{"id":3018,"date":"2026-08-22T21:09:17","date_gmt":"2026-08-22T20:09:17","guid":{"rendered":"https:\/\/seamlessbio.de\/?page_id=3018"},"modified":"2026-08-23T00:22:24","modified_gmt":"2026-08-22T23:22:24","slug":"what-is-fatty-acid-free-bsa","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/what-is-fatty-acid-free-bsa\/","title":{"rendered":"What is Fatty Acid Free BSA?"},"content":{"rendered":"
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Quick Definition<\/p>\n

What is Fatty Acid Free BSA?:<\/strong> Fatty acid free BSA (BSA FAF) is bovine serum albumin processed to remove bound fatty acids, lipids and steroid hormones by charcoal treatment or solvent extraction. Standard BSA contains 0.5\u20132 moles of fatty acids per mole of albumin \u2014 sufficient to activate fatty acid receptors (PPAR, FFAR) and interfere with IVF, lipid metabolism research and DMPK protein binding measurements.<\/p>\n<\/div>\n

Why fatty acid contamination matters in BSA<\/h2>\n

Albumin has multiple fatty acid binding sites (FA1\u2013FA7). When occupied by endogenous fatty acids, two problems arise: (1) fatty acid receptors on cells (PPAR-\u03b1\/\u03b3\/\u03b4, FFAR1-4) are activated non-specifically creating background; (2) albumin drug binding sites are reduced, artificially lowering apparent drug-albumin affinity in protein binding assays.<\/p>\n

FAF-BSA in DMPK fu,mic assays<\/h2>\n

BSA FAF at 0.5\u20132% is added to microsomal metabolic stability assays to reduce non-specific membrane binding of lipophilic compounds, improving IVIVE accuracy. BSA FAF specifically is used \u2014 not standard BSA \u2014 because it does not introduce competing fatty acids at BSA drug binding sites.<\/p>\n

Key Facts<\/h2>\n