{"id":714,"date":"2026-05-14T16:20:42","date_gmt":"2026-05-14T15:20:42","guid":{"rendered":"http:\/\/seamlessbio.de\/?page_id=714"},"modified":"2026-06-27T17:48:53","modified_gmt":"2026-06-27T16:48:53","slug":"custom-cell-culture-media","status":"publish","type":"page","link":"https:\/\/seamlessbio.de\/de\/custom-cell-culture-media\/","title":{"rendered":"Ma\u00dfgeschneiderte Zellkulturmedien"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"714\" class=\"elementor elementor-714\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-615d203 e-con e-atomic-element e-flexbox-base e-fc50703 \" data-id=\"615d203\" data-element_type=\"e-flexbox\" data-e-type=\"e-flexbox\" data-interaction-id=\"615d203\">\n    \t\t<div class=\"elementor-element elementor-element-ac8d3e1 elementor-widget elementor-widget-html\" data-id=\"ac8d3e1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t\t<!--\nYOAST SEO \u2014 seamlessbio.de\nSlug:        \/products\/cell-culture-media-reagents\/custom-cell-culture-media\/\nFocus KW:    custom cell culture media\nSEO Title:   Custom Cell Culture Media | CDMO Formulation Service | SeamlessBio\nMeta Desc:   Custom cell culture media development and manufacturing \u2014 serum-free, chemically defined, xeno-free and animal-origin-free. CDMO service from 500 mL to 1000+ L. EU produced. SeamlessBio.\nBreadcrumb:  Home \u203a Products \u203a Cell Culture Media \u203a Custom Cell Culture Media\n-->\n<script type=\"application\/ld+json\">\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@graph\": [\n    {\n      \"@type\": \"BreadcrumbList\",\n      \"itemListElement\": [\n        { \"@type\": \"ListItem\", \"position\": 1, \"name\": \"Home\", \"item\": \"https:\/\/www.seamlessbio.de\/\" },\n        { \"@type\": \"ListItem\", \"position\": 2, \"name\": \"Products\", \"item\": \"https:\/\/www.seamlessbio.de\/products\/\" },\n        { \"@type\": \"ListItem\", \"position\": 3, \"name\": \"Cell Culture Media\", \"item\": \"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/\" },\n        { \"@type\": \"ListItem\", \"position\": 4, \"name\": \"Custom Cell Culture Media\", \"item\": \"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/custom-cell-culture-media\/\" }\n      ]\n    },\n    {\n      \"@type\": \"Service\",\n      \"name\": \"Custom Cell Culture Media Development & Manufacturing\",\n      \"provider\": { \"@type\": \"Organization\", \"name\": \"SeamlessBio GmbH\", \"url\": \"https:\/\/www.seamlessbio.de\" },\n      \"description\": \"Custom cell culture media formulation, development and manufacturing service. Serum-free, chemically defined, xeno-free and animal-origin-free media for mammalian cell lines. From 500 mL to 1000+ L. EU produced.\",\n      \"areaServed\": \"EU\",\n      \"serviceType\": \"CDMO \u2014 Custom Media Development & Manufacturing\"\n    },\n    {\n      \"@type\": \"FAQPage\",\n      \"mainEntity\": [\n        { \"@type\": \"Question\", \"name\": \"How long does custom cell culture media development take?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"Simple modifications to existing formulations take 2\u20134 weeks. Full custom media development from scratch takes 8\u201316 weeks. GMP-grade production with complete regulatory documentation takes 3\u20136 months. A detailed project plan is provided at project start.\" } },\n        { \"@type\": \"Question\", \"name\": \"What volumes can SeamlessBio supply for custom media?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"SeamlessBio supplies custom cell culture media from 500 mL for research use to 1000+ litres for production scale. Both liquid ready-to-use and dry powder formats are available. Multi-year supply agreements are available for commercial customers.\" } },\n        { \"@type\": \"Question\", \"name\": \"Can SeamlessBio develop GMP-grade custom media?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"Yes. SeamlessBio provides custom media development from research grade through GMP-compliant manufacturing under ISO 9001 and ISO 13485 conditions, with full regulatory documentation including CoA, CoO, stability data, and DMF support.\" } }\n      ]\n    }\n  ]\n}\n<\/script>\n\n<section class=\"sb-ccm2-page\">\n<div class=\"sb-ccm2-container\">\n\n  <!-- BREADCRUMB -->\n  <nav class=\"sb-ccm2-bc\">\n    <a href=\"https:\/\/www.seamlessbio.de\/\">Home<\/a><span>\/<\/span>\n    <a href=\"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/\">Cell Culture Media<\/a><span>\/<\/span>\n    <strong>Custom Cell Culture Media<\/strong>\n  <\/nav>\n\n  <!-- HERO -->\n  <div class=\"sb-ccm2-hero\">\n    <div class=\"sb-ccm2-hero-text\">\n      <span class=\"sb-ccm2-eyebrow\">CDMO Service \u00b7 EU Produced<\/span>\n      <h1>Custom Cell Culture Media<\/h1>\n      <p>Tailored cell culture media formulated for your cell line, process and regulatory requirements \u2014 from small research batches to commercial production volumes. Serum-free, chemically defined, xeno-free and animal-origin-free formulations available.<\/p>\n      <div class=\"sb-ccm2-hero-tags\">\n        <span>Any Cell Line<\/span>\n        <span>Serum-Free & Chemically Defined<\/span>\n        <span>500 mL to 1000+ L<\/span>\n        <span>DoE-Based Development<\/span>\n        <span>GMP-Ready Pathway<\/span>\n      <\/div>\n      <a href=\"https:\/\/www.seamlessbio.de\/about-us\/contact-imprint\/\" class=\"sb-ccm2-btn\">Start Your Project<\/a>\n    <\/div>\n    <div class=\"sb-ccm2-hero-vis\">\n      <div class=\"sb-ccm2-steps-vis\">\n        <div class=\"sb-ccm2-sv\"><span class=\"sb-ccm2-sv-n\">01<\/span><span>Share your formulation requirements<\/span><\/div>\n        <div class=\"sb-ccm2-sv-arrow\">\u2193<\/div>\n        <div class=\"sb-ccm2-sv\"><span class=\"sb-ccm2-sv-n\">02<\/span><span>DoE-based screening & optimisation<\/span><\/div>\n        <div class=\"sb-ccm2-sv-arrow\">\u2193<\/div>\n        <div class=\"sb-ccm2-sv\"><span class=\"sb-ccm2-sv-n\">03<\/span><span>Prototype batch with full QC<\/span><\/div>\n        <div class=\"sb-ccm2-sv-arrow\">\u2193<\/div>\n        <div class=\"sb-ccm2-sv\"><span class=\"sb-ccm2-sv-n\">04<\/span><span>Scale-up & commercial supply<\/span><\/div>\n      <\/div>\n    <\/div>\n  <\/div>\n\n  <!-- WHAT WE DEVELOP -->\n  <div class=\"sb-ccm2-section\">\n    <h2>What We Develop<\/h2>\n    <div class=\"sb-ccm2-type-grid\">\n      <div class=\"sb-ccm2-type-card\">\n        <div class=\"sb-ccm2-type-icon\">\ud83e\uddea<\/div>\n        <h3>Serum-Free Media<\/h3>\n        <p>Replace FBS with a defined, reproducible formulation. Retain performance while gaining regulatory compliance, simplified downstream processing and supply chain security.<\/p>\n        <div class=\"sb-ccm2-type-tags\"><span>Any mammalian cell line<\/span><span>Research to GMP<\/span><\/div>\n      <\/div>\n      <div class=\"sb-ccm2-type-card\">\n        <div class=\"sb-ccm2-type-icon\">\u2697\ufe0f<\/div>\n        <h3>Chemically Defined Media<\/h3>\n        <p>All components identified at known concentrations. Maximum batch-to-batch consistency and full regulatory transparency for BLA, MAA and IMPD submissions.<\/p>\n        <div class=\"sb-ccm2-type-tags\"><span>CHO, HEK293, VERO, hybridoma<\/span><span>GMP preferred<\/span><\/div>\n      <\/div>\n      <div class=\"sb-ccm2-type-card\">\n        <div class=\"sb-ccm2-type-icon\">\ud83c\udf3f<\/div>\n        <h3>Xeno-Free Media<\/h3>\n        <p>Human-derived or recombinant components only \u2014 no non-human animal materials. Required for cell therapy products intended for clinical use and ATMP manufacturing.<\/p>\n        <div class=\"sb-ccm2-type-tags\"><span>T-cells, MSCs, NK cells<\/span><span>CAR-T, regenerative medicine<\/span><\/div>\n      <\/div>\n      <div class=\"sb-ccm2-type-card\">\n        <div class=\"sb-ccm2-type-icon\">\ud83d\udd2c<\/div>\n        <h3>Feed Supplements<\/h3>\n        <p>Concentrated feed supplements matched to your basal medium. Optimised for fed-batch and perfusion processes to extend culture duration and improve titre.<\/p>\n        <div class=\"sb-ccm2-type-tags\"><span>Fed-batch & perfusion<\/span><span>Liquid & powder formats<\/span><\/div>\n      <\/div>\n      <div class=\"sb-ccm2-type-card\">\n        <div class=\"sb-ccm2-type-icon\">\ud83e\uddec<\/div>\n        <h3>Inhibitor Cocktails<\/h3>\n        <p>Custom small molecule inhibitor supplements formulated to your exact concentrations \u2014 supplied as separate vials for protocol flexibility. Validated for stem cell, organoid and DMPK applications.<\/p>\n        <div class=\"sb-ccm2-type-tags\"><span>Stem cell media<\/span><span>Organoid culture<\/span><\/div>\n      <\/div>\n      <div class=\"sb-ccm2-type-card\">\n        <div class=\"sb-ccm2-type-icon\">\ud83d\udce6<\/div>\n        <h3>Powder Formulations<\/h3>\n        <p>Dry powder media for extended shelf life, reduced cold-chain costs and flexible reconstitution at point of use. Available for most liquid formulations.<\/p>\n        <div class=\"sb-ccm2-type-tags\"><span>Extended shelf life<\/span><span>Reduced shipping cost<\/span><\/div>\n      <\/div>\n    <\/div>\n  <\/div>\n\n  <!-- CELL LINES -->\n  <div class=\"sb-ccm2-section sb-ccm2-alt\">\n    <h2>Cell Line Coverage<\/h2>\n    <p class=\"sb-ccm2-intro\">We develop custom media for all major mammalian cell lines and many specialised systems. If your cell line is not listed, contact us \u2014 we can source and develop for any system.<\/p>\n    <div class=\"sb-ccm2-cl-table-wrap\">\n      <table class=\"sb-ccm2-cl-table\">\n        <thead><tr><th>Cell Line<\/th><th>Primary Application<\/th><th>Media Type<\/th><\/tr><\/thead>\n        <tbody>\n          <tr><td><strong>HEK293 \/ HEK293T \/ HEK293F<\/strong><\/td><td>AAV, lentiviral vectors, recombinant protein<\/td><td>SF, CD, AOF \u2014 adherent & suspension<\/td><\/tr>\n          <tr><td><strong>CHO-K1 \/ CHO-S \/ CHO DG44<\/strong><\/td><td>mAb, therapeutic protein, biosimilar<\/td><td>CD, protein-free \u2014 fed-batch & perfusion<\/td><\/tr>\n          <tr><td><strong>VERO \/ MDCK \/ BHK-21<\/strong><\/td><td>Viral vaccine production<\/td><td>SF, CD \u2014 adherent & microcarrier<\/td><\/tr>\n          <tr><td><strong>Hybridoma \/ Myeloma<\/strong><\/td><td>mAb production for diagnostics<\/td><td>Low-protein SF<\/td><\/tr>\n          <tr><td><strong>T-cells \/ NK cells \/ PBMCs<\/strong><\/td><td>CAR-T, adoptive cell transfer<\/td><td>Xeno-free, GMP-ready<\/td><\/tr>\n          <tr><td><strong>MSCs (bone marrow, adipose, cord)<\/strong><\/td><td>Cell therapy, regenerative medicine<\/td><td>Xeno-free, hPL-supplemented<\/td><\/tr>\n          <tr><td><strong>hPSC \/ iPSC (primed)<\/strong><\/td><td>iPSC expansion, disease modelling<\/td><td>Defined, xeno-free<\/td><\/tr>\n          <tr><td><strong>Naive hPSC<\/strong><\/td><td>Ground state pluripotency, ATMP<\/td><td>NaivaCore (SI + NT components)<\/td><\/tr>\n          <tr><td><strong>Organoids (intestinal, hepatic, brain)<\/strong><\/td><td>Drug screening, disease modelling<\/td><td>OrganoMedium variants<\/td><\/tr>\n          <tr><td><strong>Custom \/ novel cell lines<\/strong><\/td><td>Any application<\/td><td>DoE-based development<\/td><\/tr>\n        <\/tbody>\n      <\/table>\n    <\/div>\n  <\/div>\n\n  <!-- DEVELOPMENT PROCESS -->\n  <div class=\"sb-ccm2-section\">\n    <h2>Development Process<\/h2>\n    <div class=\"sb-ccm2-proc-grid\">\n      <div class=\"sb-ccm2-proc-card\">\n        <div class=\"sb-ccm2-proc-num\">01<\/div>\n        <h3>Consultation<\/h3>\n        <p>Define your cell line, application, target performance specs and regulatory requirements. We assess feasibility, component sourcing and timeline.<\/p>\n        <div class=\"sb-ccm2-proc-time\">Week 1<\/div>\n      <\/div>\n      <div class=\"sb-ccm2-proc-card\">\n        <div class=\"sb-ccm2-proc-num\">02<\/div>\n        <h3>Formulation Design<\/h3>\n        <p>Base medium selection, DoE-based nutrient screening, amino acid and lipid optimisation targeting your specific cell line and process parameters.<\/p>\n        <div class=\"sb-ccm2-proc-time\">Weeks 2\u20136<\/div>\n      <\/div>\n      <div class=\"sb-ccm2-proc-card\">\n        <div class=\"sb-ccm2-proc-num\">03<\/div>\n        <h3>Testing & Validation<\/h3>\n        <p>Growth kinetics, cell viability, productivity, product quality (glycosylation, aggregation) and stability assessment across multiple passages.<\/p>\n        <div class=\"sb-ccm2-proc-time\">Weeks 6\u201312<\/div>\n      <\/div>\n      <div class=\"sb-ccm2-proc-card\">\n        <div class=\"sb-ccm2-proc-num\">04<\/div>\n        <h3>Scale-Up<\/h3>\n        <p>Transfer from bench to bioreactor scale with process characterisation. Performance verification at target production scale.<\/p>\n        <div class=\"sb-ccm2-proc-time\">Weeks 12\u201316<\/div>\n      <\/div>\n      <div class=\"sb-ccm2-proc-card\">\n        <div class=\"sb-ccm2-proc-num\">05<\/div>\n        <h3>GMP Production<\/h3>\n        <p>Commercial manufacturing with full batch documentation, CoA, CoO, stability data. Ongoing supply with batch reservation available.<\/p>\n        <div class=\"sb-ccm2-proc-time\">Month 4\u20136+<\/div>\n      <\/div>\n    <\/div>\n  <\/div>\n\n  <!-- CAPABILITIES -->\n  <div class=\"sb-ccm2-section sb-ccm2-alt\">\n    <h2>What We Offer<\/h2>\n    <div class=\"sb-ccm2-cap-grid\">\n      <div class=\"sb-ccm2-cap-card\">\n        <h3>Flexible Batch Sizes<\/h3>\n        <p>500 mL for early development through to 1000+ litres for commercial supply. No minimum order quantity for standard formulations.<\/p>\n      <\/div>\n      <div class=\"sb-ccm2-cap-card\">\n        <h3>Liquid & Powder<\/h3>\n        <p>Ready-to-use liquid or dry powder for extended shelf life and reduced cold-chain cost. Custom packaging and aliquot sizes available.<\/p>\n      <\/div>\n      <div class=\"sb-ccm2-cap-card\">\n        <h3>Full Documentation<\/h3>\n        <p>CoA, CoO, sterility certificate, endotoxin report, osmolality, pH \u2014 all included. DMF support available for regulatory submissions.<\/p>\n      <\/div>\n      <div class=\"sb-ccm2-cap-card\">\n        <h3>NDA-Protected Formulation<\/h3>\n        <p>Your formulation remains confidential. We produce under signed NDA with full IP protection for client-proprietary recipes.<\/p>\n      <\/div>\n      <div class=\"sb-ccm2-cap-card\">\n        <h3>Batch Reservation<\/h3>\n        <p>Reserve validated production lots for up to 6 weeks at no prepayment cost \u2014 ensuring lot continuity for long-running research or manufacturing programmes.<\/p>\n      <\/div>\n      <div class=\"sb-ccm2-cap-card\">\n        <h3>Technical Support<\/h3>\n        <p>Dedicated project manager and scientific support throughout development, adaptation, scale-up and commercial supply phases.<\/p>\n      <\/div>\n    <\/div>\n  <\/div>\n\n  <!-- FAQ -->\n  <div class=\"sb-ccm2-section\">\n    <h2>Frequently Asked Questions<\/h2>\n    <div class=\"sb-ccm2-faqs\">\n      <div class=\"sb-ccm2-faq\">\n        <h3>How long does custom media development take?<\/h3>\n        <p>Simple modifications to an existing formulation take 2\u20134 weeks. Full custom development from scratch takes 8\u201316 weeks depending on cell line and complexity. GMP-grade production with complete documentation takes 3\u20136 months. A detailed project plan is provided at project start.<\/p>\n      <\/div>\n      <div class=\"sb-ccm2-faq\">\n        <h3>What volumes can you supply?<\/h3>\n        <p>From 500 mL for initial research and development through to 1000+ litres for commercial production. Both liquid and dry powder formats are available. Multi-year supply agreements with volume commitments are available for commercial customers.<\/p>\n      <\/div>\n      <div class=\"sb-ccm2-faq\">\n        <h3>Can you reproduce a media formulation we already have?<\/h3>\n        <p>Yes \u2014 we can reproduce existing formulations including licensed academic recipes (such as HENSM\/NaivaCore), client-developed recipes, or modifications of commercial basal media. We handle all sourcing and QC under NDA.<\/p>\n      <\/div>\n      <div class=\"sb-ccm2-faq\">\n        <h3>Do you offer GMP-grade custom media production?<\/h3>\n        <p>Yes. We produce under ISO 9001 and ISO 13485 conditions with GMP-compatible manufacturing capability. Full regulatory documentation is included \u2014 CoA, CoO, stability data, sterility and endotoxin certificates. DMF\/ASMF support is available for regulatory submissions.<\/p>\n      <\/div>\n    <\/div>\n  <\/div>\n\n  <!-- INTERNAL LINKS -->\n  <div class=\"sb-ccm2-section\">\n    <h2>Ready-Made Media in Our Portfolio<\/h2>\n    <div class=\"sb-ccm2-related\">\n      <a href=\"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/custom-serum-free-media-for-hek293-cho-mammalian-cell-lines\/serum-free-media-for-hek293-cells\/\" class=\"sb-ccm2-rel-card\">\n        <h3>HEK293 Serum-Free Media<\/h3>\n        <p>Defined adherent and suspension media for AAV, lentiviral vector and recombinant protein production.<\/p>\n      <\/a>\n      <a href=\"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/custom-serum-free-media-for-hek293-cho-mammalian-cell-lines\/serum-free-media-for-cho-cells\/\" class=\"sb-ccm2-rel-card\">\n        <h3>CHO Serum-Free Media<\/h3>\n        <p>Chemically defined CHO media for mAb and therapeutic protein production \u2014 fed-batch and perfusion.<\/p>\n      <\/a>\n      <a href=\"https:\/\/www.seamlessbio.de\/products\/cell-culture-media-reagents\/naivacore-naive-stem-cell-medium\/\" class=\"sb-ccm2-rel-card\">\n        <h3>NaivaCore \u2014 Naive Stem Cell Medium<\/h3>\n        <p>Three-component naive hPSC medium for ground state reprogramming and maintenance.<\/p>\n      <\/a>\n      <a href=\"https:\/\/www.seamlessbio.de\/applications\/organomedium\/\" class=\"sb-ccm2-rel-card\">\n        <h3>OrganoMedium<\/h3>\n        <p>Defined organoid culture medium for intestinal, hepatic and multi-tissue organoid expansion.<\/p>\n      <\/a>\n    <\/div>\n  <\/div>\n\n  <!-- CTA -->\n  <div class=\"sb-ccm2-cta\">\n    <h2>Start Your Custom Media Project<\/h2>\n    <p>Tell us your cell line, application and requirements. Our formulation team will respond within 48 hours.<\/p>\n    <div class=\"sb-ccm2-cta-btns\">\n      <a href=\"https:\/\/www.seamlessbio.de\/about-us\/contact-imprint\/\" class=\"sb-ccm2-btn-w\">Contact Us<\/a>\n      <a href=\"mailto:info@seamlessbio.de\" class=\"sb-ccm2-btn-o\">info@seamlessbio.de<\/a>\n    <\/div>\n    <p class=\"sb-ccm2-contact-line\">+49 851 37932226 \u00b7 info@seamlessbio.de<\/p>\n  <\/div>\n\n<\/div>\n<\/section>\n\n<style>\n.sb-ccm2-page, .sb-ccm2-page *, .sb-ccm2-page *::before, .sb-ccm2-page *::after { box-sizing: border-box; }\n.sb-ccm2-page { font-family: Arial, sans-serif; color: #1a1a1a; background: #fff; padding: 60px 32px 90px; }\n.sb-ccm2-container { max-width: 1200px; margin: 0 auto; }\n.sb-ccm2-bc { display: flex; flex-wrap: wrap; gap: 6px; align-items: center; font-size: 13px; color: #888; margin-bottom: 28px; }\n.sb-ccm2-bc a { color: #18b7b2 !important; text-decoration: none !important; font-weight: 600; }\n.sb-ccm2-bc strong { color: #111; }\n.sb-ccm2-bc span { color: #ccc; 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