{"id":672,"date":"2026-05-14T16:20:40","date_gmt":"2026-05-14T15:20:40","guid":{"rendered":"http:\/\/seamlessbio.de\/?page_id=672"},"modified":"2026-06-27T17:24:54","modified_gmt":"2026-06-27T16:24:54","slug":"serum-free-media-hek293","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/serum-free-media-hek293\/","title":{"rendered":"Serumfreies Medium HEK293"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"672\" class=\"elementor elementor-672\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4b98307 e-con e-atomic-element e-flexbox-base e-92577b7 \" data-id=\"4b98307\" data-element_type=\"e-flexbox\" data-e-type=\"e-flexbox\" data-interaction-id=\"4b98307\">\n    \t\t<div class=\"elementor-element elementor-element-3fbcafb elementor-widget elementor-widget-html\" data-id=\"3fbcafb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t\t<!--\nYOAST SEO \u2014 seamlessbio.de\nSlug:        \/products\/cell-culture-media-reagents\/custom-serum-free-media-for-hek293-cho-mammalian-cell-lines\/serum-free-media-for-hek293-cells\/\nFocus KW:    serum-free media HEK293\nSEO Title:   Serum-Free Media for HEK293 Cells | AAV & Lentivirus Production | SeamlessBio\nMeta Desc:   Custom serum-free and chemically defined media for HEK293, HEK293T and HEK293F cells. Adherent and suspension formats for AAV production, lentiviral vectors and recombinant protein expression. EU supplier.\nBreadcrumb:  Home \u203a Products \u203a Cell Culture Media \u203a Custom Serum-Free Media \u203a HEK293\n-->\n<script type=\"application\/ld+json\">\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@graph\": [\n    {\n      \"@type\": \"BreadcrumbList\",\n      \"itemListElement\": [\n        { \"@type\": \"ListItem\", \"position\": 1, \"name\": \"Home\", \"item\": \"https:\/\/www.seamlessbio.de\/\" },\n        { \"@type\": \"ListItem\", \"position\": 2, \"name\": \"Products\", \"item\": \"https:\/\/www.seamlessbio.de\/products\/\" },\n        { \"@type\": \"ListItem\", \"position\": 3, \"name\": \"Cell Culture Media\", \"item\": \"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/\" },\n        { \"@type\": \"ListItem\", \"position\": 4, \"name\": \"Custom Serum-Free Media\", \"item\": \"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/custom-serum-free-media-for-hek293-cho-mammalian-cell-lines\/\" },\n        { \"@type\": \"ListItem\", \"position\": 5, \"name\": \"HEK293 Serum-Free Media\", \"item\": \"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/custom-serum-free-media-for-hek293-cho-mammalian-cell-lines\/serum-free-media-for-hek293-cells\/\" }\n      ]\n    },\n    {\n      \"@type\": \"FAQPage\",\n      \"mainEntity\": [\n        { \"@type\": \"Question\", \"name\": \"What serum-free media is best for HEK293 AAV production?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"For AAV production in HEK293 cells, a chemically defined, animal-origin-free suspension medium supporting high-density growth (6\u201310 \u00d7 10\u2076 cells\/mL) and compatibility with PEI-mediated triple transfection is recommended. SeamlessBio offers custom serum-free HEK293 media optimised for adherent and suspension AAV production workflows.\" } },\n        { \"@type\": \"Question\", \"name\": \"Can HEK293T cells be adapted to serum-free suspension culture?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"Yes. HEK293T cells can be adapted to serum-free suspension culture for scalable lentiviral vector and recombinant protein production. Adaptation typically requires 3\u20136 passages using a gradual serum reduction protocol, which SeamlessBio's technical team can guide you through.\" } },\n        { \"@type\": \"Question\", \"name\": \"What is the difference between HEK293, HEK293T and HEK293F?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"HEK293 is the parental cell line. HEK293T carries the SV40 Large T antigen for episomal plasmid amplification \u2014 widely used for lentiviral and AAV vector production. HEK293F is an adapted suspension variant from Thermo Fisher. HEK293-EBNA carries Epstein-Barr virus nuclear antigen. Each variant may require slightly different serum-free formulations.\" } },\n        { \"@type\": \"Question\", \"name\": \"What volumes are available for custom HEK293 serum-free media?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"SeamlessBio supplies custom HEK293 serum-free media from 500 mL research quantities to 1000+ litres for production scale. Liquid and powder formats are available. Contact info@seamlessbio.de for pricing and lead times.\" } }\n      ]\n    }\n  ]\n}\n<\/script>\n\n<section class=\"sb-hek-page\">\n<div class=\"sb-hek-container\">\n\n  <!-- BREADCRUMB -->\n  <nav class=\"sb-hek-bc\">\n    <a href=\"https:\/\/www.seamlessbio.de\/\">Home<\/a><span>\/<\/span>\n    <a href=\"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/\">Cell Culture Media<\/a><span>\/<\/span>\n    <a href=\"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/custom-serum-free-media-for-hek293-cho-mammalian-cell-lines\/\">Custom Serum-Free Media<\/a><span>\/<\/span>\n    <strong>HEK293 Cells<\/strong>\n  <\/nav>\n\n  <!-- HERO -->\n  <div class=\"sb-hek-hero\">\n    <div class=\"sb-hek-hero-text\">\n      <span class=\"sb-hek-eyebrow\">Custom Serum-Free Media \u00b7 EU Produced<\/span>\n      <h1>Serum-Free Media for HEK293 Cells<\/h1>\n      <p>Chemically defined, animal-origin-free cell culture media for HEK293, HEK293T, HEK293F and HEK293-EBNA \u2014 optimised for adherent and suspension culture, AAV production, lentiviral vector manufacturing and recombinant protein expression.<\/p>\n      <div class=\"sb-hek-hero-tags\">\n        <span>HEK293 \/ HEK293T \/ HEK293F<\/span>\n        <span>AAV Production<\/span>\n        <span>Lentiviral Vectors<\/span>\n        <span>Suspension & Adherent<\/span>\n        <span>GMP-Ready Pathway<\/span>\n      <\/div>\n      <div class=\"sb-hek-hero-btns\">\n        <a href=\"https:\/\/www.seamlessbio.de\/about-us\/contact-imprint\/\" class=\"sb-hek-btn\">Request Custom Formulation<\/a>\n        <a href=\"mailto:info@seamlessbio.de\" class=\"sb-hek-btn-outline\">info@seamlessbio.de<\/a>\n      <\/div>\n    <\/div>\n    <div class=\"sb-hek-hero-vis\">\n      <div class=\"sb-hek-cell-card\">\n        <div class=\"sb-hek-cell-icon\">\ud83e\uddeb<\/div>\n        <div class=\"sb-hek-cell-lines\">\n          <div class=\"sb-hek-cl\"><strong>HEK293<\/strong> \u2014 parental<\/div>\n          <div class=\"sb-hek-cl\"><strong>HEK293T<\/strong> \u2014 SV40 T-Ag, lentivirus\/AAV<\/div>\n          <div class=\"sb-hek-cl\"><strong>HEK293F<\/strong> \u2014 suspension adapted<\/div>\n          <div class=\"sb-hek-cl\"><strong>HEK293FT<\/strong> \u2014 high-titre lentivirus<\/div>\n          <div class=\"sb-hek-cl\"><strong>HEK293-EBNA<\/strong> \u2014 episomal expression<\/div>\n        <\/div>\n      <\/div>\n    <\/div>\n  <\/div>\n\n  <!-- FORMATS -->\n  <div class=\"sb-hek-section\">\n    <h2>Two Formats for Every Workflow<\/h2>\n    <div class=\"sb-hek-format-grid\">\n\n      <div class=\"sb-hek-format-card\">\n        <div class=\"sb-hek-format-badge\">Adherent<\/div>\n        <h3>Serum-Free Media for Adherent HEK293<\/h3>\n        <p>Chemically defined formulation for HEK293, HEK293T and HEK293FT in monolayer culture \u2014 T-flasks, multi-layer flasks and CellSTACK systems. Optimised for high transfection efficiency with PEI and lipid-based reagents.<\/p>\n        <table class=\"sb-hek-spec-table\">\n          <tr><td>Culture format<\/td><td>Monolayer \u2014 T-flask to multi-layer<\/td><\/tr>\n          <tr><td>Primary application<\/td><td>AAV, lentivirus, adenovirus production<\/td><\/tr>\n          <tr><td>Transfection<\/td><td>PEI, Lipofectamine, Ca-phosphate compatible<\/td><\/tr>\n          <tr><td>Key feature<\/td><td>Rapid cell attachment, consistent morphology<\/td><\/tr>\n          <tr><td>Animal-origin free<\/td><td>Yes<\/td><\/tr>\n        <\/table>\n      <\/div>\n\n      <div class=\"sb-hek-format-card\">\n        <div class=\"sb-hek-format-badge sb-hek-badge-sus\">Suspension<\/div>\n        <h3>Serum-Free Media for Suspension HEK293<\/h3>\n        <p>High-density suspension medium for HEK293F, HEK293-S and suspension-adapted HEK293T. Compatible with shake flask, wave bag and stirred-tank bioreactor formats for scalable vector and protein production.<\/p>\n        <table class=\"sb-hek-spec-table\">\n          <tr><td>Culture format<\/td><td>Shake flask \u00b7 Wave bag \u00b7 Bioreactor<\/td><\/tr>\n          <tr><td>Cell density<\/td><td>Up to 8\u201310 \u00d7 10\u2076 cells\/mL<\/td><\/tr>\n          <tr><td>Primary application<\/td><td>Scalable AAV, recombinant protein, VLP<\/td><\/tr>\n          <tr><td>Transfection<\/td><td>PEI-Max compatible<\/td><\/tr>\n          <tr><td>Animal-origin free<\/td><td>Yes<\/td><\/tr>\n        <\/table>\n      <\/div>\n\n    <\/div>\n  <\/div>\n\n  <!-- APPLICATIONS -->\n  <div class=\"sb-hek-section sb-hek-alt\">\n    <h2>Applications<\/h2>\n    <div class=\"sb-hek-app-grid\">\n      <div class=\"sb-hek-app-card\">\n        <h3>AAV Vector Production<\/h3>\n        <p>Serum-free HEK293 medium for adeno-associated virus manufacturing via triple transfection. Supports high-titer AAV2, AAV5, AAV8, AAV9 and novel serotypes for gene therapy programs.<\/p>\n      <\/div>\n      <div class=\"sb-hek-app-card\">\n        <h3>Lentiviral Vector Manufacturing<\/h3>\n        <p>Optimised for HEK293T triple plasmid transfection for lentiviral vector production. Supports 3rd and 4th generation packaging systems for CAR-T and gene correction applications.<\/p>\n      <\/div>\n      <div class=\"sb-hek-app-card\">\n        <h3>Adenoviral Vector Production<\/h3>\n        <p>Chemically defined medium for adenovirus propagation in HEK293 cells. Compatible with both adherent and suspension production formats for vaccine and gene therapy vectors.<\/td>\n      <\/div>\n      <div class=\"sb-hek-app-card\">\n        <h3>Recombinant Protein Expression<\/h3>\n        <p>High-yield transient and stable protein expression in HEK293 suspension culture. Protein-free formulation simplifies downstream purification of recombinant antibodies, enzymes and glycoproteins.<\/p>\n      <\/div>\n      <div class=\"sb-hek-app-card\">\n        <h3>VLP & Pseudovirus Production<\/h3>\n        <p>Supports virus-like particle (VLP) and pseudovirus production for vaccine development, neutralising antibody assays and antigen display platforms.<\/p>\n      <\/div>\n      <div class=\"sb-hek-app-card\">\n        <h3>Cell Line Development<\/h3>\n        <p>Serum-free medium for stable HEK293 clone selection and expansion. Reduces variability during CHO-like fed-batch development programmes in HEK293-based expression systems.<\/p>\n      <\/div>\n    <\/div>\n  <\/div>\n\n  <!-- COMPARISON -->\n  <div class=\"sb-hek-section\">\n    <h2>HEK293 Serum-Free vs Serum-Containing Culture<\/h2>\n    <div class=\"sb-hek-table-wrap\">\n      <table class=\"sb-hek-cmp-table\">\n        <thead>\n          <tr><th>Parameter<\/th><th>Serum-Free (SeamlessBio)<\/th><th>FBS-Supplemented<\/th><\/tr>\n        <\/thead>\n        <tbody>\n          <tr><td>Batch-to-batch consistency<\/td><td>\u2705 Excellent \u2014 defined composition<\/td><td>Variable \u2014 FBS lot-dependent<\/td><\/tr>\n          <tr><td>Downstream purification<\/td><td>\u2705 Simplified \u2014 no serum proteins<\/td><td>Complex \u2014 serum protein contamination<\/td><\/tr>\n          <tr><td>Regulatory compliance<\/td><td>\u2705 AOF \/ xeno-free documentation<\/td><td>\u26a0\ufe0f BSE\/TSE risk documentation required<\/td><\/tr>\n          <tr><td>Transfection efficiency<\/td><td>\u2705 Equivalent or superior<\/td><td>Reference<\/td><\/tr>\n          <tr><td>Scale-up to bioreactor<\/td><td>\u2705 Direct \u2014 suspension format available<\/td><td>Requires adaptation<\/td><\/tr>\n          <tr><td>GMP pathway<\/td><td>\u2705 In development<\/td><td>\u26a0\ufe0f Challenging \u2014 serum supply variability<\/td><\/tr>\n        <\/tbody>\n      <\/table>\n    <\/div>\n  <\/div>\n\n  <!-- PRODUCT INFO -->\n  <div class=\"sb-hek-section sb-hek-alt\">\n    <h2>Product Information<\/h2>\n    <table class=\"sb-hek-info-table\">\n      <tr><td>Available formats<\/td><td>Adherent (HEK293, HEK293T) \u00b7 Suspension (HEK293F, HEK293-S, suspension-adapted)<\/td><\/tr>\n      <tr><td>Classification<\/td><td>Serum-free \u00b7 Chemically defined \u00b7 Animal-origin-free (AOF)<\/td><\/tr>\n      <tr><td>Volumes<\/td><td>500 mL \u2013 1000 L \u00b7 Liquid and powder available<\/td><\/tr>\n      <tr><td>Transfection compatibility<\/td><td>PEI, PEI-Max, Lipofectamine, Ca-phosphate<\/td><\/tr>\n      <tr><td>Production<\/td><td>EU \u2014 ISO-controlled conditions<\/td><\/tr>\n      <tr><td>Quality documentation<\/td><td>CoA, CoO, endotoxin, sterility, osmolality, cell growth performance<\/td><\/tr>\n      <tr><td>GMP pathway<\/td><td>In development \u2014 contact us for timeline and requirements<\/td><\/tr>\n      <tr><td>Custom formulation<\/td><td>Available \u2014 DoE-based optimisation for your specific clone and process<\/td><\/tr>\n      <tr><td>Regulatory status<\/td><td>For research use only (GMP pathway in development)<\/td><\/tr>\n      <tr><td>Lead time<\/td><td>Standard: 5\u201310 days \u00b7 Custom development: 4\u201316 weeks<\/td><\/tr>\n    <\/table>\n  <\/div>\n\n  <!-- FAQ -->\n  <div class=\"sb-hek-section\">\n    <h2>Frequently Asked Questions<\/h2>\n    <div class=\"sb-hek-faqs\">\n      <div class=\"sb-hek-faq\">\n        <h3>What serum-free media is best for AAV production in HEK293?<\/h3>\n        <p>For scalable AAV production, a suspension-format, chemically defined medium supporting growth to 6\u201310 \u00d7 10\u2076 cells\/mL with PEI-Max transfection compatibility is recommended. Our formulation is optimised for triple plasmid transfection systems used in standard AAV production workflows.<\/p>\n      <\/div>\n      <div class=\"sb-hek-faq\">\n        <h3>Can HEK293T cells be adapted to serum-free suspension?<\/h3>\n        <p>Yes. HEK293T can be adapted to serum-free suspension culture using a stepwise serum reduction protocol over 3\u20136 passages. Our technical team provides adaptation protocols and technical support throughout the transition.<\/p>\n      <\/div>\n      <div class=\"sb-hek-faq\">\n        <h3>Is the medium compatible with PEI transfection?<\/h3>\n        <p>Yes \u2014 both the adherent and suspension formats are optimised for PEI (linear 25 kDa) and PEI-Max transfection, which is the standard reagent for HEK293-based vector production at research and pilot scale.<\/p>\n      <\/div>\n      <div class=\"sb-hek-faq\">\n        <h3>Can you develop a media formulation specific to my HEK293 clone?<\/h3>\n        <p>Yes. Our custom media development service uses DoE-based screening to optimise formulations for your specific cell line, transfection protocol and target product. Contact info@seamlessbio.de to discuss your requirements.<\/p>\n      <\/div>\n    <\/div>\n  <\/div>\n\n  <!-- RELATED -->\n  <div class=\"sb-hek-section\">\n    <h2>Related Products & Guides<\/h2>\n    <div class=\"sb-hek-related\">\n      <a href=\"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/custom-serum-free-media-for-hek293-cho-mammalian-cell-lines\/serum-free-media-for-cho-cells\/\" class=\"sb-hek-rel-card\">\n        <h3>Serum-Free Media for CHO Cells<\/h3>\n        <p>Chemically defined CHO-K1, CHO-S and CHO DG44 media for mAb and therapeutic protein manufacturing.<\/p>\n      <\/a>\n      <a href=\"https:\/\/www.seamlessbio.de\/applications\/aav-viral-vector-production\/\" class=\"sb-hek-rel-card\">\n        <h3>AAV & Viral Vector Production<\/h3>\n        <p>Application guide: media, serum and process considerations for HEK293-based AAV manufacturing.<\/p>\n      <\/a>\n      <a href=\"https:\/\/www.seamlessbio.de\/applications\/cell-therapy-atmps\/\" class=\"sb-hek-rel-card\">\n        <h3>Cell Therapy & ATMP Manufacturing<\/h3>\n        <p>Media components for ATMP pipelines \u2014 from vector production to final cell therapy product.<\/p>\n      <\/a>\n      <a href=\"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/custom-serum-free-media-for-hek293-cho-mammalian-cell-lines\/\" class=\"sb-hek-rel-card\">\n        <h3>All Serum-Free Media<\/h3>\n        <p>VERO, hybridoma, T-cell, MSC and custom formulations for any mammalian cell line.<\/p>\n      <\/a>\n    <\/div>\n  <\/div>\n\n  <!-- CTA -->\n  <div class=\"sb-hek-cta\">\n    <h2>Request Your HEK293 Serum-Free Medium<\/h2>\n    <p>Standard formulations or custom development for your clone and process. EU documentation included.<\/p>\n    <div class=\"sb-hek-cta-btns\">\n      <a href=\"https:\/\/www.seamlessbio.de\/about-us\/contact-imprint\/\" class=\"sb-hek-btn\">Contact Us<\/a>\n      <a href=\"mailto:info@seamlessbio.de\" class=\"sb-hek-btn-outline-w\">info@seamlessbio.de<\/a>\n    <\/div>\n    <p class=\"sb-hek-ruo\">For research use only.<\/p>\n  <\/div>\n\n<\/div>\n<\/section>\n\n<style>\n.sb-hek-page, .sb-hek-page *, .sb-hek-page *::before, .sb-hek-page *::after { box-sizing: border-box; }\n.sb-hek-page { font-family: Arial, sans-serif; color: #1a1a1a; background: #fff; padding: 60px 32px 90px; }\n.sb-hek-container { max-width: 1200px; margin: 0 auto; }\n.sb-hek-bc { display: flex; flex-wrap: wrap; gap: 6px; align-items: center; font-size: 13px; color: #888; margin-bottom: 28px; }\n.sb-hek-bc a { color: #18b7b2 !important; text-decoration: none !important; font-weight: 600; }\n.sb-hek-bc strong { color: #111; }\n.sb-hek-bc span { color: #ccc; }\n.sb-hek-hero { display: grid; 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