{"id":9014,"date":"2026-09-25T00:00:00","date_gmt":"2026-09-24T23:00:00","guid":{"rendered":"https:\/\/wheat-quail-856400.hostingersite.com\/?p=9014"},"modified":"2026-09-24T14:59:48","modified_gmt":"2026-09-24T13:59:48","slug":"what-is-animal-component-free-acf-cell-culture","status":"publish","type":"post","link":"https:\/\/seamlessbio.de\/de\/what-is-animal-component-free-acf-cell-culture\/","title":{"rendered":"Was versteht man unter tierkomponentenfreier (ACF) Zellkultur?"},"content":{"rendered":"<p>Tierkomponentenfreie (ACF) Zellkultur bezeichnet die Kultivierung von Zellen mit Medien und Zus\u00e4tzen, die keine Materialien tierischen Ursprungs enthalten \u2013 kein FKS, kein tierisches Serumalbumin und keine Wachstumsfaktoren tierischen Ursprungs. ACF-Systeme werden in der Zelltherapie-Herstellung zunehmend verlangt, weil xenogene Materialien Sicherheits- und Zulassungsrisiken mit sich bringen.<\/p>\n<h2>Warum ACF in der modernen Bioprozesstechnik wichtig ist<\/h2>\n<p>FKS enth\u00e4lt undefinierte Wachstumsfaktoren, Hormone und Proteine, die Variabilit\u00e4t und m\u00f6gliche Kontaminationsrisiken (Mykoplasmen, Viren, Prionen) mit sich bringen. Bei therapeutischen Zellprodukten zur Anwendung am Menschen pr\u00fcfen die Beh\u00f6rden Bestandteile tierischen Ursprungs genau; sie m\u00fcssen minimiert oder beseitigt werden. ACF-Systeme bieten bessere Chargenkonsistenz, bessere R\u00fcckverfolgbarkeit und weniger regulatorischen Aufwand.<\/p>\n<h2>ACF-Alternativen zu FKS<\/h2>\n<p>Wichtige ACF-Alternativen sind humanes Thrombozytenlysat (hPL) f\u00fcr die MSC- und T-Zell-Expansion, rekombinantes humanes Albumin (rHSA) anstelle von BSA, definierte synthetische Medien mit rekombinanten Wachstumsfaktoren sowie chemisch definierte Medien f\u00fcr bestimmte Zelllinien wie CHO und HEK293.<\/p>\n<h2>ACF vs. xenofrei<\/h2>\n<p>\u201eXenofrei\u201c bedeutet, dass keine Materialien einer fremden Spezies verwendet werden (humanes hPL ist also zul\u00e4ssig), w\u00e4hrend \u201eACF\u201c bedeutet, dass \u00fcberhaupt keine Materialien tierischen Ursprungs verwendet werden. F\u00fcr allogene Zelltherapien ist xenofrei in der Regel die Mindestanforderung; ACF ist der strengere Standard.<\/p>\n<p><a href=\"\/de\/zellkultur\/\"><strong>\u2192 Zellkultur-L\u00f6sungen entdecken<\/strong><\/a><\/p>","protected":false},"excerpt":{"rendered":"<p>Tierkomponentenfreie (ACF) Zellkultur verzichtet auf Materialien tierischen Ursprungs. Erfahren Sie, warum ACF f\u00fcr GMP und Zelltherapie wichtig ist und welche Alternativen zu FKS es gibt.<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_templately_pack_id":"","_templately_imported_at":"","_templately_source":"","_templately_import_session_id":"","footnotes":""},"categories":[46],"tags":[],"class_list":["post-9014","post","type-post","status-publish","format-standard","hentry","category-cell-culture"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>What is Animal Component-Free (ACF) Cell Culture? \u2014 Xeno-Free Media Guide<\/title>\n<meta name=\"description\" content=\"Animal component-free (ACF) cell culture eliminates animal-derived materials. 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