{"id":2898,"date":"2026-08-22T15:47:48","date_gmt":"2026-08-22T14:47:48","guid":{"rendered":"https:\/\/seamlessbio.de\/?page_id=2898"},"modified":"2026-08-23T00:58:46","modified_gmt":"2026-08-22T23:58:46","slug":"what-is-human-platelet-lysate-hpl","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/what-is-human-platelet-lysate-hpl\/","title":{"rendered":"What is Human Platelet Lysate (hPL)"},"content":{"rendered":"
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Quick Definition<\/p>\n

What is Human Platelet Lysate (hPL)?:<\/strong> Human platelet lysate (hPL) is a human-derived cell culture supplement produced by freeze-thaw lysis of human platelets. It releases a concentrated pool of platelet-derived growth factors (PDGF, TGF-\u03b2, EGF, VEGF, FGF-2, IGF-1) into plasma and is used as a xeno-free, GMP-compatible alternative to FBS for human cell culture \u2014 particularly MSC expansion.<\/p>\n<\/div>\n\n

How is hPL made?<\/h2>\n

Platelets are collected from screened human donors via apheresis or whole blood donation. The platelet concentrate is subjected to 3\u20135 freeze-thaw cycles (typically \u221280\u00b0C \u2192 37\u00b0C), which ruptures platelet cell membranes and releases intracellular contents \u2014 including dense granules (ADP, serotonin, calcium), alpha granules (PDGF, TGF-\u03b2, EGF, vWF, fibronectin), and lysosomal contents \u2014 into the surrounding plasma. The lysate is then filtered (0.2 \u00b5m) and pooled from multiple donors to reduce lot-to-lot variability.<\/p>\n\n

hPL vs FBS \u2014 key differences<\/h2>\n

Unlike FBS, hPL provides human-sequence growth factors that are species-matched for human cell culture. For MSCs, fibroblasts, endothelial cells and other primary human cells, human growth factor receptor binding is more efficient with human-sequence ligands than with bovine FBS equivalents. This explains why hPL at 5% often achieves higher proliferation rates than FBS at 10% for human cell types.<\/p>\n\n

When to use hPL instead of FBS<\/h2>\n

Use hPL for: MSC expansion (bone marrow, adipose, umbilical cord), primary human fibroblasts, endothelial cells, keratinocytes, CAR-T and NK cell manufacturing, and any xeno-free ATMP protocol. Keep FBS for: rodent cell lines, hybridoma culture, standard cancer cell lines, and viral vector production.<\/p>\n\n

Key Facts<\/h2>\n