{"id":3542,"date":"2026-09-16T12:09:52","date_gmt":"2026-09-16T11:09:52","guid":{"rendered":"https:\/\/seamlessbio.de\/produkt\/human-pgp-vesicle-kit-with-fluopgp-substrate-hek293-derived-fluorescence-pgp-assay-100-reactions\/"},"modified":"2026-09-21T15:07:59","modified_gmt":"2026-09-21T14:07:59","slug":"human-pgp-vesicle-kit-with-fluopgp-substrate-hek293-derived-fluorescence-pgp-assay-100-reactions","status":"publish","type":"product","link":"https:\/\/seamlessbio.de\/de\/product\/human-pgp-vesicle-kit-with-fluopgp-substrate-hek293-derived-fluorescence-pgp-assay-100-reactions\/","title":{"rendered":"Human Pgp Vesicle Kit with FluoPgp Substrate (HEK293 derived) | Fluorescence Pgp Assay | 100 Reactions"},"content":{"rendered":"<style>\n.sb-pd{font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",Roboto,sans-serif;max-width:100%;color:#202020;line-height:1.75}\n.sb-pd .def{background:#e9fbfa;border-left:4px solid #18b7b2;border-radius:0 10px 10px 0;padding:16px 20px;margin:0 0 28px}\n.sb-pd .def .lb{font-size:11px;font-weight:800;text-transform:uppercase;letter-spacing:.09em;color:#139b97;display:block;margin-bottom:5px}\n.sb-pd .def p{font-size:15px;margin:0;font-weight:500}\n.sb-pd h2{font-size:21px;font-weight:800;margin:32px 0 10px;color:#111;border-bottom:2px solid #e2eceb;padding-bottom:7px}\n.sb-pd h3{font-size:15px;font-weight:700;color:#18b7b2;margin:18px 0 5px}\n.sb-pd p{margin:0 0 12px;font-size:15px}\n.sb-pd ul{margin:0 0 12px;padding-left:20px}\n.sb-pd li{margin-bottom:6px;font-size:14px}\n.sb-pd .cards{display:grid;grid-template-columns:repeat(3,1fr);gap:12px;margin:14px 0 20px}\n.sb-pd .card{background:#f1faf9;border:1px solid #daf3f2;border-top:3px solid #18b7b2;border-radius:0 0 10px 10px;padding:14px}\n.sb-pd .card h3{margin:0 0 5px;font-size:13px;color:#111}\n.sb-pd .card p{margin:0;font-size:12px;color:#5b6b71}\n.sb-pd table{width:100%;border-collapse:collapse;font-size:14px;margin:14px 0 20px}\n.sb-pd th{background:#18b7b2;color:#fff;text-align:left;padding:9px 13px;font-weight:700}\n.sb-pd td{padding:9px 13px;border-bottom:1px solid #e2eceb;vertical-align:top}\n.sb-pd tr:nth-child(even) td{background:#f8fffe}\n.sb-pd td:first-child{font-weight:600;color:#111;width:35%}\n.sb-pd .qa{background:#f8fffe;border:1px solid #daf3f2;border-radius:8px;padding:14px 16px;margin:10px 0}\n.sb-pd .qa .q{font-weight:700;color:#111;margin:0 0 5px;font-size:14px}\n.sb-pd .qa .a{margin:0;font-size:13px;color:#444}\n.sb-pd .cta{background:linear-gradient(135deg,#e4f6f5,#f4fbfb);border:1px solid rgba(24,183,178,.2);border-radius:12px;padding:20px;margin-top:28px;text-align:center}\n.sb-pd .cta h3{margin:0 0 7px;font-size:17px;color:#111}\n.sb-pd .cta p{margin:0 0 14px;font-size:13px;color:#5b6b71}\n.sb-pd .btn{display:inline-block;background:#18b7b2;color:#fff!important;padding:9px 20px;border-radius:7px;font-weight:700;font-size:13px;text-decoration:none!important;margin:0 4px}\n.sb-pd .btn-o{display:inline-block;border:2px solid #18b7b2;color:#18b7b2!important;padding:7px 20px;border-radius:7px;font-weight:700;font-size:13px;text-decoration:none!important;margin:0 4px}\n.sb-pd a{color:#18b7b2;font-weight:600}\n@media(max-width:600px){.sb-pd .cards{grid-template-columns:1fr}}\n<\/style>\n<div class=\"sb-pd\">\n<h2>Product Overview<\/h2>\n<p>The <strong>Human P-gp + FluoPgp Vesicle Kit (HEK293)<\/strong> combines the proven Cell4Pharma P-glycoprotein inside-out vesicle system with the proprietary FluoPgp fluorescent probe substrate \u2014 eliminating the need for radiolabelled compounds or LC-MS\/MS infrastructure. The result is a fast, plate reader-compatible P-gp transport assay suitable for high-throughput screening (HTS), compound library profiling, and laboratories without radiolabel or mass spectrometry capability.<\/p>\n<p>P-glycoprotein (ABCB1 \/ MDR1) is a Tier 1 DDI transporter under FDA DDI Guidance (2020) and EMA DDI Guideline (2013\/2022) \u2014 substrate and inhibitor assessment is a regulatory requirement for all new drug candidates. This kit generates IC\u2085\u2080 data with full regulatory validity using fluorescence detection, with no change to the underlying vesicular transport assay principle. Use alongside the <a href=\"https:\/\/seamlessbio.de\/abc-transporter-vesicle-kits\/\">Human Control Vesicle Kit<\/a> for matched background subtraction. If radiolabel or LC-MS\/MS detection is preferred, the <a href=\"https:\/\/seamlessbio.de\/abc-transporter-vesicle-kits\/\">Human P-gp Vesicle Kit<\/a> is available separately.<\/p>\n<h2>Technical Specifications<\/h2>\n<table>\n<thead>\n<tr>\n<th>Parameter<\/th>\n<th>Specification<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Transporter<\/td>\n<td>Human P-gp \/ MDR1 (ABCB1)<\/td>\n<\/tr>\n<tr>\n<td>Expression system<\/td>\n<td>HEK293 (transient overexpression)<\/td>\n<\/tr>\n<tr>\n<td>Vesicle type<\/td>\n<td>Inside-out membrane vesicles<\/td>\n<\/tr>\n<tr>\n<td>Probe substrate<\/td>\n<td>FluoPgp \u2014 proprietary Cell4Pharma fluorescent P-gp substrate<\/td>\n<\/tr>\n<tr>\n<td>Reactions per kit<\/td>\n<td>100<\/td>\n<\/tr>\n<tr>\n<td>ATP\/AMP transport ratio<\/td>\n<td>&gt;5 (guaranteed per lot)<\/td>\n<\/tr>\n<tr>\n<td>Detection method<\/td>\n<td>Fluorescence plate reader \u2014 no radiolabel or LC-MS\/MS required<\/td>\n<\/tr>\n<tr>\n<td>Storage<\/td>\n<td>\u221280 \u00b0C (supplied on dry ice)<\/td>\n<\/tr>\n<tr>\n<td>Kit contents<\/td>\n<td>Vesicles, FluoPgp substrate, ATP, AMP, reaction buffer, wash buffer, CoA<\/td>\n<\/tr>\n<tr>\n<td>Manufacturer<\/td>\n<td>Cell4Pharma (Netherlands)<\/td>\n<\/tr>\n<tr>\n<td>Distributor DACH<\/td>\n<td>SeamlessBio GmbH, Passau<\/td>\n<\/tr>\n<tr>\n<td>Regulatory<\/td>\n<td>Research Use Only (RUO)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>Applications<\/h2>\n<table>\n<thead>\n<tr>\n<th>Application<\/th>\n<th>Description<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td><strong>High-throughput P-gp screening (HTS)<\/strong><\/td>\n<td>Screen large compound libraries for P-gp inhibition potential using fluorescence readout. FluoPgp is compatible with all standard fluorescence plate readers and automation-friendly liquid handling systems \u2014 no scintillation counter, no mass spectrometer, no radiolabel.<\/td>\n<\/tr>\n<tr>\n<td><strong>Regulatory P-gp DDI assessment without radiolabel<\/strong><\/td>\n<td>Generate IC\u2085\u2080 values for FDA\/EMA DDI submission using fluorescence detection. Regulatory validity is determined by assay conditions and ATP\/AMP ratio, not by detection method \u2014 fluorescence data from a validated vesicular transport assay with appropriate controls is accepted.<\/td>\n<\/tr>\n<tr>\n<td><strong>Laboratories without radiolabel infrastructure<\/strong><\/td>\n<td>Complete P-gp transport assay with full regulatory data output, accessible to any laboratory equipped with a fluorescence plate reader. No radioactive waste, no special handling permits or licensed facilities required.<\/td>\n<\/tr>\n<tr>\n<td><strong>Compound library profiling and early-phase screening<\/strong><\/td>\n<td>Cost-efficient P-gp substrate and inhibitor profiling during lead optimisation, before committing to radiolabel or LC-MS\/MS confirmatory assays. The assay format supports parallel screening of large compound sets.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>Assay Principle<\/h2>\n<p>Inside-out membrane vesicles present the cytoplasmic face of P-gp to the external assay buffer, allowing ATP-dependent uptake of the FluoPgp substrate into the vesicle lumen. Vesicles are incubated with test compound and FluoPgp in the presence of ATP (active transport condition) or AMP (background control). The reaction is stopped with cold wash buffer; vesicles are separated by rapid filtration and intravesicular FluoPgp fluorescence is quantified directly on a plate reader. The ATP\/AMP fluorescence ratio \u2014 calculated against matched Human Control Vesicles \u2014 provides the assay window for IC\u2085\u2080 determination. The assay principle is identical to the standard radiolabel vesicular transport format; only the detection step differs.<\/p>\n<h2>Complete Cell4Pharma Kit Portfolio<\/h2>\n<p>Order all ABC transporter vesicle kits from a single EU source \u2014 fast cold-chain delivery, guaranteed ATP\/AMP ratios, full lot documentation.<\/p>\n<table>\n<thead>\n<tr>\n<th>Kit<\/th>\n<th>Transporter<\/th>\n<th>Gene<\/th>\n<th>ATP\/AMP Ratio<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Human BCRP Vesicle Kit<\/td>\n<td>BCRP<\/td>\n<td>ABCG2<\/td>\n<td>&gt;10<\/td>\n<\/tr>\n<tr>\n<td>Human BSEP Vesicle Kit<\/td>\n<td>BSEP<\/td>\n<td>ABCB11<\/td>\n<td>&gt;10<\/td>\n<\/tr>\n<tr>\n<td>Human MRP1 Vesicle Kit<\/td>\n<td>MRP1<\/td>\n<td>ABCC1<\/td>\n<td>&gt;5<\/td>\n<\/tr>\n<tr>\n<td>Human MRP2 Vesicle Kit<\/td>\n<td>MRP2<\/td>\n<td>ABCC2<\/td>\n<td>&gt;20<\/td>\n<\/tr>\n<tr>\n<td>Human MRP3 Vesicle Kit<\/td>\n<td>MRP3<\/td>\n<td>ABCC3<\/td>\n<td>&gt;5<\/td>\n<\/tr>\n<tr>\n<td>Human MRP4 Vesicle Kit<\/td>\n<td>MRP4<\/td>\n<td>ABCC4<\/td>\n<td>&gt;5<\/td>\n<\/tr>\n<tr>\n<td>Human MRP5 Vesicle Kit<\/td>\n<td>MRP5<\/td>\n<td>ABCC5<\/td>\n<td>&gt;5<\/td>\n<\/tr>\n<tr>\n<td>Human MRP8 Vesicle Kit<\/td>\n<td>MRP8<\/td>\n<td>ABCC11<\/td>\n<td>&gt;5<\/td>\n<\/tr>\n<tr>\n<td>Human P-gp Vesicle Kit<\/td>\n<td>P-gp \/ MDR1<\/td>\n<td>ABCB1<\/td>\n<td>&gt;5<\/td>\n<\/tr>\n<tr>\n<td><strong>Human P-gp + FluoPgp Kit<\/strong><\/td>\n<td>P-gp \/ MDR1<\/td>\n<td>ABCB1<\/td>\n<td>&gt;5<\/td>\n<\/tr>\n<tr>\n<td>Human Control Vesicle Kit<\/td>\n<td>\u2014<\/td>\n<td>HEK293 empty vector<\/td>\n<td>\u2014<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>Available Formats<\/h2>\n<table>\n<thead>\n<tr>\n<th>Format<\/th>\n<th>Reactions<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Kit<\/td>\n<td>100 reactions<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>Key Benefits<\/h2>\n<div class=\"cards\">\n<div class=\"card\">\n<h3>Batch Reservation<\/h3>\n<p>Reserve validated lots up to 36 months for reproducible long-term DDI screening workflows.<\/p>\n<\/div>\n<div class=\"card\">\n<h3>Full Documentation<\/h3>\n<p>Lot-specific QC report and data sheet included with every kit \u2014 from ISO 9001 and ISO 13485 certified partner facilities.<\/p>\n<\/div>\n<div class=\"card\">\n<h3>EU &amp; Swiss Supply<\/h3>\n<p>Manufactured by Cell4Pharma (Netherlands) \u2014 fast cold-chain delivery across EU, UK and Switzerland.<\/p>\n<\/div>\n<\/div>\n<h2>Frequently Asked Questions<\/h2>\n<div class=\"qa\">\n<p class=\"q\">Is the FluoPgp fluorescence assay accepted by FDA and EMA for DDI submissions?<\/p>\n<p class=\"a\">Yes \u2014 regulatory acceptance of a vesicular transport assay depends on the demonstrated ATP\/AMP transport ratio (&gt;5), appropriate positive and negative controls, and validated assay conditions, not on the detection method. Fluorescence data from a validated vesicular transport assay using FluoPgp, with the required control materials included in this kit, meets the evidentiary standard for DDI submissions under FDA DDI Guidance (2020) and EMA DDI Guideline (2013\/2022). The lot-specific QC report and data sheet supplied with every kit document the ATP\/AMP ratio and assay performance required for regulatory packages.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">What is the FluoPgp substrate, and how does it differ from standard radiolabelled P-gp substrates?<\/p>\n<p class=\"a\">FluoPgp is a proprietary Cell4Pharma fluorescent probe substrate that is transported by P-glycoprotein (ABCB1) in an ATP-dependent manner. It functions identically to classical radiolabelled P-gp substrates such as [\u00b3H]-vinblastine or [\u00b3H]-digoxin in the vesicular transport assay \u2014 ATP-dependent accumulation in vesicles is quantified and compared between ATP and AMP conditions. The difference is detection only: FluoPgp fluorescence is read directly on a standard plate reader, eliminating the need for a scintillation counter, LC-MS\/MS system, radiolabelled compound handling, and radioactive waste disposal. Assay throughput and data quality are equivalent to radiolabel-based formats.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">Can the FluoPgp substrate be used with existing P-gp vesicles from another source?<\/p>\n<p class=\"a\">The FluoPgp substrate is supplied as an integrated component of this combined kit and is validated specifically against the Cell4Pharma P-gp vesicle preparation. Compatibility with vesicles from other sources has not been tested and cannot be guaranteed. If you already have Cell4Pharma P-gp vesicles and wish to add FluoPgp fluorescence capability to an existing workflow, please contact us to discuss options. For new projects starting from scratch, the combined P-gp + FluoPgp Kit provides the simplest and most fully validated path to fluorescence-based P-gp DDI data.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">When should the standard Human P-gp Vesicle Kit be used instead of the FluoPgp kit?<\/p>\n<p class=\"a\">The standard Human P-gp Vesicle Kit (without FluoPgp) is preferred when radiolabel or LC-MS\/MS detection is already established in the laboratory and preferred for your regulatory submission package, when the test compound is fluorescent and would interfere with the FluoPgp fluorescence signal, or when an existing validated SOP specifies a non-fluorescence detection method. For all other cases \u2014 including high-throughput screening, early-phase profiling, and laboratories without radiolabel infrastructure \u2014 the FluoPgp kit offers a simpler and faster route to the same regulatory-grade P-gp transport data.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">What documentation is supplied with the Human P-gp + FluoPgp Vesicle Kit?<\/p>\n<p class=\"a\">Every kit shipment includes a lot-specific QC report confirming the ATP\/AMP transport ratio (&gt;5) with the FluoPgp substrate, and a data sheet with recommended assay conditions, FluoPgp concentrations, and reference inhibitor controls (e.g. elacridar \/ GF120918, verapamil). All Cell4Pharma kits are manufactured under ISO 9001 and ISO 13485 certified quality systems. Batch reservation for up to 36 months is available on request, ensuring lot-to-lot consistency in the FluoPgp substrate and vesicle preparation across extended screening campaigns.<\/p>\n<\/div>\n<div class=\"cta\">\n<h3>Request a Quote or Sample<\/h3>\n<p>48h response \u00b7 EU &amp; Swiss Supply \u00b7 Batch reservation available \u00b7 Full documentation<\/p>\n<p>  <a href=\"https:\/\/seamlessbio.de\/request-quote\/\" class=\"btn\">Request Quote \u2192<\/a><br \/>\n  <a href=\"https:\/\/seamlessbio.de\/abc-transporter-vesicle-kits\/\" class=\"btn-o\">All ABC Transporter Kits \u2192<\/a>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Human Pgp Vesicle Kit with FluoPgp Substrate (HEK293 derived) | Fluorescence Pgp Assay | 100 Reactions. Available in 100. Price on request \u2014 contact our team for availability, batch documentation and delivery. <\/p>\n<div style='margin-top:12px'><a href='https:\/\/seamlessbio.de\/de\/sammelreservierung\/' style='display:block;background:#fff;border:2px solid #18b7b2;color:#18b7b2;padding:10px 24px;border-radius:8px;font-weight:700;font-size:14px;text-decoration:none;text-align:center;'>Test Sample &amp; Batch Reservation \u2192<\/a><\/div>","protected":false},"featured_media":3704,"template":"","meta":{"_eb_attr":""},"product_brand":[],"product_cat":[73],"product_tag":[79,80,81,82,83],"class_list":["post-3542","product","type-product","status-publish","has-post-thumbnail","product_cat-dmpk-transporter-kits","product_tag-fluopgp-substrate","product_tag-fluorescence-pgp-assay","product_tag-hek293-derived-vesicles","product_tag-human-pgp-vesicle-kit","product_tag-pgp-activity-testing","first","instock","taxable","shipping-taxable","product-type-simple"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>P-gp FluoPgp Vesicle Kit | Non-Radioactive | SeamlessBio<\/title>\n<meta name=\"description\" content=\"P-gp FluoPgp HEK293 vesicle kit with fluorescent substrate. 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