-app .btn2,.sb-app .ctab1,.sb-app .ctab2{width:100%;}.sb-app .sec,.sb-app .faq{padding:20px;}.sb-app .cta{padding:36px 20px;}}\n<\/style>\n<div class=\"wrap\">\n  <div class=\"bc\">\n    <a href=\"http:\/\/seamlessbio.de\/\">Home<\/a><span>\/<\/span>\n    <a href=\"https:\/\/www.seamlessbio.de\/applications\/\">Applications<\/a><span>\/<\/span>\n    <strong>Vaccine Production<\/strong>\n  <\/div>\n  <div class=\"hero\">\n    <span class=\"ey\">SeamlessBio \u2014 Vero \u00b7 MDCK \u00b7 BHK-21 \u00b7 NCS \u00b7 Calf Serum \u00b7 FBS<\/span>\n    <h1>Vaccine Production \u2014 Serum &amp; Single-Use Bag Selection for Vero, MDCK &amp; BHK-21<\/h1>\n    <p class=\"sub\">Viral vaccine manufacturing relies on continuous cell lines as the primary production platform \u2014 Vero for rabies, polio and dengue; MDCK for influenza; BHK-21 for foot-and-mouth disease and veterinary vaccines. Each cell line has specific serum requirements that directly impact virus propagation yield and regulatory compliance. Post-COVID, serum supply chain reliability has become a critical raw material risk.<\/p>\n    <div class=\"hbtns\">\n      <a href=\"https:\/\/www.seamlessbio.de\/about-us\/contact-imprint\/\" class=\"btn1\">Contact Us<\/a>\n      <a href=\"https:\/\/shop.seamlessbio.de\" class=\"btn2\">View in Shop<\/a>\n    <\/div>\n  <\/div>\n  <div class=\"note\">\n    <strong>Cost optimisation in vaccine production:<\/strong> Newborn Calf Serum (NCS) and Calf Serum (CS) are the preferred cost-effective alternatives to FBS for Vero, MDCK and BHK-21 cell maintenance and virus propagation \u2014 equivalent growth support at 30\u201360% lower cost.\n  <\/div>\n\n  <!-- CELL LINE TABLE -->\n  <div class=\"sec\">\n    <h2>Cell Line \u2014 Serum Selection Guide<\/h2>\n    <div class=\"tw\">\n      <table>\n        <thead><tr><th>Cell Line<\/th><th>Vaccine Application<\/th><th>Recommended Serum<\/th><th>Concentration<\/th><\/tr><\/thead>\n        <tbody>\n          <tr>\n            <td><strong>Vero<\/strong><\/td>\n            <td>Rabies (inactivated), Polio (IPV\/OPV), Dengue, Japanese Encephalitis, Yellow Fever, SARS-CoV-2<\/td>\n            <td><a href=\"https:\/\/www.seamlessbio.de\/products\/animal-sera\/bovine-sera-and-more\/newborn-calf-serum-ncs\">NCS \u226410 days \u2014 AU or US origin<\/a><\/td>\n            <td>5\u201310% for maintenance; 2\u20135% during virus propagation<\/td>\n          <\/tr>\n          <tr>\n            <td><strong>MDCK<\/strong><\/td>\n            <td>Influenza (seasonal and pandemic), respiratory virus research<\/td>\n            <td><a href=\"https:\/\/www.seamlessbio.de\/products\/animal-sera\/bovine-sera-and-more\/newborn-calf-serum-ncs\">NCS \u226410 days \u2014 AU or US origin<\/a><\/td>\n            <td>5\u201310% for cell maintenance; serum-free for virus infection step (trypsin-dependent influenza)<\/td>\n          <\/tr>\n          <tr>\n            <td><strong>BHK-21<\/strong><\/td>\n            <td>Foot-and-mouth disease (FMD), rabies, veterinary virus vaccines<\/td>\n            <td><a href=\"https:\/\/www.seamlessbio.de\/products\/animal-sera\/bovine-sera-and-more\/calf-serum-cs\">CS \u2014 AU or US origin<\/a><\/td>\n            <td>5\u201310% for routine culture; 2\u20135% during production phase<\/td>\n          <\/tr>\n          <tr>\n            <td><strong>HEK293<\/strong><\/td>\n            <td>Adenoviral vectors for vaccine delivery, recombinant subunit antigens<\/td>\n            <td><a href=\"https:\/\/www.seamlessbio.de\/products\/fetal-bovine-serum\/fbs-low-very-low-endotoxin\">FBS Low Endotoxin \u22645 EU\/mL<\/a><\/td>\n            <td>10% for maintenance; reduce to 2% or serum-free at production<\/td>\n          <\/tr>\n          <tr>\n            <td><strong>MRC-5 \/ WI-38 (diploid)<\/strong><\/td>\n            <td>Varicella, hepatitis A, rabies (human diploid cell vaccine)<\/td>\n            <td><a href=\"https:\/\/www.seamlessbio.de\/products\/fetal-bovine-serum\/fbs-low-very-low-endotoxin\">FBS Low Endotoxin \u22645 EU\/mL<\/a><\/td>\n            <td>10% \u2014 diploid cells require FBS quality; NCS\/CS not recommended for primary diploid strains<\/td>\n          <\/tr>\n        <\/tbody>\n      <\/table>\n    <\/div>\n  <\/div>\n\n  <!-- NCS vs CS vs FBS -->\n  <div class=\"sec\">\n    <h2>NCS vs Calf Serum vs FBS \u2014 Cost Optimisation<\/h2>\n    <div class=\"tw\">\n      <table>\n        <thead><tr><th>Serum<\/th><th>Donor Age<\/th><th>Cost vs FBS<\/th><th>Best For<\/th><th>Not Suitable For<\/th><\/tr><\/thead>\n        <tbody>\n          <tr>\n            <td><a href=\"https:\/\/www.seamlessbio.de\/products\/animal-sera\/bovine-sera-and-more\/newborn-calf-serum-ncs\"><strong>NCS \u226410 days<\/strong><\/a><\/td>\n            <td>\u226410 days<\/td>\n            <td>~30\u201350% lower<\/td>\n            <td>Vero, MDCK, BHK-21 routine maintenance and virus propagation<\/td>\n            <td>Diploid cell strains (MRC-5, WI-38), sensitive primary cultures<\/td>\n          <\/tr>\n          <tr>\n            <td><a href=\"https:\/\/www.seamlessbio.de\/products\/animal-sera\/bovine-sera-and-more\/calf-serum-cs\"><strong>Calf Serum (CS)<\/strong><\/a><\/td>\n            <td>14 days \u2013 6 months<\/td>\n            <td>~40\u201360% lower<\/td>\n            <td>BHK-21 large-scale FMD\/veterinary vaccine production. High-volume cell banking.<\/td>\n            <td>Diploid cells, applications requiring low IgG<\/td>\n          <\/tr>\n          <tr>\n            <td><a href=\"https:\/\/www.seamlessbio.de\/products\/fetal-bovine-serum\/fbs-low-very-low-endotoxin\"><strong>FBS Low Endotoxin<\/strong><\/a><\/td>\n            <td>Fetal<\/td>\n            <td>Reference<\/td>\n            <td>Diploid cell strains, HEK293 adenoviral vectors, CHO recombinant antigens, sensitive primary cultures<\/td>\n            <td>Cost-sensitive bulk Vero\/MDCK\/BHK-21 production where NCS\/CS perform equivalently<\/td>\n          <\/tr>\n        <\/tbody>\n      <\/table>\n    <\/div>\n  <\/div>\n\n  <!-- ORIGIN -->\n  <div class=\"sec\">\n    <h2>Origin Selection \u2014 BSE\/TSE Documentation for Vaccine Submissions<\/h2>\n    <div class=\"tw\">\n      <table>\n        <thead><tr><th>Origin<\/th><th>BSE\/TSE Status<\/th><th>Documentation<\/th><th>Best For<\/th><\/tr><\/thead>\n        <tbody>\n          <tr><td><strong>Australian<\/strong><\/td><td>OIE Negligible Risk \u2014 no indigenous BSE<\/td><td>EDQM CEP available, USDA import permitted<\/td><td>EU and US regulatory submissions requiring maximum TSE\/BSE documentation. WHO-prequalified vaccine programmes.<\/td><\/tr>\n          <tr><td><strong>US Origin<\/strong><\/td><td>USDA 9CFR licensed<\/td><td>9CFR CoA, FDA-aligned<\/td><td>US regulatory submissions. Cost-effective option for USDA-documented programmes.<\/td><\/tr>\n          <tr><td><strong>EU Origin<\/strong><\/td><td>EU TSE\/BSE surveillance \u2014 controlled risk<\/td><td>EDQM CEP available<\/td><td>EU regulatory submissions. DACH market documentation requirements.<\/td><\/tr>\n        <\/tbody>\n      <\/table>\n    <\/div>\n  <\/div>\n\n  <!-- PRODUCTION PHASE MAP -->\n  <div class=\"sec\">\n    <h2>Vaccine Production Phases \u2014 Complete Product Map<\/h2>\n    <div class=\"tw\">\n      <table>\n        <thead><tr><th>Phase<\/th><th>Step<\/th><th>Recommended Product<\/th><\/tr><\/thead>\n        <tbody>\n          <tr><td><span class=\"pl\">Cell Bank<\/span><\/td><td>MCB and WCB preparation for Vero, MDCK, BHK-21<\/td><td><a href=\"https:\/\/www.seamlessbio.de\/products\/animal-sera\/bovine-sera-and-more\/newborn-calf-serum-ncs\">NCS AU origin<\/a> \u2014 highest BSE documentation for cell bank regulatory filing<\/td><\/tr>\n          <tr><td><span class=\"pl\">Seed Train<\/span><\/td><td>Cell expansion from WCB to production density<\/td><td><a href=\"https:\/\/www.seamlessbio.de\/products\/animal-sera\/bovine-sera-and-more\/newborn-calf-serum-ncs\">NCS or CS<\/a> at 5\u201310%. <a href=\"https:\/\/www.seamlessbio.de\/products\/single-use-bioprocessing\/rocker-bags\">Rocker Bags<\/a> for suspension seed stages.<\/td><\/tr>\n          <tr><td><span class=\"pl\">Infection<\/span><\/td><td>Virus inoculation and propagation<\/td><td>Reduce to 2\u20135% or switch to serum-free at infection step \u2014 reduces serum interference with virus quantification assays<\/td><\/tr>\n          <tr><td><span class=\"pl\">Harvest<\/span><\/td><td>Virus harvest, clarification, primary purification<\/td><td><a href=\"https:\/\/www.seamlessbio.de\/products\/single-use-bioprocessing\/2d-bioprocessing-bags\">2D Single-Use Bags<\/a> for closed virus pool collection and storage<\/td><\/tr>\n          <tr><td><span class=\"pl\">Formulation<\/span><\/td><td>Virus concentration, formulation, filling<\/td><td><a href=\"https:\/\/www.seamlessbio.de\/products\/albumin-proteins\/bsa-low-endotoxin\">BSA Low Endotoxin<\/a> in stabilisation buffer if required<\/td><\/tr>\n        <\/tbody>\n      <\/table>\n    <\/div>\n  <\/div>\n\n  <!-- SINGLE-USE BAGS -->\n  <div class=\"sec\">\n    <h2>Single-Use Bags for Vaccine Manufacturing<\/h2>\n    <div class=\"tw\">\n      <table>\n        <thead><tr><th>Product<\/th><th>Volume<\/th><th>Vaccine Application<\/th><th>Key Specification<\/th><\/tr><\/thead>\n        <tbody>\n          <tr><td><a href=\"https:\/\/www.seamlessbio.de\/products\/single-use-bioprocessing\/rocker-bags\">Rocker Bag<\/a><\/td><td>0.5\u2013200 L<\/td><td>Vero or MDCK cell expansion in rocking wave bioreactor for virus seed preparation. BHK-21 FMD scale-up.<\/td><td>USP Class VI. Gamma sterilised \u226525 kGy ISO 11137. Corning and HyPerforma compatible. ISO 13485.<\/td><\/tr>\n          <tr><td><a href=\"https:\/\/www.seamlessbio.de\/products\/single-use-bioprocessing\/2d-bioprocessing-bags\">2D Single-Use Bags<\/a><\/td><td>150 mL\u201350 L<\/td><td>Virus harvest collection, media preparation, buffer storage, intermediate virus pools<\/td><td>USP Class VI. Gamma sterilised. Drop-in Sartorius Flexboy\u00ae. GMP documentation. ISO 13485.<\/td><\/tr>\n          <tr><td><a href=\"https:\/\/www.seamlessbio.de\/products\/single-use-bioprocessing\/3d-bioprocessing-bags\">3D Single-Use Bags<\/a><\/td><td>50\u20131,000 L<\/td><td>Large-volume media preparation for clinical-scale vaccine manufacturing<\/td><td>MPX tubing. GMP documentation package. Gamma sterilised. ISO 13485.<\/td><\/tr>\n        <\/tbody>\n      <\/table>\n    <\/div>\n  <\/div>\n\n  <!-- FAQ -->\n  <div class=\"faq\">\n    <h2>Frequently Asked Questions<\/h2>\n    <div class=\"faq-item\">\n      <div class=\"faq-q\">Can NCS replace FBS for Vero cell vaccine production?<\/div>\n      <div class=\"faq-a\">Yes \u2014 for routine Vero cell maintenance and virus propagation (rabies, polio, dengue), NCS performs equivalently to FBS at 30\u201350% lower cost. NCS from calves \u226410 days provides a rich growth factor composition that supports Vero cell health and virus yield. Australian origin NCS provides the strongest regulatory documentation for WHO-prequalified vaccine programmes.<\/div>\n    <\/div>\n    <div class=\"faq-item\">\n      <div class=\"faq-q\">Why is serum reduced or removed at the virus infection step?<\/div>\n      <div class=\"faq-a\">High serum concentrations at the virus infection step can interfere with virus adsorption to cell surface receptors \u2014 reducing infection efficiency. Additionally, serum proteins interfere with plaque assay and TCID50 quantification of virus yield. Reducing serum to 2% or switching to serum-free medium during virus propagation is standard practice for Vero and MDCK-based vaccine production.<\/div>\n    <\/div>\n    <div class=\"faq-item\">\n      <div class=\"faq-q\">Which origin NCS for WHO-prequalified vaccine submissions?<\/div>\n      <div class=\"faq-a\">Australian origin NCS is the preferred choice \u2014 OIE negligible BSE\/TSE risk, EDQM CEP available, USDA import permitted. Australian origin provides the strongest regulatory documentation for both EU (EMA) and US (FDA) submissions and WHO prequalification dossiers.<\/div>\n    <\/div>\n    <div class=\"faq-item\">\n      <div class=\"faq-q\">What single-use bag for virus harvest collection?<\/div>\n      <div class=\"faq-a\">2D Single-Use Bags in 2\u201310 L format are standard for virus harvest collection from Vero or MDCK roller bottle or bioreactor runs. Closed system collection directly into gamma-sterilised bags minimises contamination risk and reduces handling steps. 3D bags in 50\u2013200 L are used for pooled harvest intermediates in large-scale manufacturing.<\/div>\n    <\/div>\n  <\/div>\n\n  <div class=\"related\">\n    <h3>Related Applications &amp; Products<\/h3>\n    <div class=\"rel-grid\">\n      <a href=\"https:\/\/www.seamlessbio.de\/applications\/aav-viral-vector-production\">\u2192 AAV &amp; Viral Vector Production<\/a>\n      <a href=\"https:\/\/www.seamlessbio.de\/products\/single-use-bioprocessing\">\u2192 Single-Use Bioprocessing Bags<\/a>\n      <a href=\"https:\/\/www.seamlessbio.de\/products\/animal-sera\/bovine-sera-and-more\/newborn-calf-serum-ncs\">\u2192 Newborn Calf Serum (NCS)<\/a>\n      <a href=\"https:\/\/www.seamlessbio.de\/products\/animal-sera\/bovine-sera-and-more\/calf-serum-cs\">\u2192 Calf Serum (CS)<\/a>\n      <a href=\"https:\/\/www.seamlessbio.de\/products\/fetal-bovine-serum\/fbs-low-very-low-endotoxin\">\u2192 FBS Low Endotoxin \u22645 EU\/mL<\/a>\n      <a href=\"https:\/\/www.seamlessbio.de\/products\/albumin-proteins\/bsa-low-endotoxin\">\u2192 BSA Low Endotoxin<\/a>\n    <\/div>\n  <\/div>\n  <div class=\"hub-back\"><a href=\"https:\/\/www.seamlessbio.de\/applications\/\">\u2190 All Application Guides Overview<\/a><\/div>\n  <div class=\"cta\">\n    <h2>Request Free Test Volumes &amp; Origin Documentation<\/h2>\n    <p>NCS and CS samples with Australian\/US BSE documentation. Lot-specific CoA and CoO. Batch reservation for vaccine manufacturing campaigns.<br>Email: info@seamlessbio.de | +49 851 37932226<\/p>\n    <div class=\"ctabtns\">\n      <a href=\"mailto:info@seamlessbio.de\" class=\"ctab1\">Contact Us<\/a>\n      <a href=\"https:\/\/shop.seamlessbio.de\" class=\"ctab2\">View in Shop<\/a>\n    <\/div>\n  <\/div>\n<\/div>\n<script type=\"application\/ld+json\">\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@graph\": [\n    {\n      \"@type\": \"FAQPage\",\n      \"@id\": \"https:\/\/www.seamlessbio.de\/applications\/vaccine-production#faqpage\",\n      \"mainEntity\": [\n    {\n      \"@type\": \"Question\",\n      \"name\": \"Can NCS replace FBS for Vero cell vaccine production?\",\n      \"acceptedAnswer\": {\n        \"@type\": \"Answer\",\n        \"text\": \"Yes \u2014 for routine Vero cell maintenance and virus propagation (rabies, polio, dengue), NCS performs equivalently to FBS at 30\u201350% lower cost. 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Closed system collection directly into gamma-sterilised bags minimises contamination risk and reduces handling steps. 3D bags in 50\u2013200 L are used for pooled harvest intermediates in large-scale manufacturing.\"\n      }\n    }\n      ]\n    },\n    {\n      \"@type\": \"BreadcrumbList\",\n      \"@id\": \"https:\/\/www.seamlessbio.de\/applications\/vaccine-production#breadcrumb\",\n      \"itemListElement\": [\n        {\n          \"@type\": \"ListItem\",\n          \"position\": 1,\n          \"name\": \"Home\",\n          \"item\": \"https:\/\/www.seamlessbio.de\/\"\n        },\n        {\n          \"@type\": \"ListItem\",\n          \"position\": 2,\n          \"name\": \"Applications\",\n          \"item\": \"https:\/\/www.seamlessbio.de\/applications\/\"\n        },\n        {\n          \"@type\": \"ListItem\",\n          \"position\": 3,\n          \"name\": \"Vaccine Production\",\n          \"item\": \"https:\/\/www.seamlessbio.de\/applications\/vaccine-production\"\n        }\n      ]\n    }\n  ]\n}\n<\/script>\n<\/section>\t\t\t\t<\/div>\n\t\t\n<\/div>\n\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Home\/ Applications\/ Vaccine Production SeamlessBio \u2014 Vero \u00b7 MDCK \u00b7 BHK-21 \u00b7 NCS \u00b7 Calf Serum \u00b7 FBS Vaccine Production \u2014 Serum &amp; Single-Use Bag Selection for Vero, MDCK &amp; [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","footnotes":""},"class_list":["post-1626","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Biologicals for Vaccine Production | SeamlessBio<\/title>\n<meta name=\"description\" content=\"Virus-tested &amp; gamma-irradiated FBS plus production-scale single-use systems for vaccine manufacturing. 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