{"id":673,"date":"2026-05-14T16:20:40","date_gmt":"2026-05-14T15:20:40","guid":{"rendered":"http:\/\/seamlessbio.de\/?page_id=673"},"modified":"2026-06-27T17:44:58","modified_gmt":"2026-06-27T16:44:58","slug":"serum-free-media-cho","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/serum-free-media-cho\/","title":{"rendered":"Serumfreies CHO-Medium"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"673\" class=\"elementor elementor-673\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f1ddb5a e-con e-atomic-element e-flexbox-base e-1f1e3bc \" data-id=\"f1ddb5a\" data-element_type=\"e-flexbox\" data-e-type=\"e-flexbox\" data-interaction-id=\"f1ddb5a\">\n    \t\t<div class=\"elementor-element elementor-element-d41e587 elementor-widget elementor-widget-html\" data-id=\"d41e587\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t\t<!--\nYOAST SEO \u2014 seamlessbio.de\nSlug:        \/products\/cell-culture-media-reagents\/custom-serum-free-media-for-hek293-cho-mammalian-cell-lines\/serum-free-media-for-cho-cells\/\nFocus KW:    serum-free media CHO cells\nSEO Title:   Serum-Free Media for CHO Cells | mAb & Protein Production | SeamlessBio\nMeta Desc:   Chemically defined, protein-free serum-free media for CHO-K1, CHO-S and CHO DG44. Optimised for monoclonal antibody production, fed-batch and perfusion processes. EU supplier. Custom formulation available.\nBreadcrumb:  Home \u203a Products \u203a Cell Culture Media \u203a Custom Serum-Free Media \u203a CHO Cells\n-->\n<script type=\"application\/ld+json\">\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@graph\": [\n    {\n      \"@type\": \"BreadcrumbList\",\n      \"itemListElement\": [\n        { \"@type\": \"ListItem\", \"position\": 1, \"name\": \"Home\", \"item\": \"https:\/\/www.seamlessbio.de\/\" },\n        { \"@type\": \"ListItem\", \"position\": 2, \"name\": \"Products\", \"item\": \"https:\/\/www.seamlessbio.de\/products\/\" },\n        { \"@type\": \"ListItem\", \"position\": 3, \"name\": \"Cell Culture Media\", \"item\": \"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/\" },\n        { \"@type\": \"ListItem\", \"position\": 4, \"name\": \"Custom Serum-Free Media\", \"item\": \"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/custom-serum-free-media-for-hek293-cho-mammalian-cell-lines\/\" },\n        { \"@type\": \"ListItem\", \"position\": 5, \"name\": \"CHO Serum-Free Media\", \"item\": \"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/custom-serum-free-media-for-hek293-cho-mammalian-cell-lines\/serum-free-media-for-cho-cells\/\" }\n      ]\n    },\n    {\n      \"@type\": \"FAQPage\",\n      \"mainEntity\": [\n        { \"@type\": \"Question\", \"name\": \"What is chemically defined CHO medium?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"Chemically defined CHO medium is a serum-free, protein-free cell culture formulation where all components are identified at known concentrations. This provides maximum batch-to-batch consistency, simplified downstream purification, and the regulatory documentation required for GMP biopharmaceutical manufacturing.\" } },\n        { \"@type\": \"Question\", \"name\": \"Which CHO cell lines are compatible with SeamlessBio serum-free media?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"Our formulations are compatible with CHO-K1, CHO-S, CHO DG44, CHO-DHFR\u207b and other major CHO host cell lines. Custom optimisation is available for specific clones.\" } },\n        { \"@type\": \"Question\", \"name\": \"Are feed supplements available for fed-batch CHO culture?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"Yes. SeamlessBio offers matched feed supplements for fed-batch and perfusion CHO culture. Feed supplements are available in concentrated liquid and dry powder formats, customisable to your feeding strategy and feeding schedule.\" } },\n        { \"@type\": \"Question\", \"name\": \"Can CHO serum-free media be used in perfusion culture?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"Yes. Our chemically defined CHO formulations are compatible with perfusion culture systems including hollow-fibre bioreactors, ATF and TFF perfusion. Contact us to discuss perfusion-specific formulation requirements.\" } }\n      ]\n    }\n  ]\n}\n<\/script>\n\n<section class=\"sb-cho-page\">\n<div class=\"sb-cho-container\">\n\n  <!-- BREADCRUMB -->\n  <nav class=\"sb-cho-bc\">\n    <a href=\"https:\/\/www.seamlessbio.de\/\">Home<\/a><span>\/<\/span>\n    <a href=\"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/\">Cell Culture Media<\/a><span>\/<\/span>\n    <a href=\"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/custom-serum-free-media-for-hek293-cho-mammalian-cell-lines\/\">Custom Serum-Free Media<\/a><span>\/<\/span>\n    <strong>CHO Cells<\/strong>\n  <\/nav>\n\n  <!-- HERO -->\n  <div class=\"sb-cho-hero\">\n    <div class=\"sb-cho-hero-text\">\n      <span class=\"sb-cho-eyebrow\">Chemically Defined \u00b7 Protein-Free \u00b7 EU Produced<\/span>\n      <h1>Serum-Free Media for CHO Cells<\/h1>\n      <p>Chemically defined, protein-free, animal-origin-free media for Chinese hamster ovary cells \u2014 optimised for monoclonal antibody production, therapeutic protein expression, and biosimilar manufacturing in fed-batch and perfusion formats.<\/p>\n      <div class=\"sb-cho-hero-tags\">\n        <span>CHO-K1 \u00b7 CHO-S \u00b7 CHO DG44<\/span>\n        <span>mAb Production<\/span>\n        <span>Fed-Batch & Perfusion<\/span>\n        <span>Protein-Free<\/span>\n        <span>GMP-Ready Pathway<\/span>\n      <\/div>\n      <div class=\"sb-cho-hero-btns\">\n        <a href=\"https:\/\/www.seamlessbio.de\/about-us\/contact-imprint\/\" class=\"sb-cho-btn\">Request Formulation<\/a>\n        <a href=\"mailto:info@seamlessbio.de\" class=\"sb-cho-btn-outline\">info@seamlessbio.de<\/a>\n      <\/div>\n    <\/div>\n    <div class=\"sb-cho-hero-vis\">\n      <div class=\"sb-cho-stats-vis\">\n        <div class=\"sb-cho-sv\">\n          <span class=\"sb-cho-sv-n\">~70%<\/span>\n          <span>of all approved recombinant therapeutics are produced in CHO cells<\/span>\n        <\/div>\n        <div class=\"sb-cho-sv\">\n          <span class=\"sb-cho-sv-n\">CD<\/span>\n          <span>Chemically defined \u2014 all components identified at known concentrations<\/span>\n        <\/div>\n        <div class=\"sb-cho-sv\">\n          <span class=\"sb-cho-sv-n\">AOF<\/span>\n          <span>Animal-origin-free \u2014 no serum, no undefined animal components<\/span>\n        <\/div>\n      <\/div>\n    <\/div>\n  <\/div>\n\n  <!-- CHO CELL LINES -->\n  <div class=\"sb-cho-section\">\n    <h2>Compatible CHO Cell Lines<\/h2>\n    <div class=\"sb-cho-cl-grid\">\n      <div class=\"sb-cho-cl-card\">\n        <div class=\"sb-cho-cl-tag\">CHO-K1<\/div>\n        <p>Most widely used CHO host cell line. Suitable for transient expression and stable cell line development for therapeutic proteins and biosimilars.<\/p>\n      <\/div>\n      <div class=\"sb-cho-cl-card\">\n        <div class=\"sb-cho-cl-tag\">CHO-S<\/div>\n        <p>Suspension-adapted CHO variant for scalable bioreactor production. High growth rate and tolerance for serum-free suspension culture.<\/p>\n      <\/div>\n      <div class=\"sb-cho-cl-card\">\n        <div class=\"sb-cho-cl-tag\">CHO DG44<\/div>\n        <p>DHFR-deficient CHO line for gene amplification. Standard platform for high-expression stable cell line development with MTX selection.<\/p>\n      <\/div>\n      <div class=\"sb-cho-cl-card\">\n        <div class=\"sb-cho-cl-tag\">CHO-DHFR\u207b<\/div>\n        <p>Alternative DHFR-negative line for gene amplification. Compatible with both DHFR and glutamine synthetase (GS) selection systems.<\/p>\n      <\/div>\n    <\/div>\n  <\/div>\n\n  <!-- PROCESS FORMATS -->\n  <div class=\"sb-cho-section sb-cho-alt\">\n    <h2>Optimised for Your Process Format<\/h2>\n    <div class=\"sb-cho-proc-grid\">\n      <div class=\"sb-cho-proc-card\">\n        <h3>Batch Culture<\/h3>\n        <p>Basal medium for standard batch culture in shake flask and bioreactor. Consistent performance for process development and early-stage production.<\/p>\n        <div class=\"sb-cho-proc-spec\">Shake flask \u00b7 Bioreactor seed train \u00b7 Process development<\/div>\n      <\/div>\n      <div class=\"sb-cho-proc-card\">\n        <h3>Fed-Batch Culture<\/h3>\n        <p>Basal medium paired with matched concentrated feed supplements. Extended culture duration with maintained viability and product quality. Compatible with bolus and continuous feeding strategies.<\/p>\n        <div class=\"sb-cho-proc-spec\">Wave bag \u00b7 Stirred-tank bioreactor \u00b7 7\u201321 day runs<\/div>\n      <\/div>\n      <div class=\"sb-cho-proc-card\">\n        <h3>Perfusion Culture<\/h3>\n        <p>Chemically defined formulation supporting high-density perfusion processes with ATF, TFF and hollow-fibre systems. Maintains cell viability above 90% at high specific perfusion rates.<\/p>\n        <div class=\"sb-cho-proc-spec\">ATF \u00b7 TFF \u00b7 Hollow-fibre bioreactor \u00b7 Continuous manufacturing<\/div>\n      <\/div>\n    <\/div>\n  <\/div>\n\n  <!-- APPLICATIONS -->\n  <div class=\"sb-cho-section\">\n    <h2>Applications<\/h2>\n    <div class=\"sb-cho-app-grid\">\n      <div class=\"sb-cho-app-card\">\n        <h3>Monoclonal Antibody Production<\/h3>\n        <p>Chemically defined CHO medium for IgG1, IgG4 and bispecific antibody manufacturing. High-titre expression with consistent glycosylation profiles across batches.<\/p>\n      <\/div>\n      <div class=\"sb-cho-app-card\">\n        <h3>Therapeutic Protein Expression<\/h3>\n        <p>Recombinant enzymes, fusion proteins, cytokines and growth factors. Protein-free formulation simplifies downstream purification with reduced host cell protein contamination.<\/p>\n      <\/div>\n      <div class=\"sb-cho-app-card\">\n        <h3>Biosimilar Development<\/h3>\n        <p>Defined, consistent medium for biosimilar programmes requiring tight control of critical quality attributes (CQA) including glycosylation, charge variants and aggregation.<\/p>\n      <\/div>\n      <div class=\"sb-cho-app-card\">\n        <h3>Stable Cell Line Development<\/h3>\n        <p>Chemically defined medium for CHO clone selection, single-cell cloning and early-stage expansion. Compatible with DHFR, GS and CRISPR-based selection systems.<\/p>\n      <\/div>\n      <div class=\"sb-cho-app-card\">\n        <h3>Fc-Fusion Proteins<\/h3>\n        <p>Optimised for Fc-fusion protein expression in CHO with maintained Fc receptor binding activity. Used for TNFR-Fc, EPO-Fc and receptor-ligand fusion platforms.<\/p>\n      <\/div>\n      <div class=\"sb-cho-app-card\">\n        <h3>Process Development & DoE<\/h3>\n        <p>Consistent, defined medium as the basis for systematic media optimisation studies. Compatible with high-throughput ambr15\/ambr250 micro-bioreactor platforms.<\/p>\n      <\/div>\n    <\/div>\n  <\/div>\n\n  <!-- WHY SERUM-FREE -->\n  <div class=\"sb-cho-section sb-cho-alt\">\n    <h2>Why Chemically Defined CHO Medium?<\/h2>\n    <div class=\"sb-cho-table-wrap\">\n      <table class=\"sb-cho-cmp-table\">\n        <thead>\n          <tr><th>Parameter<\/th><th>Chemically Defined (SeamlessBio)<\/th><th>FBS-Supplemented<\/th><\/tr>\n        <\/thead>\n        <tbody>\n          <tr><td>Batch consistency<\/td><td>\u2705 Excellent \u2014 defined composition<\/td><td>Variable \u2014 FBS lot-dependent<\/td><\/tr>\n          <tr><td>Host cell protein (HCP)<\/td><td>\u2705 Lower \u2014 no serum proteins<\/td><td>High \u2014 serum protein background<\/td><\/tr>\n          <tr><td>Downstream purification<\/td><td>\u2705 Simplified, higher yield<\/td><td>Complex \u2014 serum protein removal required<\/td><\/tr>\n          <tr><td>Glycosylation control<\/td><td>\u2705 Consistent \u2014 defined nutrients<\/td><td>Variable \u2014 serum components affect glycosylation<\/td><\/tr>\n          <tr><td>Regulatory filing (BLA\/MAA)<\/td><td>\u2705 Clean documentation path<\/td><td>\u26a0\ufe0f BSE\/TSE risk \u2014 additional documentation<\/td><\/tr>\n          <tr><td>GMP compatibility<\/td><td>\u2705 Preferred for clinical manufacturing<\/td><td>\u26a0\ufe0f Acceptable but not preferred<\/td><\/tr>\n          <tr><td>Scale-up<\/td><td>\u2705 Consistent performance across scales<\/td><td>Scale-dependent FBS effects<\/td><\/tr>\n        <\/tbody>\n      <\/table>\n    <\/div>\n  <\/div>\n\n  <!-- PRODUCT INFO -->\n  <div class=\"sb-cho-section\">\n    <h2>Product Information<\/h2>\n    <table class=\"sb-cho-info-table\">\n      <tr><td>Compatible cell lines<\/td><td>CHO-K1, CHO-S, CHO DG44, CHO-DHFR\u207b and derivatives<\/td><\/tr>\n      <tr><td>Classification<\/td><td>Serum-free \u00b7 Chemically defined \u00b7 Protein-free \u00b7 Animal-origin-free<\/td><\/tr>\n      <tr><td>Process formats<\/td><td>Batch \u00b7 Fed-batch \u00b7 Perfusion<\/td><\/tr>\n      <tr><td>Feed supplements<\/td><td>Available \u2014 concentrated liquid and dry powder, matched formulations<\/td><\/tr>\n      <tr><td>Volumes<\/td><td>500 mL \u2013 1000 L \u00b7 Liquid and powder formats<\/td><\/tr>\n      <tr><td>Production<\/td><td>EU \u2014 ISO-controlled conditions<\/td><\/tr>\n      <tr><td>Quality documentation<\/td><td>CoA, CoO, endotoxin, sterility, osmolality, pH, cell growth performance<\/td><\/tr>\n      <tr><td>GMP pathway<\/td><td>In development \u2014 contact us for timeline and documentation requirements<\/td><\/tr>\n      <tr><td>Custom formulation<\/td><td>Available \u2014 DoE-based clone-specific optimisation<\/td><\/tr>\n      <tr><td>Regulatory status<\/td><td>For research use only (GMP pathway in development)<\/td><\/tr>\n      <tr><td>Lead time<\/td><td>Standard: 5\u201310 days \u00b7 Custom: 4\u201316 weeks<\/td><\/tr>\n    <\/table>\n  <\/div>\n\n  <!-- FAQ -->\n  <div class=\"sb-cho-section sb-cho-alt\">\n    <h2>Frequently Asked Questions<\/h2>\n    <div class=\"sb-cho-faqs\">\n      <div class=\"sb-cho-faq\">\n        <h3>What is chemically defined CHO medium and why does it matter?<\/h3>\n        <p>Chemically defined (CD) medium contains all components at known, fixed concentrations \u2014 no undefined serum proteins. This gives maximum batch-to-batch consistency, cleaner downstream processing, and the regulatory documentation required for GMP manufacturing and BLA\/MAA submissions.<\/p>\n      <\/div>\n      <div class=\"sb-cho-faq\">\n        <h3>Are feed supplements available for fed-batch culture?<\/h3>\n        <p>Yes \u2014 matched concentrated feed supplements are available in liquid (10\u201320\u00d7) and dry powder formats. Feed formulations are compatible with bolus, linear and exponential feeding strategies. Custom feed development is also available for clone-specific optimisation.<\/p>\n      <\/div>\n      <div class=\"sb-cho-faq\">\n        <h3>Can the medium be used in perfusion systems?<\/h3>\n        <p>Yes. Our CHO CD formulations are compatible with ATF, TFF and hollow-fibre perfusion systems. Perfusion-specific formulations (higher nutrient density, reduced osmolality drift) are available on request.<\/p>\n      <\/div>\n      <div class=\"sb-cho-faq\">\n        <h3>Can you develop a medium specific to my CHO clone?<\/h3>\n        <p>Yes. Our custom media development service covers DoE-based nutrient screening, amino acid optimisation, lipid and trace element adjustment, and feed strategy development \u2014 all targeting your specific clone's productivity and CQA requirements.<\/p>\n      <\/div>\n    <\/div>\n  <\/div>\n\n  <!-- RELATED -->\n  <div class=\"sb-cho-section\">\n    <h2>Related Products & Guides<\/h2>\n    <div class=\"sb-cho-related\">\n      <a href=\"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/custom-serum-free-media-for-hek293-cho-mammalian-cell-lines\/serum-free-media-for-hek293-cells\/\" class=\"sb-cho-rel-card\">\n        <h3>Serum-Free Media for HEK293<\/h3>\n        <p>Defined media for HEK293T and HEK293F \u2014 AAV, lentivirus and recombinant protein production.<\/p>\n      <\/a>\n      <a href=\"https:\/\/www.seamlessbio.de\/applications\/hybridoma-monoclonal-antibody-production\/\" class=\"sb-cho-rel-card\">\n        <h3>Hybridoma & mAb Production<\/h3>\n        <p>Application guide: media considerations for monoclonal antibody production in CHO and hybridoma systems.<\/p>\n      <\/a>\n      <a href=\"https:\/\/www.seamlessbio.de\/applications\/cell-therapy-atmps\/\" class=\"sb-cho-rel-card\">\n        <h3>Cell Therapy & ATMP Manufacturing<\/h3>\n        <p>Defined media for ATMP development \u2014 from CHO-produced biologics to cell-based therapies.<\/p>\n      <\/a>\n      <a href=\"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/custom-serum-free-media-for-hek293-cho-mammalian-cell-lines\/\" class=\"sb-cho-rel-card\">\n        <h3>All Serum-Free Media<\/h3>\n        <p>VERO, T-cell, MSC, hybridoma and custom formulations for any mammalian cell line.<\/p>\n      <\/a>\n    <\/div>\n  <\/div>\n\n  <!-- CTA -->\n  <div class=\"sb-cho-cta\">\n    <h2>Request Your CHO Serum-Free Medium<\/h2>\n    <p>Standard chemically defined formulations or custom clone-specific development. Feed supplements available. EU documentation included.<\/p>\n    <div class=\"sb-cho-cta-btns\">\n      <a href=\"https:\/\/www.seamlessbio.de\/about-us\/contact-imprint\/\" class=\"sb-cho-btn\">Contact Us<\/a>\n      <a href=\"mailto:info@seamlessbio.de\" class=\"sb-cho-btn-outline-w\">info@seamlessbio.de<\/a>\n    <\/div>\n    <p class=\"sb-cho-ruo\">For research use only.<\/p>\n  <\/div>\n\n<\/div>\n<\/section>\n\n<style>\n.sb-cho-page, .sb-cho-page *, .sb-cho-page *::before, .sb-cho-page *::after { box-sizing: border-box; }\n.sb-cho-page { font-family: Arial, sans-serif; color: #1a1a1a; background: #fff; padding: 60px 32px 90px; }\n.sb-cho-container { max-width: 1200px; margin: 0 auto; }\n.sb-cho-bc { display: flex; flex-wrap: wrap; gap: 6px; align-items: center; font-size: 13px; color: #888; margin-bottom: 28px; }\n.sb-cho-bc a { color: #18b7b2 !important; text-decoration: none !important; font-weight: 600; }\n.sb-cho-bc strong { color: #111; }\n.sb-cho-bc span { color: #ccc; }\n.sb-cho-hero { display: grid; 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