{"id":3018,"date":"2026-08-22T21:09:17","date_gmt":"2026-08-22T20:09:17","guid":{"rendered":"https:\/\/wheat-quail-856400.hostingersite.com\/?page_id=3018"},"modified":"2026-11-02T16:25:02","modified_gmt":"2026-11-02T15:25:02","slug":"what-is-fatty-acid-free-bsa","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/what-is-fatty-acid-free-bsa\/","title":{"rendered":"Was ist fetts\u00e4urefreies BSA?"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"3018\" class=\"elementor elementor-3018\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f7f07c3 e-con e-atomic-element e-flexbox-base e-default-div e-102cb76\" data-id=\"f7f07c3\" data-element_type=\"e-flexbox\" data-e-type=\"e-flexbox\" data-interaction-id=\"f7f07c3\">\n    \t\t<div class=\"elementor-element elementor-element-77b3bcc elementor-widget elementor-widget-html\" data-id=\"77b3bcc\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t\t<!-- \nWORDPRESS PAGE \u2014 GUTENBERG \"BENUTZERDEFINIERTES HTML\" BLOCK\nSlug: what-is-fatty-acid-free-bsa\nSEO Title: What is Fatty Acid Free BSA? \u2014 Definition & IVF\/DMPK Uses | SeamlessBio\nMeta Description: What is fatty acid free BSA, how is it made, and when is it required for IVF, PPAR assays, and DMPK microsomal protein binding?\nFocus Keyword: what is fatty acid free BSA\nAdditional Keywords: fatty acid free BSA IVF, BSA FAF PPAR assay, fatty acid free bovine serum albumin fu mic DMPK\nMenu: Resources \u2192 Glossary\n-->\n<div style=\"background:#e9fbfa;border-left:4px solid #18b7b2;border-radius:0 8px 8px 0;padding:20px 24px;margin-bottom:32px;\">\n<p style=\"font-size:11px;font-weight:700;text-transform:uppercase;letter-spacing:1px;color:#139b97;margin-bottom:8px;\">Kurzdefinition<\/p>\n<p style=\"font-size:16px;margin:0;\"><strong>Was ist fetts\u00e4urefreies BSA?:<\/strong> Fetts\u00e4urefreies BSA (BSA FAF) ist bovines Serumalbumin, aus dem gebundene Fetts\u00e4uren, Lipide und Steroidhormone durch Aktivkohlebehandlung oder L\u00f6sungsmittelextraktion entfernt wurden. Standard-BSA enth\u00e4lt 0,5\u20132 Mol Fetts\u00e4uren pro Mol Albumin \u2013 genug, um Fetts\u00e4urerezeptoren (PPAR, FFAR) zu aktivieren und IVF, Lipidstoffwechselforschung und DMPK-Proteinbindungsmessungen zu st\u00f6ren.<\/p>\n<!-- BUY BOX (SEO: links to buy pages) -->\n<div class=\"sb-buy-box\" style=\"border:1px solid #cfe9e6;background:#f4fbfa;border-radius:12px;padding:22px 26px;margin:28px auto;max-width:1200px;box-sizing:border-box\">\n<h2 style=\"font-size:22px;line-height:1.3;margin:0 0 6px\">Albumin kaufen \u2013 HSA, rHSA und BSA ab EU-Lager<\/h2>\n<p style=\"margin:0 0 14px\">Angebot oder kostenloses Testmuster anfragen \u2013 EU-Lager, Chargenreservierung bis zu 36 Monate.<\/p>\n<ul style=\"margin:0;padding-left:20px;columns:2 300px;column-gap:32px\">\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/bsa-frei-von-fettsauren\/\" style=\"font-weight:700\">BSA Fetts\u00e4urefrei<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/humanes-serumalbumin-hsa-pulver-96\/\">Humanes Serumalbumin (HSA) \u2013 Pulver \u226596 %<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/humanes-serumalbumin-hsa-5-prozent-loesung\/\">HSA-L\u00f6sung 5 %<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/humanes-serumalbumin-hsa-20-prozent-loesung\/\">HSA-L\u00f6sung 20 %<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/humanes-serumalbumin-hsa-25-prozent-loesung\/\">HSA-L\u00f6sung 25 %<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/rhsa-premium\/\">rHSA Premium \u2013 rekombinantes HSA<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/rhsa-economy-rekombinantes-humanes-serumalbumin\/\">rHSA Economy \u2013 rekombinantes HSA<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/bsa-standard-grade-bovines-serumalbumin\/\">BSA Standard Grade \u226596 %<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/bsa-mit-niedrigem-endotoxingehalt\/\">BSA, endotoxinarm<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/?post_type=product&p=3537\">Rekombinantes humanes Transferrin<\/a><\/li>\n<\/ul>\n<p style=\"margin:14px 0 0\"><a href=\"https:\/\/seamlessbio.de\/de\/request-quote\/\" style=\"font-weight:600\">Angebot anfordern \u2192<\/a><\/p>\n<\/div>\n<!-- \/BUY BOX -->\n\n<\/div>\n<h2>Warum Fetts\u00e4uren im BSA ein Problem sind<\/h2>\n<p>Albumin hat mehrere Fetts\u00e4ure-Bindungsstellen (FA1\u2013FA7). Sind sie mit endogenen Fetts\u00e4uren besetzt, entstehen zwei Probleme: (1) Fetts\u00e4urerezeptoren der Zellen (PPAR-\u03b1\/\u03b3\/\u03b4, FFAR1\u20134) werden unspezifisch aktiviert und erzeugen Hintergrund; (2) die Wirkstoffbindungsstellen des Albumins sind reduziert, was die scheinbare Wirkstoff-Albumin-Affinit\u00e4t in Proteinbindungsassays k\u00fcnstlich senkt.<\/p>\n<h2>FAF-BSA in DMPK-fu,mic-Assays<\/h2>\n<p>BSA FAF wird in Konzentrationen von 0,5\u20132 % zu mikrosomalen Stoffwechselstabilit\u00e4tsassays gegeben, um die unspezifische Membranbindung lipophiler Substanzen zu verringern und die IVIVE-Genauigkeit zu verbessern. Verwendet wird gezielt BSA FAF \u2013 nicht Standard-BSA \u2013, weil es keine konkurrierenden Fetts\u00e4uren an die Wirkstoffbindungsstellen bringt.<\/p>\n<h2>Wichtige Fakten<\/h2>\n<ul>\n<li>Standard-BSA: ~0,5\u20132 Mol Fetts\u00e4ure\/Mol Albumin \u2013 \u00fcberwiegend langkettige Fetts\u00e4uren (Palmitat, Oleat, Stearat)<\/li>\n<li>FAF-BSA: &lt;0,005 % Fetts\u00e4uren \u2013 je Charge kolorimetrisch oder per GC-MS best\u00e4tigt<\/li>\n<li>In IVF-Medium mit 3\u20136 mg\/ml eingesetzt \u2013 Fetts\u00e4uren aus Standard-BSA beeintr\u00e4chtigen Spermienmotilit\u00e4t, Kapazitation und Eizellqualit\u00e4t<\/li>\n<li>Eingesetzt in PPAR-, LXR- und FXR-Kernrezeptorassays \u2013 Fetts\u00e4uren in Standard-BSA aktivieren diese Rezeptoren unspezifisch<\/li>\n<li>Eingesetzt zur DMPK-fu,mic-Korrektur \u2013 BSA FAF ist der Branchenstandard, um die Verteilung von Wirkstoffen in mikrosomale Lipide auszugleichen<\/li>\n<li>Eingesetzt als Zusatz in serumfreien Medien, wenn ein definierter Lipidgehalt erforderlich ist<\/li>\n<\/ul>\n<h2>Weiterlesen<\/h2>\n<ul>\n<li><a href=\"https:\/\/seamlessbio.de\/de\/what-is-bsa-bovine-serum-albumin\/\">Was ist BSA?<\/a><\/li>\n<li><a href=\"https:\/\/seamlessbio.de\/de\/ressourcen\/blog\/in-vitro-drug-metabolism-cyp450-microsomes-hepatocytes\/\">In-vitro-Wirkstoffmetabolismus \u2013 Leitfaden zur Matrix<\/a><\/li>\n<li><a href=\"https:\/\/seamlessbio.de\/de\/anwendungen\/fortpflanzung-bei-tieren\/\">Leitfaden Tierreproduktion &amp; IVF<\/a><\/li>\n<\/ul>\n<div style=\"background:#f4f6f7;border-radius:12px;padding:32px;text-align:center;margin-top:40px;\">\n<p style=\"font-size:18px;font-weight:700;color:#1a2e35;margin-bottom:12px;\">BSA fetts\u00e4urefrei \u2013 erh\u00e4ltlich bei SeamlessBio<\/p>\n<a href=\"https:\/\/seamlessbio.de\/de\/produkte\/bsa\/\" style=\"background:#18b7b2;color:#fff;padding:12px 28px;border-radius:50px;font-size:13px;font-weight:700;text-decoration:none;\">Zum Produkt \u2192<\/a>\n&nbsp;&nbsp;\n<a href=\"https:\/\/seamlessbio.de\/de\/kontakt\/\" style=\"border:2px solid #18b7b2;color:#139b97;padding:10px 28px;border-radius:50px;font-size:13px;font-weight:700;text-decoration:none;\">Muster anfordern<\/a>\n<\/div>\n<p style=\"font-size:13px;color:#6b7f82;margin-top:32px;border-top:1px solid #d8e8e7;padding-top:16px;\">\n<em>SeamlessBio \u00b7 Passau, Deutschland \u00b7 <a href=\"mailto:info@seamlessbio.de\">info@seamlessbio.de<\/a><\/em>\n<\/p>\t\t\t\t<\/div>\n\t\t\n<\/div>\n\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Quick Definition What is Fatty Acid Free BSA?: Fatty acid free BSA (BSA FAF) is bovine serum albumin processed to remove bound fatty acids, lipids and steroid hormones by charcoal treatment or solvent extraction. Standard BSA contains 0.5\u20132 moles of fatty acids per mole of albumin \u2014 sufficient to activate fatty acid receptors (PPAR, FFAR) [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_templately_pack_id":"","_templately_imported_at":"","_templately_source":"","_templately_import_session_id":"","footnotes":""},"class_list":["post-3018","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>What is Fatty Acid Free BSA? \u2014 Definition &amp; IVF\/DMPK Uses<\/title>\n<meta name=\"description\" content=\"What is fatty acid free BSA, how is it made, and when is it required for IVF, PPAR assays, and DMPK microsomal protein binding?\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/seamlessbio.de\/de\/what-is-fatty-acid-free-bsa\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"What is Fatty Acid Free BSA? \u2014 Definition &amp; IVF\/DMPK Uses\" \/>\n<meta property=\"og:description\" content=\"What is fatty acid free BSA, how is it made, and when is it required for IVF, PPAR assays, and DMPK microsomal protein binding?\" \/>\n<meta property=\"og:url\" content=\"https:\/\/seamlessbio.de\/de\/what-is-fatty-acid-free-bsa\/\" \/>\n<meta property=\"og:site_name\" content=\"SeamlessBio\" \/>\n<meta property=\"article:modified_time\" content=\"2026-11-02T15:25:02+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/seamlessbio.de\/wp-content\/uploads\/2026\/04\/134428046.png\" \/>\n\t<meta property=\"og:image:width\" content=\"973\" \/>\n\t<meta property=\"og:image:height\" content=\"288\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Gesch\u00e4tzte Lesezeit\" \/>\n\t<meta name=\"twitter:data1\" content=\"2\u00a0Minuten\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/seamlessbio.de\\\/what-is-fatty-acid-free-bsa\\\/\",\"url\":\"https:\\\/\\\/seamlessbio.de\\\/what-is-fatty-acid-free-bsa\\\/\",\"name\":\"What is Fatty Acid Free BSA? \u2014 Definition & IVF\\\/DMPK Uses\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/seamlessbio.de\\\/#website\"},\"datePublished\":\"2026-08-22T20:09:17+00:00\",\"dateModified\":\"2026-11-02T15:25:02+00:00\",\"description\":\"What is fatty acid free BSA, how is it made, and when is it required for IVF, PPAR assays, and DMPK microsomal protein binding?\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/seamlessbio.de\\\/what-is-fatty-acid-free-bsa\\\/#breadcrumb\"},\"inLanguage\":\"de\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/seamlessbio.de\\\/what-is-fatty-acid-free-bsa\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/seamlessbio.de\\\/what-is-fatty-acid-free-bsa\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Start\",\"item\":\"https:\\\/\\\/seamlessbio.de\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"What is Fatty Acid Free BSA?\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/seamlessbio.de\\\/#website\",\"url\":\"https:\\\/\\\/seamlessbio.de\\\/\",\"name\":\"SeamlessBio\",\"description\":\"Human serum, plasma and hPL, single-use bioprocessing bags, serum-free media, animal sera and FBS. 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