{"id":3093,"date":"2026-08-22T21:23:13","date_gmt":"2026-08-22T20:23:13","guid":{"rendered":"https:\/\/seamlessbio.de\/?page_id=3093"},"modified":"2026-08-22T23:53:49","modified_gmt":"2026-08-22T22:53:49","slug":"what-is-serum-free-medium","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/what-is-serum-free-medium\/","title":{"rendered":"What is Animal Component-Free (ACF) Cell Culture?"},"content":{"rendered":"
\n\t\t\t\t
\n \t\t
\n\t\t\t\t\t\n
\n

Quick Definition<\/p>\n

What is Animal Component-Free (ACF) Cell Culture?:<\/strong> Animal component-free (ACF) cell culture means no ingredients derived from any animal source \u2014 including human \u2014 are present in the medium. All components must be synthetic or produced by recombinant expression in microbial or plant systems. ACF is the highest standard for raw material origin control and is increasingly required for clinical-grade ATMP manufacturing.<\/p>\n<\/div>\n

The terminology hierarchy: serum-free \u2192 xeno-free \u2192 ACF<\/h2>\n

These represent progressively stricter raw material origin standards. Serum-free: no whole serum but BSA and bovine transferrin still allowed. Xeno-free: no foreign-species components \u2014 human-derived hPL and rHSA are allowed for human cells. ACF: no animal components of any origin including human \u2014 only synthetic and recombinant microbial\/plant-expressed components.<\/p>\n

ACF components from SeamlessBio<\/h2>\n

OsrhHSA (rice-expressed recombinant human serum albumin) and OsrhTF (rice-expressed recombinant human transferrin) form the ACF-compatible albumin and iron delivery base for human cell culture in GMP and ATMP applications.<\/p>\n

Key Facts<\/h2>\n