{"id":2878,"date":"2026-08-22T15:44:28","date_gmt":"2026-08-22T14:44:28","guid":{"rendered":"https:\/\/seamlessbio.de\/?page_id=2878"},"modified":"2026-08-22T15:45:17","modified_gmt":"2026-08-22T14:45:17","slug":"glossary","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/glossary\/","title":{"rendered":"Glossary"},"content":{"rendered":"
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Quick definitions for the most commonly misunderstood terms in cell culture, DMPK transporter assays and bioprocessing \u2014 written for researchers, DMPK scientists and procurement teams.<\/p>\n\n

Cell Culture & Serum<\/h2>\n\n\n \n \n \n \n \n \n
Term<\/th>\n One-line definition<\/th>\n Full guide<\/th>\n <\/tr>\n
FBS (Fetal Bovine Serum)<\/a><\/strong><\/td>\n The most widely used cell culture supplement \u2014 blood serum collected from bovine fetuses, providing growth factors, albumin and nutrients to support cell growth.<\/td>\n Read \u2192<\/a><\/td>\n <\/tr>\n
Heat Inactivated FBS<\/a><\/strong><\/td>\n FBS heated to 56\u00b0C for 30 minutes to destroy complement proteins \u2014 used in embryo culture, PBMC assays and virus neutralisation.<\/td>\n Read \u2192<\/a><\/td>\n <\/tr>\n
Geringer Endotoxingehalt FBS<\/a><\/strong><\/td>\n FBS tested to <1 EU\/mL endotoxin \u2014 required for drug screening, AAV production and cytokine assays where LPS would confound results.<\/td>\n Read \u2192<\/a><\/td>\n <\/tr>\n
Lysat aus menschlichen Blutpl\u00e4ttchen (hPL)<\/a><\/strong><\/td>\n A xeno-free, human-derived FBS alternative produced by freeze-thaw lysis of platelets \u2014 releasing concentrated PDGF, EGF, TGF-\u03b2 and IGF-1 for MSC and primary cell culture.<\/td>\n Read \u2192<\/a><\/td>\n <\/tr>\n
Off-the-Clot (OTC) Serum<\/a><\/strong><\/td>\n Human serum produced by natural clotting \u2014 platelets fully activate and release growth factors, giving higher PDGF and EGF than standard plasma-derived serum.<\/td>\n Read \u2192<\/a><\/td>\n <\/tr>\n<\/table>\n\n

Serum-Free & Albumin<\/h2>\n\n\n \n \n \n \n
Term<\/th>\n One-line definition<\/th>\n Full guide<\/th>\n <\/tr>\n
Xeno-Free Cell Culture<\/a><\/strong><\/td>\n Cell culture containing no components from a species other than the one being cultured \u2014 for human cells, no bovine or murine materials; human-derived supplements (hPL, rHSA) are acceptable.<\/td>\n Read \u2192<\/a><\/td>\n <\/tr>\n
rHSA (Recombinant Human Serum Albumin)<\/a><\/strong><\/td>\n Human serum albumin produced recombinantly in rice or yeast \u2014 animal-free, xeno-free, GMP-compatible carrier protein for serum-free cell culture and AAV production media.<\/td>\n Read \u2192<\/a><\/td>\n <\/tr>\n
Rekombinantes humanes Transferrin (OsrhTF)<\/a><\/strong><\/td>\n Animal-free iron delivery protein for serum-free medium \u2014 replaces the transferrin function of FBS; essential to prevent iron starvation during serum-free adaptation.<\/td>\n Read \u2192<\/a><\/td>\n <\/tr>\n<\/table>\n\n

DMPK & Drug Transporters<\/h2>\n\n\n \n \n \n \n \n
Term<\/th>\n One-line definition<\/th>\n Full guide<\/th>\n <\/tr>\n
BSEP (Bile Salt Export Pump)<\/a><\/strong><\/td>\n The primary hepatocyte canalicular transporter for bile salt secretion \u2014 BSEP inhibition causes intracellular bile salt accumulation and is the leading mechanism of cholestatic drug-induced liver injury (DILI).<\/td>\n Read \u2192<\/a><\/td>\n <\/tr>\n
P-glycoprotein (P-gp \/ MDR1 \/ ABCB1)<\/a><\/strong><\/td>\n An ABC efflux transporter expressed at the intestinal epithelium, blood-brain barrier and liver \u2014 limits oral bioavailability and CNS penetration; mandatory DDI testing for all NMEs under FDA, EMA and ICH M12.<\/td>\n Read \u2192<\/a><\/td>\n <\/tr>\n
Vesicular Transport Assay<\/a><\/strong><\/td>\n An in vitro method using inside-out membrane vesicles to measure ABC transporter substrate accumulation and inhibitor IC50 \u2014 the only viable method for BSEP, MRP2, MRP3, MRP4 where no cell monolayer model exists.<\/td>\n Read \u2192<\/a><\/td>\n <\/tr>\n
DILI (Drug-Induced Liver Injury)<\/a><\/strong><\/td>\n Hepatotoxicity caused by a drug or supplement \u2014 the leading cause of post-marketing drug withdrawal. BSEP inhibition is the primary mechanism of cholestatic DILI.<\/td>\n Read \u2192<\/a><\/td>\n <\/tr>\n<\/table>\n\n

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