{"id":9190,"date":"2026-10-09T21:42:49","date_gmt":"2026-10-09T20:42:49","guid":{"rendered":"https:\/\/wheat-quail-856400.hostingersite.com\/?p=9190"},"modified":"2026-10-27T10:26:27","modified_gmt":"2026-10-27T09:26:27","slug":"hek293t-vs-hek293f-vs-hek293ft-which-serum-free-medium-does-each-need","status":"publish","type":"post","link":"https:\/\/seamlessbio.de\/de\/hek293t-vs-hek293f-vs-hek293ft-which-serum-free-medium-does-each-need\/","title":{"rendered":"HEK293T vs. HEK293F vs. HEK293FT: Welches serumfreie Medium braucht welche Zelllinie?"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"9190\" class=\"elementor elementor-9190\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-59edc8e6 e-flex e-con-boxed e-con e-parent\" data-id=\"59edc8e6\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-58b72f4b elementor-widget elementor-widget-text-editor\" data-id=\"58b72f4b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t\n<p class=\"wp-block-paragraph\">HEK293T vs. HEK293F vs. HEK293FT: Welches serumfreie Medium f\u00fcr welche Linie? | SeamlessBio<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">HEK293 ist nicht eine Zelllinie, sondern eine Familie \u2013 und jedes Mitglied hat eigene Eigenschaften, die die Medienwahl beeinflussen. Ein f\u00fcr eine Sublinie optimiertes Medium bei einer anderen einzusetzen, geh\u00f6rt zu den h\u00e4ufigsten Ursachen f\u00fcr suboptimale Titer in der Herstellung viraler Vektoren.<\/p>\n<!-- BUY BOX (SEO: links to buy pages) -->\n<div class=\"sb-buy-box\" style=\"border:1px solid #cfe9e6;background:#f4fbfa;border-radius:12px;padding:22px 26px;margin:28px auto;max-width:1200px;box-sizing:border-box\">\n<h2 style=\"font-size:22px;line-height:1.3;margin:0 0 6px\">Serumfreie Zellkulturmedien ab EU-Lager kaufen<\/h2>\n<p style=\"margin:0 0 14px\">Angebot oder kostenloses Testmuster anfragen \u2013 keine Mindestbestellmenge, Angebot innerhalb von 48 Stunden.<\/p>\n<ul style=\"margin:0;padding-left:20px;columns:2 300px;column-gap:32px\">\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/simplacex-serumfreies-medium-hek293t\/\">SimplaceX \u2013 serumfreies HEK293T-Medium<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/simplacecdx-chemisch-definiertes-medium-hek293t\/\">SimplaceCDX \u2013 chemisch definiertes HEK293T-Medium<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/naivacore-naives-ipsc-medium-hensm\/\">NaivaCore \u2013 naives iPSC-Medium (HENSM)<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/serumfreies-medium-cho\/\">Serumfreie CHO-Zellkulturmedien<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/cellimax-metacho-cd-cho-basalmedium\/\">CelliMax\u00ae MetaCHO &amp; Versa \u2013 CHO-Basalmedien<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/produkt\/cellimax-cho-fga-feedmedium\/\">CelliMax\u00ae CHO-Feeds \u2013 FGa, FGa plus, FGb<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/serumfreies-msc-medium-gmp\/\">Serumfreies MSC-Medium<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/serumfreies-ipsc-medium-gmp\/\">Serumfreies iPSC- &amp; hESC-Medium<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/anwendungen\/organoid-3d-cell-culture\/\">OrganoMedium \u2013 Medium f\u00fcr die Organoid-Expansion<\/a><\/li>\n<li style=\"margin:0 0 6px\"><a href=\"https:\/\/seamlessbio.de\/de\/massgeschneiderte-zellkulturmedien\/\">Ma\u00dfgeschneiderte Zellkulturmedien \u2013 individuelle Formulierung<\/a><\/li>\n<\/ul>\n<p style=\"margin:14px 0 0\"><a href=\"https:\/\/seamlessbio.de\/de\/request-quote\/\" style=\"font-weight:600\">Angebot anfordern \u2192<\/a><\/p>\n<\/div>\n<!-- \/BUY BOX -->\n\n\n\n\n<p class=\"wp-block-paragraph\">Hier erfahren Sie, was Sie \u00fcber die wichtigsten HEK293-Varianten und ihre Anforderungen an serumfreie Medien wissen m\u00fcssen.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">HEK293 (Ausgangslinie)<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Die urspr\u00fcngliche Zelllinie, 1973 aus humanen embryonalen Nierenzellen gewonnen und mit Adenovirus-5-DNA transformiert. Adh\u00e4rent, m\u00e4\u00dfige Wachstumsrate, gut charakterisiert.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Anforderungen an das Medium:<\/strong> Chemisch definierte Standardformulierung, die adh\u00e4rente Kultur unterst\u00fctzt. Unter richtig formulierten serumfreien Bedingungen ist die Transfektionseffizienz mit PEI hoch. Geeignet f\u00fcr die AAV-Produktion in fr\u00fchen Phasen und die Expression rekombinanter Proteine.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">HEK293T<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">HEK293T tr\u00e4gt eine stabile Integration des SV40-Large-T-Antigens. Dadurch k\u00f6nnen Plasmide mit SV40-Replikationsursprung episomal repliziert werden \u2013 das steigert die transiente Proteinexpression deutlich und ist die Grundlage der hocheffizienten Triple-Plasmid-Transfektion f\u00fcr die Produktion von AAV- und lentiviralen Vektoren.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">HEK293T ist adh\u00e4rent und l\u00e4sst sich ohne erheblichen Selektions- und Adaptationsaufwand nur schlecht an Suspensionskultur gew\u00f6hnen.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Anforderungen an das Medium:<\/strong> F\u00fcr adh\u00e4rente Kultur optimiert. Ein niedriger Albumingehalt ist wichtig \u2013 Albumin st\u00f6rt die Bildung und Aufnahme von PEI-DNA-Komplexen. Das Medium muss eine sehr hohe Konfluenz (70\u201380 % zum Zeitpunkt der Transfektion) unterst\u00fctzen, ohne fr\u00fche Seneszenz auszul\u00f6sen. Entscheidend ist die Viabilit\u00e4t in den 48\u201372 Stunden nach der Transfektion.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">HEK293F<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">HEK293F ist eine an Suspension adaptierte HEK293-Variante ohne SV40-T-Antigen. Sie ist die bevorzugte Zelllinie f\u00fcr die gro\u00dftechnische Expression rekombinanter Proteine und wird zunehmend f\u00fcr AAV-Produktionsplattformen in Suspension genutzt.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Anforderungen an das Medium:<\/strong> Speziell f\u00fcr Suspensionskultur formuliert. Wachstum bis zu hohen Dichten \u2013 kommerziell optimierte HEK293F-Medien erreichen routinem\u00e4\u00dfig lebende Zelldichten \u00fcber 3\u20135 \u00d7 10\u2076 Zellen\/ml. Wichtig ist die Toleranz gegen\u00fcber R\u00fchren: Das Medium muss die Viabilit\u00e4t unter den Scherbedingungen in Spinnerflaschen, Sch\u00fcttelkolben und R\u00fchrkesselbioreaktoren erhalten.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">HEK293F ben\u00f6tigt von Anfang an ein serumfreies Suspensionsmedium. Eine schrittweise FKS-Reduktion in einem Medium f\u00fcr adh\u00e4rente Kultur f\u00fchrt meist zu schlechtem Wachstum und hohem Zelltod.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">HEK293FT<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">HEK293FT verbindet das SV40-T-Antigen von HEK293T mit einer Selektion auf schnelles Wachstum und hohe Transfektionseffizienz. Sie ist die Standardzelllinie f\u00fcr die Produktion lentiviraler Vektoren mit hohem Titer.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Anforderungen an das Medium:<\/strong> \u00c4hnlich wie HEK293T \u2013 adh\u00e4rentes Format, wenig Albumin, hohe Konfluenz vor der Transfektion. Durch die zus\u00e4tzliche Selektion auf schnelles Wachstum k\u00f6nnen HEK293FT-Kulturen bei der Transfektion dichter sein als HEK293T; das Medium muss daher in der Produktionsphase einen etwas h\u00f6heren Stoffwechselbedarf abdecken.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">HEK293-EBNA<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Tr\u00e4gt eine stabile Integration von EBNA-1, die die episomale Replikation von Plasmiden mit EBV-Replikationsursprung erm\u00f6glicht. Wird vor allem f\u00fcr die transiente Expression sezernierter Proteine eingesetzt.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Anforderungen an das Medium:<\/strong> Die EBNA-1-Expression erh\u00f6ht die Stoffwechsellast. Medien f\u00fcr HEK293-EBNA sollten sowohl adh\u00e4rente Kultur als auch die fr\u00fche Adaptation an Suspension unterst\u00fctzen, da viele EBNA-Protokolle ein Scale-up in Suspension vorsehen.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Zusammenfassung<\/h2>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th>Sublinie<\/th><th>T-Antigen<\/th><th>Kulturformat<\/th><th>Hauptanwendung<\/th><th>Wichtigste Anforderung an das Medium<\/th><\/tr><\/thead><tbody><tr><td>HEK293<\/td><td>Nein<\/td><td>Anh\u00e4nger<\/td><td>AAV, Proteinexpression<\/td><td>Standard-CD, albuminarm<\/td><\/tr><tr><td>HEK293T<\/td><td>SV40<\/td><td>Anh\u00e4nger<\/td><td>AAV, lentivirale Vektoren<\/td><td>Albuminarm, unterst\u00fctzt hohe Konfluenz<\/td><\/tr><tr><td>HEK293F<\/td><td>Nein<\/td><td>Aussetzung<\/td><td>Scale-up Protein, AAV<\/td><td>Hohe Dichte, r\u00fchrtolerant<\/td><\/tr><tr><td>HEK293FT<\/td><td>SV40<\/td><td>Anh\u00e4nger<\/td><td>Lentiviral mit hohem Titer<\/td><td>Unterst\u00fctzt schnelles Wachstum, albuminarm<\/td><\/tr><tr><td>HEK293-EBNA<\/td><td>EBNA-1<\/td><td>Adh\u00e4rent\/Suspension<\/td><td>Transient sezernierte Proteine<\/td><td>EBNA-1-kompatibel, skalierbar<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\u2192 SeamlessBio liefert serumfreie Medien f\u00fcr HEK293-Zellen in adh\u00e4renten und Suspensionsformaten, mit Optionen f\u00fcr die AAV-Produktion, die Herstellung lentiviraler Vektoren und die Expression rekombinanter Proteine: <a href=\"\/de\/serumfreies-medium-hek293\/\">Serumfreie Medien f\u00fcr HEK293-Zellen<\/a><\/strong><\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>HEK293T vs. HEK293F vs. HEK293FT: Which Serum-Free Medium for Each? | SeamlessBio HEK293 is not one cell line. It is a family \u2014 and each member has distinct characteristics that affect medium selection. Using a formulation optimised for one sub-line on another is one of the most common sources of suboptimal titre in viral vector [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_templately_pack_id":"","_templately_imported_at":"","_templately_source":"","_templately_import_session_id":"","footnotes":""},"categories":[46,256],"tags":[],"class_list":["post-9190","post","type-post","status-publish","format-standard","hentry","category-cell-culture","category-serum-free"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>HEK293: Which Serum-Free Medium Does Each Need?<\/title>\n<meta name=\"description\" content=\"HEK293T, HEK293F and HEK293FT are not interchangeable. Here&#039;s what each sub-line needs from a serum-free medium and why the wrong choice limits your viral vector titre.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/seamlessbio.de\/de\/hek293t-vs-hek293f-vs-hek293ft-which-serum-free-medium-does-each-need\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"HEK293: Which Serum-Free Medium Does Each Need?\" \/>\n<meta property=\"og:description\" content=\"HEK293T, HEK293F and HEK293FT are not interchangeable. 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