{"id":686,"date":"2026-05-14T16:20:41","date_gmt":"2026-05-14T15:20:41","guid":{"rendered":"http:\/\/seamlessbio.de\/?page_id=686"},"modified":"2026-06-21T20:20:13","modified_gmt":"2026-06-21T19:20:13","slug":"human-mrp2-vesicle-kit","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/human-mrp2-vesicle-kit\/","title":{"rendered":"MRP2-Vesikel-Kit"},"content":{"rendered":"
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\n Startseite<\/a>\n \/<\/span>\n DMPK Transporter Kit<\/a>\n \/<\/span>\n MRP2-Vesikel-Kit<\/strong>\n <\/div>\n\n
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\n SeamlessBio \u2014 Cell4Pharma DMPK Assays<\/span>\n

Human MRP2 Vesicle Kit<\/h1>\n

\n HEK293-derived inside-out membrane vesicles for MRP2 inhibition screening and drug-drug interaction (DDI) risk assessment, in line with FDA and EMA testing recommendations.\n <\/p>\n

\n Informationen anfordern<\/a>\n Im Shop ansehen<\/a>\n <\/div>\n <\/div>\n
\n \"Human\n
Platzhalter f\u00fcr Produktbild<\/div>\n <\/div>\n <\/div>\n\n
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\n 01<\/span>\n

BSEP-Related DILI Risk<\/h3>\n

\n Assessed alongside BSEP for compensatory bile acid efflux capacity and combined cholestasis risk.\n <\/p>\n <\/div>\n\n

\n 02<\/span>\n

Inside-Out Vesicles<\/h3>\n

\n HEK293-derived membrane vesicles with confirmed MRP2 expression and inside-out orientation for reliable, reproducible transport data.\n <\/p>\n <\/div>\n\n

\n 03<\/span>\n

100 Reactions per Kit<\/h3>\n

\n Each kit is sized for 100 reactions based on a 96-well plate\n format, suitable for IC50 determination and single-concentration\n screening.\n <\/p>\n <\/div>\n\n

\n 04<\/span>\n

Dry Ice Shipment<\/h3>\n

\n Shipped frozen at \u221280 \u00b0C on dry ice to preserve vesicle\n integrity and transport activity upon arrival.\n <\/p>\n <\/div>\n\n <\/div>\n\n

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Why MRP2 testing matters in DMPK programs<\/h2>\n

\n MRP2 (ABCC2) is a hepatic canalicular transporter that, alongside BSEP, mediates biliary excretion of conjugated metabolites and bile constituents.\n <\/p>\n

\n The vesicle-based assay uses estradiol-17\u03b2-glucuronide or DNP-SG as a probe substrate to quantify MRP2-mediated transport into inside-out membrane vesicles, in the presence and absence of test compounds, allowing determination of inhibition potency and IC50 values.\n <\/p>\n

\n MRP2 inhibition is mechanistically linked to impaired biliary excretion and is assessed alongside BSEP, MRP3 and MRP4 to evaluate compensatory bile acid efflux capacity in DILI risk assessment. Because MRP2 and BSEP share the hepatic canalicular membrane, combined inhibition of both transporters is considered a higher-risk DILI signal than inhibition of either alone.\n <\/p>\n <\/div>\n\n

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Typical applications<\/h3>\n