{"id":2903,"date":"2026-08-22T15:48:36","date_gmt":"2026-08-22T14:48:36","guid":{"rendered":"https:\/\/wheat-quail-856400.hostingersite.com\/?page_id=2903"},"modified":"2026-11-12T18:44:42","modified_gmt":"2026-11-12T17:44:42","slug":"what-is-off-the-clot-serum","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/what-is-off-the-clot-serum\/","title":{"rendered":"Was ist Off-the-Clot (OTC)-Serum?"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"2903\" class=\"elementor elementor-2903\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-02047c6 e-con e-atomic-element e-flexbox-base e-default-div e-522b368\" data-id=\"02047c6\" data-element_type=\"e-flexbox\" data-e-type=\"e-flexbox\" data-interaction-id=\"02047c6\">\n    \t\t<div class=\"elementor-element elementor-element-4dd4d1f elementor-widget elementor-widget-html\" data-id=\"4dd4d1f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t\t<!-- \nWORDPRESS PAGE \u2014 PASTE INTO GUTENBERG \"BENUTZERDEFINIERTES HTML\" BLOCK\nYoast SEO:\n  Slug: what-is-off-the-clot-serum\n  SEO Title: What is Off-the-Clot (OTC) Serum? \u2014 Definition & Uses | SeamlessBio\n  Meta Description: What is off-the-clot serum, how it differs from standard plasma-derived serum, why it has more growth factors, and when to use it. Definition from SeamlessBio.\n  Focus Keyword: what is off-the-clot serum\n  Meta Robots: index, follow\n  Page Type: Article\n-->\n\n<div style=\"background:#e9fbfa;border-left:4px solid #18b7b2;border-radius:0 8px 8px 0;padding:20px 24px;margin-bottom:32px;\">\n<p style=\"font-size:11px;font-weight:700;text-transform:uppercase;letter-spacing:1px;color:#139b97;margin-bottom:8px;\">Kurzdefinition<\/p><p class=\"sb-hs-buy\" style=\"background:#f1faf9;border-left:4px solid #18b7b2;border-radius:8px;padding:12px 16px;margin:16px 0\">Sie m\u00f6chten kaufen? Alle Qualit\u00e4ten finden Sie unter <a href=\"https:\/\/seamlessbio.de\/de\/humanserum\/\">Humanserum<\/a> : gemischte Spender, Typ AB, Off-the-Clot, m\u00e4nnlich \u2013 EU-Lager, Chargenreservierung bis 36 Monate.<\/p>\n<p style=\"font-size:16px;margin:0;\"><strong>Was ist Off-the-Clot-Serum (OTC)?:<\/strong> Off-the-Clot-Serum (OTC) ist Humanserum, das entsteht, wenn Vollblut ohne Antikoagulans bei Raumtemperatur auf nat\u00fcrliche Weise gerinnt. Dabei werden die Thrombozyten vollst\u00e4ndig aktiviert und degranulieren, sodass Wachstumsfaktoren (PDGF, EGF, TGF-\u03b2) ins Serum gelangen. OTC-Serum enth\u00e4lt daher mehr Wachstumsfaktoren als plasmagewonnenes Standardserum.<\/p>\n<\/div>\n\n<h2>OTC-Serum vs. Standardserum \u2013 was ist der Unterschied?<\/h2>\n<p>Standard-Humanserum wird aus Plasma hergestellt, das mit Antikoagulans gewonnen wurde \u2013 die Gerinnung wird anschlie\u00dfend k\u00fcnstlich mit Calciumchlorid ausgel\u00f6st. Die Thrombozyten bleiben weitgehend intakt und degranulieren nicht vollst\u00e4ndig, sodass ihre Wachstumsfaktoren eingeschlossen bleiben. Das Ergebnis ist ein Serum mit kontrollierterem, reproduzierbarem Proteinprofil, aber geringerer mitogener Aktivit\u00e4t.<\/p>\n<p>Im OTC-Serum f\u00fchrt die nat\u00fcrliche Gerinnung zur vollst\u00e4ndigen Thrombozytenaktivierung. Alle intrazellul\u00e4ren Wachstumsfaktoren werden freigesetzt und ergeben ein deutlich reicheres Wachstumsfaktorumfeld \u2013 besser f\u00fcr die Zellproliferation, schlechter f\u00fcr die Assaykonsistenz.<\/p>\n\n<h2>Wichtige Anwendungen f\u00fcr OTC-Serum<\/h2>\n<ul>\n  <li><strong>Malariakultur (P. falciparum):<\/strong> erfordert humanes AB-OTC-Serum \u2013 nicht durch FKS oder Standardserum ersetzbar<\/li>\n  <li><strong>Hybridomklonierung:<\/strong> PDGF aus der Thrombozytendegranulation unterst\u00fctzt die Koloniebildung von Hybridomen aus B-Lymphozyten<\/li>\n  <li><strong>Humane Prim\u00e4rzellkultur:<\/strong> mehr EGF und PDGF verbessern die Proliferation von Fibroblasten, Keratinozyten und Endothelzellen<\/li>\n  <li><strong>Migration im Scratch-Assay:<\/strong> h\u00f6here chemotaktische Aktivit\u00e4t f\u00fcr Wundheilungsassays<\/li>\n<\/ul>\n\n<h2>Wichtige Fakten<\/h2>\n<ul>\n<li>Das Blut wird ohne Antikoagulans entnommen und gerinnt 30\u201360 Minuten auf nat\u00fcrliche Weise bei Raumtemperatur<\/li>\n<li>Die nat\u00fcrliche Gerinnung aktiviert die Thrombozyten \u00fcber Thrombin \u2013 die vollst\u00e4ndige Degranulation setzt PDGF-AB\/BB, EGF, TGF-\u03b21, VEGF, FGF-2 und Serotonin frei<\/li>\n<li>Der Wachstumsfaktorgehalt ist deutlich h\u00f6her als bei (plasmagewonnenem) Standardserum aus demselben Spenderpool<\/li>\n<li>OTC-Serum Typ AB ist der Standard f\u00fcr die Malariakultur von Plasmodium falciparum \u2013 eine der wenigen Anwendungen, in denen OTC die einzige brauchbare Option ist<\/li>\n<li>Eingesetzt in der Hybridomkultur und der humanen Prim\u00e4rzellkultur, wenn maximale mitogene Aktivit\u00e4t gefragt ist<\/li>\n<li>NICHT empfohlen f\u00fcr IVD-Kalibratoren, Zytokin-Multiplexassays oder DMPK-Proteinbindungsassays \u2013 h\u00f6here Variabilit\u00e4t und TGF-\u03b2-Hintergrund<\/li>\n<li>Erh\u00e4ltlich bei SeamlessBio: humanes OTC-Serum AB, OTC gemischt \u2013 Spender aus EU und USA, nativ und hitzeinaktiviert<\/li>\n<\/ul>\n\n<h2>Weiterlesen<\/h2>\n<ul>\n<li><a href=\"https:\/\/seamlessbio.de\/de\/off-the-clot-serum-explained\/\">Off-the-Clot-Serum erkl\u00e4rt \u2013 vollst\u00e4ndiger Leitfaden<\/a><\/li>\n<li><a href=\"https:\/\/seamlessbio.de\/de\/what-is-human-ab-serum\/\">Was ist Human Serum AB?<\/a><\/li>\n<li><a href=\"https:\/\/seamlessbio.de\/de\/humanseren-plasma\/\">Portfolio Humanserum<\/a><\/li>\n<\/ul>\n\n<div style=\"background:#f4f6f7;border-radius:12px;padding:32px;text-align:center;margin-top:40px;\">\n<p style=\"font-size:18px;font-weight:700;color:#1a2e35;margin-bottom:12px;\">Humanes OTC-Serum \u2013 erh\u00e4ltlich bei SeamlessBio<\/p>\n<p style=\"color:#6b7f82;margin-bottom:20px;\">Muster oder Angebot innerhalb von 48 Stunden anfordern.<\/p>\n<a href=\"https:\/\/seamlessbio.de\/de\/humanserum-otc\/\" style=\"background:#18b7b2;color:#fff;padding:12px 28px;border-radius:50px;font-size:13px;font-weight:700;text-decoration:none;\">Zum Produkt \u2192<\/a>\n&nbsp;&nbsp;\n<a href=\"https:\/\/seamlessbio.de\/de\/kontakt\/\" style=\"border:2px solid #18b7b2;color:#139b97;padding:10px 28px;border-radius:50px;font-size:13px;font-weight:700;text-decoration:none;\">Muster anfordern<\/a>\n<\/div>\n\n<p style=\"font-size:13px;color:#6b7f82;margin-top:32px;border-top:1px solid #d8e8e7;padding-top:16px;\">\n<em>SeamlessBio \u00b7 Passau, Deutschland \u00b7 <a href=\"mailto:info@seamlessbio.de\">info@seamlessbio.de<\/a> \u00b7 +49 851 37932226<\/em>\n<\/p>\t\t\t\t<\/div>\n\t\t\n<\/div>\n\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Quick Definition Looking to buy? See our human serum overview \u2013 all grades (mixed donors, Type AB, off-the-clot, male-only), EU stock, lot reservation up to 36 months. What is Off-the-Clot (OTC) Serum?: Off-the-clot (OTC) serum is human serum produced by allowing whole blood to clot naturally at room temperature without anticoagulant. During natural clotting, platelets [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_templately_pack_id":"","_templately_imported_at":"","_templately_source":"","_templately_import_session_id":"","footnotes":""},"class_list":["post-2903","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>What is Off-the-Clot (OTC) Serum? \u2014 Definition &amp; Uses<\/title>\n<meta name=\"description\" content=\"What is off-the-clot serum, how it differs from standard plasma-derived serum, why it has more growth factors, and when to use it. Definition from SeamlessBio.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/seamlessbio.de\/de\/what-is-off-the-clot-serum\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"What is Off-the-Clot (OTC) Serum? \u2014 Definition &amp; Uses\" \/>\n<meta property=\"og:description\" content=\"What is off-the-clot serum, how it differs from standard plasma-derived serum, why it has more growth factors, and when to use it. 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