{"id":2998,"date":"2026-08-22T21:06:51","date_gmt":"2026-08-22T20:06:51","guid":{"rendered":"https:\/\/wheat-quail-856400.hostingersite.com\/?page_id=2998"},"modified":"2026-11-12T18:43:58","modified_gmt":"2026-11-12T17:43:58","slug":"what-is-normal-donkey-serum","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/what-is-normal-donkey-serum\/","title":{"rendered":"Was ist normales Eselserum?"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"2998\" class=\"elementor elementor-2998\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b603f9e e-con e-atomic-element e-flexbox-base e-default-div e-908aa8b\" data-id=\"b603f9e\" data-element_type=\"e-flexbox\" data-e-type=\"e-flexbox\" data-interaction-id=\"b603f9e\">\n    \t\t<div class=\"elementor-element elementor-element-0daf13a elementor-widget elementor-widget-html\" data-id=\"0daf13a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t\t<!-- \nWORDPRESS PAGE \u2014 GUTENBERG \"BENUTZERDEFINIERTES HTML\" BLOCK\nSlug: what-is-normal-donkey-serum\nSEO Title: What is Normal Donkey Serum (NDS)? \u2014 IHC Blocking Guide | SeamlessBio\nMeta Description: What is normal donkey serum (NDS), why is it the most common IHC blocking serum, and when is it required over goat or rabbit serum?\nFocus Keyword: what is normal donkey serum NDS IHC blocking\nAdditional Keywords: normal donkey serum blocking immunofluorescence, NDS IHC blocking protocol, donkey serum secondary antibody\nMenu: Resources \u2192 Glossary\n-->\n<div style=\"background:#e9fbfa;border-left:4px solid #18b7b2;border-radius:0 8px 8px 0;padding:20px 24px;margin-bottom:32px;\">\n<p style=\"font-size:11px;font-weight:700;text-transform:uppercase;letter-spacing:1px;color:#139b97;margin-bottom:8px;\">Kurzdefinition<\/p>\n<p style=\"font-size:16px;margin:0;\"><strong>Was ist normales Eselserum (NDS)?<\/strong> Normales Eselserum (NDS) ist Serum gesunder Esel, das in IHC und IF als Blockierreagenz dient, wenn die Sekund\u00e4rantik\u00f6rper im Esel hergestellt wurden. Es ist das am h\u00e4ufigsten verwendete Blockierserum bei Mehrfachmarkierungen in der Immunfluoreszenz, weil die meisten kommerziellen kreuzadsorbierten Sekund\u00e4rantik\u00f6rper-Panels im Esel hergestellt werden.<\/p>\n<!-- BUY BOX (SEO: links to buy pages) -->\n<div class=\"sb-buy-box\" style=\"border:1px solid #cfe9e6;background:#f4fbfa;border-radius:12px;padding:22px 26px;margin:28px auto;max-width:1200px;box-sizing:border-box\">\n<h2 style=\"font-size:22px;line-height:1.3;margin:0 0 6px\">Tierseren ab EU-Lager kaufen<\/h2>\n<p style=\"margin:0 0 14px\">Angebot anfragen \u2013 EU-Lager, Chargendokumentation, Chargenreservierung bis zu 36 Monate.<\/p>\n<ul style=\"margin:0;padding-left:20px;columns:2 300px;column-gap:32px\">\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/eselserum-eu-sterilfiltriert\/\" style=\"font-weight:700\">Normales Eselserum<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/neugeborenen-kaelberserum-ncs-usa\/\">Neugeborenen-K\u00e4lberserum (NCS) \u2013 Herkunft USA<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/neugeborenen-kaelberserum-ncs-australien\/\">Neugeborenen-K\u00e4lberserum (NCS) \u2013 Herkunft Australien<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/kaelberserum-cs-usa-sterilfiltriert\/\">K\u00e4lberserum (CS) \u2013 Herkunft USA<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/kaelberserum-cs-australien-sterilfiltriert\/\">K\u00e4lberserum (CS) \u2013 Herkunft Australien<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/pferdeserum-eu-sterilfiltriert\/\">Pferdeserum \u2013 EU-Herkunft<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/spender-pferdeserum-dhs-eu\/\">Spenderpferdeserum (DHS)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/ziegenserum-normal-ngs-eu\/\">Normales Ziegenserum (NGS)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/kaninchenserum-normal-nrs-eu\/\">Normales Kaninchenserum (NRS)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/meerschweinchenserum-komplement\/\">Meerschweinchenserum \u2013 Komplementquelle<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/mausserum-normal-nms-eu\/\">Normales Mausserum<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/rattenserum-eu-sterilfiltriert\/\">Ratten-Serum<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkte\/seren-anderer-tiere\/\">Alle Tierseren \u2013 16 Tierarten<\/a><\/li>\n<\/ul>\n<p style=\"margin:14px 0 0\"><a href=\"https:\/\/seamlessbio.de\/de\/request-quote\/\" style=\"font-weight:600\">Angebot anfordern \u2192<\/a><\/p>\n<\/div>\n<!-- \/BUY BOX -->\n\n<\/div>\n<!-- SB-BUY-BOX --><div style=\"border:1px solid #daf3f2;border-left:4px solid #18b7b2;background:#f1faf9;border-radius:0 10px 10px 0;padding:14px 18px;margin:18px 0 26px;font-size:15px;line-height:1.6\"><strong>Bereit zu bestellen?<\/strong> <a href=\"https:\/\/seamlessbio.de\/de\/eselserum\/\" style=\"color:#18b7b2;font-weight:700\">Normales Eselserum (NDS) kaufen \u2192<\/a><br><span style=\"font-size:14px;color:#5b6b71\">EU-Lager \u00b7 Testmuster auf Anfrage \u00b7 keine Mindestmenge \u00b7 Chargenreservierung bis 36 Monate<\/span><\/div>\n<h2>Warum Eselserum zum Standard f\u00fcr Mehrfach-IF wurde<\/h2>\n<p>Werden mehrere Prim\u00e4rantik\u00f6rper aus verschiedenen Wirtsspezies gleichzeitig eingesetzt (z. B. Maus-Anti-CD3 + Kaninchen-Anti-Ki67 + Ziege-Anti-CK5), muss jeder Sekund\u00e4rantik\u00f6rper eine andere Prim\u00e4rspezies erkennen, ohne mit den anderen zu kreuzreagieren. Im Esel hergestellte Sekund\u00e4rantik\u00f6rper (Esel-Anti-Maus, Esel-Anti-Kaninchen, Esel-Anti-Ziege) gibt es gegen alle g\u00e4ngigen Spezies vorabsorbiert \u2013 damit deckt der Esel als einzige Spezies alle Prim\u00e4rwirte mit einem NDS-Blockierschritt ab.<\/p>\n<h2>NDS-Blockierprotokoll<\/h2>\n<ol>\n<li>Nach der Antigendemaskierung \u00fcbersch\u00fcssigen Puffer abtropfen lassen<\/li>\n<li>5\u201310 % NDS in PBS auftragen, 200\u2013300 \u00b5l pro Schnitt<\/li>\n<li>30\u201360 min bei Raumtemperatur inkubieren<\/li>\n<li>Abtropfen lassen (nicht waschen) \u2013 Prim\u00e4rantik\u00f6rper in 1\u20135 % NDS\/PBS auftragen<\/li>\n<\/ol>\n<h2>Wichtige Fakten<\/h2>\n<ul>\n<li>Spezies-Regel: Die Spezies des Blockierserums muss der Spezies entsprechen, in der der Sekund\u00e4rantik\u00f6rper hergestellt wurde<\/li>\n<li>Ist der Sekund\u00e4rantik\u00f6rper Esel-Anti-Kaninchen-AF488 \u2192 Blockierserum muss normales Eselserum (NDS) sein<\/li>\n<li>NDS ist der Standard f\u00fcr Mehrfach-IF, weil die meisten kommerziellen kreuzadsorbierten Sekund\u00e4rantik\u00f6rper im Esel hergestellt werden<\/li>\n<li>Bei Mehrfachmarkierung mit 3\u20134 Prim\u00e4rantik\u00f6rpern: Sekund\u00e4rantik\u00f6rper einer Spezies (Esel) + ein NDS-Blockierschritt \u2013 das einfachste Protokoll<\/li>\n<li>Empfohlen: 5\u201310 % NDS in PBS, 30\u201360 Minuten bei Raumtemperatur, vor dem Prim\u00e4rantik\u00f6rper nicht waschen<\/li>\n<li>Erh\u00e4ltlich bei SeamlessBio: 50 ml, 100 ml, 500 ml; hitzeinaktiviert auf Anfrage<\/li>\n<\/ul>\n<h2>Weiterlesen<\/h2>\n<ul>\n<li><a href=\"https:\/\/seamlessbio.de\/de\/ressourcen\/blog\/normal-serum-ihc-if-blocking-guide\/\">Leitfaden: Normalserum zum Blocken in IHC\/IF<\/a><\/li>\n<li><a href=\"https:\/\/seamlessbio.de\/de\/what-is-horse-serum\/\">Was ist Pferdeserum?<\/a><\/li>\n<li><a href=\"https:\/\/seamlessbio.de\/de\/produkte\/animal-sera\/\">Tierseren-Portfolio<\/a><\/li>\n<\/ul>\n<div style=\"background:#f4f6f7;border-radius:12px;padding:32px;text-align:center;margin-top:40px;\">\n<p style=\"font-size:18px;font-weight:700;color:#1a2e35;margin-bottom:12px;\">Normales Eselserum (NDS) \u2013 erh\u00e4ltlich bei SeamlessBio<\/p>\n<a href=\"https:\/\/seamlessbio.de\/de\/produkte\/animal-sera\/\" style=\"background:#18b7b2;color:#fff;padding:12px 28px;border-radius:50px;font-size:13px;font-weight:700;text-decoration:none;\">Zum Produkt \u2192<\/a>\n&nbsp;&nbsp;\n<a href=\"https:\/\/seamlessbio.de\/de\/kontakt\/\" style=\"border:2px solid #18b7b2;color:#139b97;padding:10px 28px;border-radius:50px;font-size:13px;font-weight:700;text-decoration:none;\">Muster anfordern<\/a>\n<\/div>\n<p style=\"font-size:13px;color:#6b7f82;margin-top:32px;border-top:1px solid #d8e8e7;padding-top:16px;\">\n<em>SeamlessBio \u00b7 Passau, Deutschland \u00b7 <a href=\"mailto:info@seamlessbio.de\">info@seamlessbio.de<\/a><\/em>\n<\/p>\t\t\t\t<\/div>\n\t\t\n<\/div>\n\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Quick Definition What is Normal Donkey Serum (NDS)?: Normal Donkey Serum (NDS) is serum from healthy donkeys used as a blocking reagent in IHC and IF when secondary antibodies are raised in donkey. It has become the most widely used blocking serum in multi-label immunofluorescence because most commercial cross-adsorbed secondary antibody panels are donkey-raised. Buy [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_templately_pack_id":"","_templately_imported_at":"","_templately_source":"","_templately_import_session_id":"","footnotes":""},"class_list":["post-2998","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Normal Donkey Serum (NDS) for Blocking \u2013 IHC\/IF Guide<\/title>\n<meta name=\"description\" content=\"Normal donkey serum explained: why NDS is the standard blocking serum with donkey secondary antibodies, how it compares to goat serum, and EU sourcing.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/seamlessbio.de\/de\/what-is-normal-donkey-serum\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Normal Donkey Serum (NDS) for Blocking \u2013 IHC\/IF Guide\" \/>\n<meta property=\"og:description\" content=\"Normal donkey serum explained: why NDS is the standard blocking serum with donkey secondary antibodies, how it compares to goat serum, and EU sourcing.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/seamlessbio.de\/de\/what-is-normal-donkey-serum\/\" \/>\n<meta property=\"og:site_name\" content=\"SeamlessBio\" \/>\n<meta property=\"article:modified_time\" content=\"2026-11-12T17:43:58+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/seamlessbio.de\/wp-content\/uploads\/2026\/04\/134428046.png\" \/>\n\t<meta property=\"og:image:width\" content=\"973\" \/>\n\t<meta property=\"og:image:height\" content=\"288\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Gesch\u00e4tzte Lesezeit\" \/>\n\t<meta name=\"twitter:data1\" content=\"2\u00a0Minuten\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/seamlessbio.de\\\/what-is-normal-donkey-serum\\\/\",\"url\":\"https:\\\/\\\/seamlessbio.de\\\/what-is-normal-donkey-serum\\\/\",\"name\":\"Normal Donkey Serum (NDS) for Blocking \u2013 IHC\\\/IF Guide\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/seamlessbio.de\\\/#website\"},\"datePublished\":\"2026-08-22T20:06:51+00:00\",\"dateModified\":\"2026-11-12T17:43:58+00:00\",\"description\":\"Normal donkey serum explained: why NDS is the standard blocking serum with donkey secondary antibodies, how it compares to goat serum, and EU sourcing.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/seamlessbio.de\\\/what-is-normal-donkey-serum\\\/#breadcrumb\"},\"inLanguage\":\"de\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/seamlessbio.de\\\/what-is-normal-donkey-serum\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/seamlessbio.de\\\/what-is-normal-donkey-serum\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Start\",\"item\":\"https:\\\/\\\/seamlessbio.de\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"What is Normal Donkey Serum (NDS)?\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/seamlessbio.de\\\/#website\",\"url\":\"https:\\\/\\\/seamlessbio.de\\\/\",\"name\":\"SeamlessBio\",\"description\":\"Human serum, plasma and hPL, single-use bioprocessing bags, serum-free media, animal sera and FBS. 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