{"id":2888,"date":"2026-08-22T15:46:43","date_gmt":"2026-08-22T14:46:43","guid":{"rendered":"https:\/\/wheat-quail-856400.hostingersite.com\/?page_id=2888"},"modified":"2026-11-12T18:44:50","modified_gmt":"2026-11-12T17:44:50","slug":"what-is-heat-inactivated-fbs","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/what-is-heat-inactivated-fbs\/","title":{"rendered":"Was ist hitzeinaktiviertes FBS?"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"2888\" class=\"elementor elementor-2888\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-6e4e661 e-con e-atomic-element e-flexbox-base e-default-div e-ff0a9d1\" data-id=\"6e4e661\" data-element_type=\"e-flexbox\" data-e-type=\"e-flexbox\" data-interaction-id=\"6e4e661\">\n    \t\t<div class=\"elementor-element elementor-element-fee1b0e elementor-widget elementor-widget-html\" data-id=\"fee1b0e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t\t<!-- \nWORDPRESS PAGE \u2014 PASTE INTO GUTENBERG \"BENUTZERDEFINIERTES HTML\" BLOCK\nYoast SEO:\n  Slug: what-is-heat-inactivated-fbs\n  SEO Title: What is Heat Inactivated FBS? \u2014 Definition & When to Use It | SeamlessBio\n  Meta Description: What is heat inactivated FBS, how is it made, what does heat inactivation remove, and when do you need it? Complete definition from SeamlessBio.\n  Focus Keyword: what is heat inactivated FBS\n  Meta Robots: index, follow\n  Page Type: Article\n-->\n\n<div style=\"background:#e9fbfa;border-left:4px solid #18b7b2;border-radius:0 8px 8px 0;padding:20px 24px;margin-bottom:32px;\">\n<p style=\"font-size:11px;font-weight:700;text-transform:uppercase;letter-spacing:1px;color:#139b97;margin-bottom:8px;\">Kurzdefinition<\/p>\n<p style=\"font-size:16px;margin:0;\"><strong>Was ist hitzeinaktiviertes FKS?<\/strong> Hitzeinaktiviertes FKS (HI-FKS) ist fetales K\u00e4lberserum, das 30 Minuten auf 56 \u00b0C erhitzt wurde, um die Komplementproteine zu zerst\u00f6ren. Es wird in Zellkulturanwendungen eingesetzt, in denen aktives Komplement Zellen lysieren oder Assayergebnisse st\u00f6ren w\u00fcrde.<\/p>\n<!-- BUY BOX (SEO: links to buy pages) -->\n<div class=\"sb-buy-box\" style=\"border:1px solid #cfe9e6;background:#f4fbfa;border-radius:12px;padding:22px 26px;margin:28px auto;max-width:1200px;box-sizing:border-box\">\n<h2 style=\"font-size:22px;line-height:1.3;margin:0 0 6px\">Fetales K\u00e4lberserum (FKS) ab EU-Lager kaufen<\/h2>\n<p style=\"margin:0 0 14px\">Angebot oder Testmuster anfordern \u2013 Lager in der EU, ohne Mindestmenge, Chargenreservierung bis zu 36 Monate.<\/p>\n<ul style=\"margin:0;padding-left:20px;columns:2 300px;column-gap:32px\">\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/fks-hitzeinaktiviert-usa-australien-suedamerika\/\" style=\"font-weight:700\">FKS hitzeinaktiviert<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/fetales-kaelberserum-fks-suedamerika\/\">Fetales K\u00e4lberserum \u2013 Herkunft S\u00fcdamerika<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/fetales-kaelberserum-fks-usa\/\">Fetales K\u00e4lberserum \u2013 Herkunft USA<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/fetales-kaelberserum-fks-australien\/\">Fetales K\u00e4lberserum \u2013 Herkunft Australien<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/fks-low-igg\/\">FKS Ultra Low IgG (&lt;5 \u00b5g\/ml)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/fks-tetrazyklinfrei-tet-free\/\">Tetrazyklinfreies FKS (Tet-free)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/fks-es-zell-getestet-suedamerika\/\">ES-Zell-getestetes FKS<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/fks-low-endotoxin-suedamerika\/\">FBS Geringer Endotoxingehalt (&lt;1 EU\/ml)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/fks-maennlich-testosteron-definiert\/\">FKS m\u00e4nnlich \u2013 Testosteron-definiert<\/a><\/li>\n\n<\/ul>\n<p style=\"margin:14px 0 0\"><a href=\"https:\/\/seamlessbio.de\/de\/request-quote\/\" style=\"font-weight:600\">Angebot anfordern \u2192<\/a><\/p>\n<\/div>\n<!-- \/BUY BOX -->\n\n<\/div>\n\n<!-- SB-BUY-BOX --><div style=\"border:1px solid #daf3f2;border-left:4px solid #18b7b2;background:#f1faf9;border-radius:0 10px 10px 0;padding:14px 18px;margin:18px 0 26px;font-size:15px;line-height:1.6\"><strong>Bereit zu bestellen?<\/strong> <a href=\"https:\/\/seamlessbio.de\/de\/fks-hitzeinaktiviert\/\" style=\"color:#18b7b2;font-weight:700\">Hitzeinaktiviertes FKS kaufen \u2192<\/a><br><span style=\"font-size:14px;color:#5b6b71\">EU-Lager \u00b7 Testmuster auf Anfrage \u00b7 keine Mindestmenge \u00b7 Chargenreservierung bis 36 Monate<\/span><\/div>\n<h2>Was entfernt die Hitzeinaktivierung?<\/h2>\n<p>Die Hitzeinaktivierung zerst\u00f6rt gezielt das Komplementsystem \u2013 eine Kaskade aus ~30 Serumproteinen, die gemeinsam fremde Zellen \u00fcber den Membranangriffskomplex (MAC) lysieren. In der Zellkultur kann bovines Komplement humane Zellen (besonders Lymphozyten und andere Immunzellen) unspezifisch lysieren oder Virusneutralisationsassays st\u00f6ren, in denen eine komplementabh\u00e4ngige Neutralisation zur antik\u00f6rperabh\u00e4ngigen hinzuk\u00e4me.<\/p>\n\n<h2>Wann wird HI-FKS ben\u00f6tigt?<\/h2>\n<ul>\n  <li><strong>Embryokultur (IVF):<\/strong> HI-FKS mit 5\u201310 % ist Standard f\u00fcr die Embryokultur bei Rind, Pferd und Schwein \u2013 Komplementaktivit\u00e4t kann Blastomeren sch\u00e4digen<\/li>\n  <li><strong>PBMC- und Immunzellassays:<\/strong> Humane Lymphozyten reagieren empfindlich auf Lyse durch bovines Komplement \u2013 HI-FKS verwenden oder auf humanes AB-Serum umsteigen<\/li>\n  <li><strong>Virusneutralisationsassays:<\/strong> Verhindert, dass eine komplementabh\u00e4ngige Neutralisation die antik\u00f6rpervermittelte Neutralisation verf\u00e4lscht<\/li>\n  <li><strong>Mykoplasmenfreie Kultur:<\/strong> Hitzeinaktivierung t\u00f6tet Mykoplasmen NICHT ab \u2013 stattdessen PCR-getestetes FKS verwenden<\/li>\n<\/ul>\n\n<h2>Wann wird HI-FKS NICHT ben\u00f6tigt?<\/h2>\n<p>F\u00fcr die routinem\u00e4\u00dfige Kultur von S\u00e4ugerzelllinien (HeLa, A549, CHO, HEK293, MCF-7) ist die Hitzeinaktivierung unn\u00f6tig und senkt die wachstumsf\u00f6rdernde Wirkung leicht. F\u00fcr die allgemeine Zellkultur ist Standard-FKS vorzuziehen.<\/p>\n\n<h2>Wichtige Fakten<\/h2>\n<ul>\n<li>Die Hitzeinaktivierung erfolgt bei genau 56 \u00b0C f\u00fcr 30 Minuten \u2013 h\u00f6here Temperaturen oder l\u00e4ngere Zeiten denaturieren Albumin und Wachstumsfaktoren und senken die biologische Aktivit\u00e4t<\/li>\n<li>Hauptziel sind die Komplementproteine (C1q, C3, C4, C5) \u2013 die klassische Komplementkaskade ist hitzelabil und wird bei 56 \u00b0C inaktiviert<\/li>\n<li>Die Hitzeinaktivierung entfernt NICHT: Endotoxin, IgG, Mykoplasmen, Viren, Wachstumsfaktoren (geringe Abnahme) oder Albumin<\/li>\n<li>HI-FKS ist Standard f\u00fcr: Embryokultur (IVF bei Rind, Pferd, Schwein), PBMC-Assays, Virusneutralisationsassays und jede Anwendung, in der eine komplementvermittelte Lyse der Zielzellen die Ergebnisse verf\u00e4lschen w\u00fcrde<\/li>\n<li>Die Hitzeinaktivierung senkt die wachstumsf\u00f6rdernde Wirkung leicht \u2013 manche Zelllinien wachsen in HI-FKS langsamer als in Standard-FKS<\/li>\n<li>F\u00fcr die meisten Routinekulturen nicht n\u00f6tig \u2013 nur einsetzen, wenn Komplementaktivit\u00e4t f\u00fcr Ihren Assay nachweislich ein Problem ist<\/li>\n<\/ul>\n\n<h2>Weiterlesen<\/h2>\n<ul>\n<li><a href=\"https:\/\/seamlessbio.de\/de\/what-is-fbs\/\">Was ist FBS?<\/a><\/li>\n<li><a href=\"https:\/\/seamlessbio.de\/de\/fortpflanzung-bei-tieren\/\">Leitfaden: Seren f\u00fcr Tierreproduktion &amp; IVF<\/a><\/li>\n<li><a href=\"https:\/\/seamlessbio.de\/de\/fetales-kaelberserum\/\">FKS-Portfolio \u2013 alle Qualit\u00e4ten<\/a><\/li>\n<\/ul>\n\n<div style=\"background:#f4f6f7;border-radius:12px;padding:32px;text-align:center;margin-top:40px;\">\n<p style=\"font-size:18px;font-weight:700;color:#1a2e35;margin-bottom:12px;\">FKS hitzeinaktiviert \u2013 erh\u00e4ltlich bei SeamlessBio<\/p>\n<p style=\"color:#6b7f82;margin-bottom:20px;\">Muster oder Angebot innerhalb von 48 Stunden anfordern.<\/p>\n<a href=\"https:\/\/seamlessbio.de\/de\/fks-hitzeinaktiviert\/\" style=\"background:#18b7b2;color:#fff;padding:12px 28px;border-radius:50px;font-size:13px;font-weight:700;text-decoration:none;\">Zum Produkt \u2192<\/a>\n&nbsp;&nbsp;\n<a href=\"https:\/\/seamlessbio.de\/de\/kontakt\/\" style=\"border:2px solid #18b7b2;color:#139b97;padding:10px 28px;border-radius:50px;font-size:13px;font-weight:700;text-decoration:none;\">Muster anfordern<\/a>\n<\/div>\n\n<p style=\"font-size:13px;color:#6b7f82;margin-top:32px;border-top:1px solid #d8e8e7;padding-top:16px;\">\n<em>SeamlessBio \u00b7 Passau, Deutschland \u00b7 <a href=\"mailto:info@seamlessbio.de\">info@seamlessbio.de<\/a> \u00b7 +49 851 37932226<\/em>\n<\/p>\t\t\t\t<\/div>\n\t\t\n<\/div>\n\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Quick Definition What is Heat Inactivated FBS?: Heat inactivated FBS (HI-FBS) is fetal bovine serum that has been heated to 56\u00b0C for 30 minutes to destroy complement proteins. It is used in cell culture applications where active complement activity would lyse cells or interfere with assay results. Buy fetal bovine serum (FBS) from EU stock [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_templately_pack_id":"","_templately_imported_at":"","_templately_source":"","_templately_import_session_id":"","footnotes":""},"class_list":["post-2888","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Heat Inactivated FBS (HI FBS): What It Is &amp; When to Use<\/title>\n<meta name=\"description\" content=\"Heat inactivated FBS explained: what 56 \u00b0C for 30 min removes, HI FBS vs regular FBS, and when you really need it. Lot-tested HI FBS from EU stock.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/seamlessbio.de\/de\/what-is-heat-inactivated-fbs\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Heat Inactivated FBS (HI FBS): What It Is &amp; When to Use\" \/>\n<meta property=\"og:description\" content=\"Heat inactivated FBS explained: what 56 \u00b0C for 30 min removes, HI FBS vs regular FBS, and when you really need it. 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