{"id":1931,"date":"2026-06-27T17:58:33","date_gmt":"2026-06-27T16:58:33","guid":{"rendered":"http:\/\/seamlessbio.de\/?page_id=1931"},"modified":"2026-07-02T22:21:41","modified_gmt":"2026-07-02T21:21:41","slug":"seperation-solution","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/seperation-solution\/","title":{"rendered":"Trennl\u00f6sung"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"1931\" class=\"elementor elementor-1931\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-7fa98bd e-con e-atomic-element e-flexbox-base e-711737f \" data-id=\"7fa98bd\" data-element_type=\"e-flexbox\" data-e-type=\"e-flexbox\" data-interaction-id=\"7fa98bd\">\n    \t\t<div class=\"elementor-element elementor-element-9ecab74 elementor-widget elementor-widget-html\" data-id=\"9ecab74\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t\t<!--\nYOAST SEO\nSlug:      \/products\/cell-culture-media-reagents\/separation-solution\/\nSEO Title: Separation Solution | Density Gradient PBMC Isolation | SeamlessBio\nMeta Desc: Density gradient separation solution for PBMC isolation, cell separation and buoyant density applications. Endotoxin-tested, sterile filtered. All on request. SeamlessBio.\n-->\n<script type=\"application\/ld+json\">\n{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"BreadcrumbList\",\"itemListElement\":[{\"@type\": \"ListItem\", \"position\": 1, \"name\": \"Home\", \"item\": \"https:\/\/www.seamlessbio.de\/\"}, {\"@type\": \"ListItem\", \"position\": 2, \"name\": \"Cell Culture Media\", \"item\": \"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/\"}]},{\"@type\":\"FAQPage\",\"mainEntity\":[{\"@type\": \"Question\", \"name\": \"What is separation solution used for in cell culture?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Density gradient separation solution is used to isolate peripheral blood mononuclear cells (PBMCs) from whole blood or buffy coat by density gradient centrifugation. The cells layer at the interface between plasma and the separation medium based on their buoyant density. It is also used for general cell separation by density in research workflows.\"}}, {\"@type\": \"Question\", \"name\": \"What is the difference between separation solution and Ficoll?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Ficoll-based density gradient media are the standard for PBMC isolation \\u2014 SeamlessBio separation solution is a polysucrose\/sodium diatrizoate-based formulation with equivalent performance. It provides a defined density (1.077 g\/mL) for mononuclear cell isolation from human or animal blood.\"}}, {\"@type\": \"Question\", \"name\": \"Is SeamlessBio separation solution endotoxin-tested?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Yes \\u2014 separation solution is sterile filtered (0.2 \\u00b5m) and endotoxin-tested by the LAL method. Certificate of Analysis provided per lot.\"}}]}]}\n<\/script>\n<div class=\"sbp-page\">\n<div class=\"sbp-wrap\">\n<nav class=\"sbp-bc\"><a href=\"https:\/\/www.seamlessbio.de\/\">Home<\/a><span>\/<\/span><a href=\"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/\">Cell Culture Media<\/a><span>\/<\/span><strong>Separation Solution<\/strong><\/nav>\n<div class=\"sbp-hero\">\n  <span class=\"sbp-eyebrow\">Cell Culture Reagents \u00b7 Density Gradient \u00b7 PBMC Isolation<\/span>\n  <h1>Separation Solution<\/h1>\n  <p>Density gradient separation solution for PBMC isolation from whole blood or buffy coat, cell separation by buoyant density and related research workflows. Endotoxin-tested, sterile filtered, Certificate of Analysis per lot.<\/p>\n  <div class=\"sbp-tags\"><span>Density 1.077 g\/mL<\/span><span>Sterile Filtered<\/span><span>Endotoxin Tested<\/span><span>PBMC Isolation<\/span><span>All on Request<\/span><\/div>\n  <div class=\"sbp-btns\"><a href=\"mailto:info@seamlessbio.de?subject=Separation%20Solution%20Enquiry\" class=\"sbp-btn\">Request Quote<\/a><a href=\"https:\/\/www.seamlessbio.de\/applications\/pbmc-immunology\/\" class=\"sbp-btn-o\">PBMC Application Guide<\/a><\/div>\n<\/div>\n<div class=\"sbp-section\">\n<h2 class=\"sbp-h2\">Product Details<\/h2>\n<table class=\"sbp-table\">\n<tr><th>Parameter<\/th><th>Value<\/th><\/tr>\n<tr><td>Density<\/td><td>1.077 g\/mL \u00b1 0.001 at 20\u00b0C<\/td><\/tr>\n<tr><td>Osmolality<\/td><td>~280 mOsm\/kg<\/td><\/tr>\n<tr><td>pH<\/td><td>7.2 \u2013 7.4<\/td><\/tr>\n<tr><td>Sterility<\/td><td>Sterile filtered (0.2 \u00b5m)<\/td><\/tr>\n<tr><td>Endotoxin<\/td><td>\u2264 1 EU\/mL (LAL method)<\/td><\/tr>\n<tr><td>Appearance<\/td><td>Clear, colourless to pale yellow solution<\/td><\/tr>\n<tr><td>Packaging<\/td><td>100 mL, 500 mL \u2014 other volumes on request<\/td><\/tr>\n<tr><td>Storage<\/td><td>+2\u00b0C to +8\u00b0C, protect from light<\/td><\/tr>\n<tr><td>Shelf life<\/td><td>24 months from date of manufacture<\/td><\/tr>\n<tr><td>Availability<\/td><td>On request<\/td><\/tr>\n<\/table>\n<\/div>\n<div class=\"sbp-alt\">\n<h2 class=\"sbp-h2\">Applications<\/h2>\n<div class=\"sbp-grid-3\">\n<div class=\"sbp-card\"><h3>PBMC Isolation<\/h3><p>Isolation of peripheral blood mononuclear cells from human or animal whole blood and buffy coat by density gradient centrifugation at 400\u2013800 \u00d7 g.<\/p><\/div>\n<div class=\"sbp-card\"><h3>T-Cell & NK-Cell Research<\/h3><p>Preparation of lymphocyte-enriched fractions for T-cell activation assays, ELISPOT, mixed lymphocyte reactions and cytotoxicity assays.<\/p><\/div>\n<div class=\"sbp-card\"><h3>Monocyte Isolation<\/h3><p>Density-based enrichment of monocytes from PBMC fractions for macrophage differentiation, phagocytosis assays and innate immune research.<\/p><\/div>\n<div class=\"sbp-card\"><h3>Bone Marrow Processing<\/h3><p>Mononuclear cell isolation from bone marrow aspirates for haematopoietic stem cell research and MSC isolation protocols.<\/p><\/div>\n<div class=\"sbp-card\"><h3>CAR-T Manufacturing<\/h3><p>PBMC isolation as the first step in T-cell activation and transduction workflows for CAR-T cell manufacturing processes.<\/p><\/div>\n<div class=\"sbp-card\"><h3>General Cell Density Separation<\/h3><p>Separation of cell populations by buoyant density in any application requiring removal of dead cells, red blood cells or debris.<\/p><\/div>\n<\/div>\n<\/div>\n<div class=\"sbp-section\">\n<h2 class=\"sbp-h2\">Frequently Asked Questions<\/h2>\n<div class=\"sbp-faq\"><h3>What is separation solution used for?<\/h3><p>Density gradient separation solution isolates PBMCs from whole blood or buffy coat by density gradient centrifugation. Cells layer at the interface based on buoyant density \u2014 mononuclear cells (1.070\u20131.077 g\/mL) remain at the interface while erythrocytes and granulocytes pellet.<\/p><\/div>\n<div class=\"sbp-faq\"><h3>Is it endotoxin-tested?<\/h3><p>Yes \u2014 sterile filtered (0.2 \u00b5m) and endotoxin-tested by LAL method (\u22641 EU\/mL). CoA provided per lot.<\/p><\/div>\n<div class=\"sbp-faq\"><h3>What volumes are available?<\/h3><p>100 mL and 500 mL as standard. Other volumes on request \u2014 contact us with your requirement.<\/p><\/div>\n<\/div>\n<div class=\"sbp-section\">\n<h2 class=\"sbp-h2\">Related Products<\/h2>\n<div class=\"sbp-grid-4\">\n<a href=\"https:\/\/www.seamlessbio.de\/products\/human-sera-plasma-and-more\/\" class=\"sbp-card-link\"><h3>Human Serum & Plasma<\/h3><p>Human serum variants for immunology and cell culture applications.<\/p><\/a>\n<a href=\"https:\/\/www.seamlessbio.de\/applications\/pbmc-immunology\/\" class=\"sbp-card-link\"><h3>PBMC & Immunology<\/h3><p>Application guide for PBMC isolation and immunological assays.<\/p><\/a>\n<a href=\"https:\/\/www.seamlessbio.de\/applications\/car-t-cell-manufacturing\/\" class=\"sbp-card-link\"><h3>CAR-T Manufacturing<\/h3><p>Human serum and reagents for T-cell expansion and CAR-T production.<\/p><\/a>\n<a href=\"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/\" class=\"sbp-card-link\"><h3>Cell Culture Media<\/h3><p>Complete cell culture reagent range.<\/p><\/a>\n<\/div>\n<\/div>\n<div class=\"sbp-cta\"><h2>Request Separation Solution<\/h2><p>On request \u2014 contact us with your required volume and application.<\/p><div class=\"sbp-cta-btns\"><a href=\"https:\/\/www.seamlessbio.de\/about-us\/contact-imprint\/\" class=\"sbp-btn\">Contact Us<\/a><a href=\"mailto:info@seamlessbio.de?subject=Separation%20Solution\" class=\"sbp-btn-o\">info@seamlessbio.de<\/a><\/div><\/div>\n<\/div>\n<\/div>\n<style>\n.sbp-page,.sbp-page *{box-sizing:border-box}\n.sbp-page{font-family:Arial,sans-serif;color:#202020;background:#fff;padding:60px 0 90px}\n.sbp-wrap{max-width:1400px;margin:0 auto;padding:0 40px}\n.sbp-bc{display:flex;flex-wrap:wrap;gap:6px;align-items:center;font-size:13px;color:#999;margin-bottom:32px}\n.sbp-bc a{color:#18b7b2!important;text-decoration:none!important;font-weight:600}\n.sbp-bc span{color:#ddd}\n.sbp-hero{background:#f7fbfb;border:1px solid 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[&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","footnotes":""},"class_list":["post-1931","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Cell Dissociation &amp; Separation Solutions | Trypsin, EDTA, Accutase<\/title>\n<meta name=\"description\" content=\"Cell dissociation and separation solutions for cell culture \u2014 trypsin-EDTA, accutase, collagenase and custom enzymatic solutions.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/seamlessbio.de\/de\/seperation-solution\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta 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