{"id":3003,"date":"2026-08-22T21:07:28","date_gmt":"2026-08-22T20:07:28","guid":{"rendered":"https:\/\/seamlessbio.de\/?page_id=3003"},"modified":"2026-08-23T00:26:55","modified_gmt":"2026-08-22T23:26:55","slug":"what-is-guinea-pig-complement","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/what-is-guinea-pig-complement\/","title":{"rendered":"What is Guinea Pig Complement?"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"3003\" class=\"elementor elementor-3003\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-d5bb22c e-con e-atomic-element e-flexbox-base e-32b3486\" data-id=\"d5bb22c\" data-element_type=\"e-flexbox\" data-e-type=\"e-flexbox\" data-interaction-id=\"d5bb22c\">\n    \t\t<div class=\"elementor-element elementor-element-da6b3c0 elementor-widget elementor-widget-html\" data-id=\"da6b3c0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t\t<!-- \nWORDPRESS PAGE \u2014 GUTENBERG \"BENUTZERDEFINIERTES HTML\" BLOCK\nSlug: what-is-guinea-pig-complement\nSEO Title: What is Guinea Pig Complement? \u2014 Definition & Hemolytic Assay | SeamlessBio\nMeta Description: What is guinea pig complement, why is it used in hemolytic assays and complement fixation tests, and how does source affect activity?\nFocus Keyword: what is guinea pig complement hemolytic assay\nAdditional Keywords: guinea pig complement hemolytic assay, guinea pig serum complement activity, complement fixation test guinea pig\nMenu: Resources \u2192 Glossary\n-->\n<div style=\"background:#e9fbfa;border-left:4px solid #18b7b2;border-radius:0 8px 8px 0;padding:20px 24px;margin-bottom:32px;\">\n<p style=\"font-size:11px;font-weight:700;text-transform:uppercase;letter-spacing:1px;color:#139b97;margin-bottom:8px;\">Quick Definition<\/p>\n<p style=\"font-size:16px;margin:0;\"><strong>What is Guinea Pig Complement?:<\/strong> Guinea pig complement refers to the complement system proteins in guinea pig serum, which has unusually high and consistent complement activity compared to other species. Guinea pig serum is the standard complement source in haemolytic complement assays (CH50), complement fixation tests (CFT), and any assay requiring active, potent complement activity that can be titrated reproducibly.<\/p>\n<\/div>\n<h2>Why guinea pig serum for complement assays?<\/h2>\n<p>Guinea pig serum has been the standard complement source since the early 20th century because it consistently provides high-titre complement activity that can be titrated to a defined CH50 per lot \u2014 essential for complement fixation tests used in serology laboratories.<\/p>\n<h2>Critical handling requirements<\/h2>\n<p>Guinea pig complement must never be heat inactivated \u2014 the complement proteins are the entire point. Keep frozen at \u221270\u00b0C until use. Thaw rapidly in a 37\u00b0C water bath, immediately place on ice. Use within 2 hours. Never refreeze. Titrate each lot to determine the exact volume for one CH50 unit in your specific assay format before quantitative measurements.<\/p>\n<h2>Key Facts<\/h2>\n<ul>\n<li>Guinea pig serum has the highest and most consistent complement activity of any commonly available animal serum<\/li>\n<li>Used as exogenous complement source in: haemolytic complement assays (CH50, AH50), complement fixation test (CFT), bactericidal assays, opsonophagocytosis assays<\/li>\n<li>Complement activity measured in CH50 units \u2014 one unit lyses 50% of sensitised sheep red blood cells under defined conditions<\/li>\n<li>Must be stored at \u221270\u00b0C or below \u2014 complement proteins are extremely heat-labile; repeated freeze-thaw destroys activity<\/li>\n<li>Lot-to-lot variability in complement activity is significant \u2014 always titrate new lots before use in quantitative assays<\/li>\n<li>Available from SeamlessBio: 50 mL, 100 mL, 500 mL; non-heat inactivated; frozen shipment required<\/li>\n<\/ul>\n<h2>Further Reading<\/h2>\n<ul>\n<li><a href=\"https:\/\/seamlessbio.de\/de\/produkte\/animal-sera\/\">Animal Sera Portfolio<\/a><\/li>\n<li><a href=\"https:\/\/seamlessbio.de\/de\/ressourcen\/blog\/normal-serum-ihc-if-blocking-guide\/\">Normal Serum for IHC\/IF Blocking<\/a><\/li>\n<\/ul>\n<div style=\"background:#f4f6f7;border-radius:12px;padding:32px;text-align:center;margin-top:40px;\">\n<p style=\"font-size:18px;font-weight:700;color:#1a2e35;margin-bottom:12px;\">Guinea Pig Complement Serum \u2014 Available from SeamlessBio<\/p>\n<a href=\"https:\/\/seamlessbio.de\/de\/produkte\/animal-sera\/\" style=\"background:#18b7b2;color:#fff;padding:12px 28px;border-radius:50px;font-size:13px;font-weight:700;text-decoration:none;\">View Product \u2192<\/a>\n&nbsp;&nbsp;\n<a href=\"https:\/\/seamlessbio.de\/de\/kontakt\/\" style=\"border:2px solid #18b7b2;color:#139b97;padding:10px 28px;border-radius:50px;font-size:13px;font-weight:700;text-decoration:none;\">Muster anfordern<\/a>\n<\/div>\n<p style=\"font-size:13px;color:#6b7f82;margin-top:32px;border-top:1px solid #d8e8e7;padding-top:16px;\">\n<em>SeamlessBio GmbH \u00b7 Passau, Germany \u00b7 <a href=\"mailto:info@seamlessbio.de\">info@seamlessbio.de<\/a><\/em>\n<\/p>\t\t\t\t<\/div>\n\t\t\n<\/div>\n\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Quick Definition What is Guinea Pig Complement?: Guinea pig complement refers to the complement system proteins in guinea pig serum, which has unusually high and consistent complement activity compared to [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_eb_attr":"","footnotes":""},"class_list":["post-3003","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>What is Guinea Pig Complement? 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