{"id":3548,"date":"2026-09-16T12:09:54","date_gmt":"2026-09-16T11:09:54","guid":{"rendered":"https:\/\/seamlessbio.de\/produkt\/human-mrp1-vesicle-kit-hek293-derived-abcc1-ddi-transporter-assay-100-reactions\/"},"modified":"2026-09-22T21:48:49","modified_gmt":"2026-09-22T20:48:49","slug":"human-mrp1-vesicle-kit-hek293-derived-abcc1-ddi-transporter-assay-100-reactions","status":"publish","type":"product","link":"https:\/\/seamlessbio.de\/de\/product\/human-mrp1-vesicle-kit-hek293-derived-abcc1-ddi-transporter-assay-100-reactions\/","title":{"rendered":"Human MRP1 Vesicle Kit (HEK293 derived) | ABCC1"},"content":{"rendered":"<style>\n.sb-pd{font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",Roboto,sans-serif;max-width:100%;color:#202020;line-height:1.75}\n.sb-pd .def{background:#e9fbfa;border-left:4px solid #18b7b2;border-radius:0 10px 10px 0;padding:16px 20px;margin:0 0 28px}\n.sb-pd .def .lb{font-size:11px;font-weight:800;text-transform:uppercase;letter-spacing:.09em;color:#139b97;display:block;margin-bottom:5px}\n.sb-pd .def p{font-size:15px;margin:0;font-weight:500}\n.sb-pd h2{font-size:21px;font-weight:800;margin:32px 0 10px;color:#111;border-bottom:2px solid #e2eceb;padding-bottom:7px}\n.sb-pd h3{font-size:15px;font-weight:700;color:#18b7b2;margin:18px 0 5px}\n.sb-pd p{margin:0 0 12px;font-size:15px}\n.sb-pd ul{margin:0 0 12px;padding-left:20px}\n.sb-pd li{margin-bottom:6px;font-size:14px}\n.sb-pd .cards{display:grid;grid-template-columns:repeat(3,1fr);gap:12px;margin:14px 0 20px}\n.sb-pd .card{background:#f1faf9;border:1px solid #daf3f2;border-top:3px solid #18b7b2;border-radius:0 0 10px 10px;padding:14px}\n.sb-pd .card h3{margin:0 0 5px;font-size:13px;color:#111}\n.sb-pd .card p{margin:0;font-size:12px;color:#5b6b71}\n.sb-pd table{width:100%;border-collapse:collapse;font-size:14px;margin:14px 0 20px}\n.sb-pd th{background:#18b7b2;color:#fff;text-align:left;padding:9px 13px;font-weight:700}\n.sb-pd td{padding:9px 13px;border-bottom:1px solid #e2eceb;vertical-align:top}\n.sb-pd tr:nth-child(even) td{background:#f8fffe}\n.sb-pd td:first-child{font-weight:600;color:#111;width:35%}\n.sb-pd .qa{background:#f8fffe;border:1px solid #daf3f2;border-radius:8px;padding:14px 16px;margin:10px 0}\n.sb-pd .qa .q{font-weight:700;color:#111;margin:0 0 5px;font-size:14px}\n.sb-pd .qa .a{margin:0;font-size:13px;color:#444}\n.sb-pd .cta{background:linear-gradient(135deg,#e4f6f5,#f4fbfb);border:1px solid rgba(24,183,178,.2);border-radius:12px;padding:20px;margin-top:28px;text-align:center}\n.sb-pd .cta h3{margin:0 0 7px;font-size:17px;color:#111}\n.sb-pd .cta p{margin:0 0 14px;font-size:13px;color:#5b6b71}\n.sb-pd .btn{display:inline-block;background:#18b7b2;color:#fff!important;padding:9px 20px;border-radius:7px;font-weight:700;font-size:13px;text-decoration:none!important;margin:0 4px}\n.sb-pd .btn-o{display:inline-block;border:2px solid #18b7b2;color:#18b7b2!important;padding:7px 20px;border-radius:7px;font-weight:700;font-size:13px;text-decoration:none!important;margin:0 4px}\n.sb-pd a{color:#18b7b2;font-weight:600}\n@media(max-width:600px){.sb-pd .cards{grid-template-columns:1fr}}\n<\/style>\n<div class=\"sb-pd\">\n<div class=\"def\"><span class=\"lb\">What is MRP1?<\/span><\/p>\n<p>MRP1 (ABCC1) is an ATP-binding cassette efflux transporter broadly expressed in lung, testis, kidney, blood-brain barrier, and immune cells. It mediates ATP-dependent export of glutathione conjugates, glucuronides, sulfate conjugates, and anticancer drugs \u2014 making it a critical transporter in oncology DDI studies and tissue distribution profiling.<\/p>\n<\/div>\n<h2>Human MRP1 Vesicle Kit &ndash; Inside-Out Membrane Vesicles for Multidrug Resistance and Organic Anion Transport Studies<\/h2>\n<p>The Human MRP1 Vesicle Kit (HEK293-derived) from Cell4Pharma provides ready-to-use inside-out membrane vesicles for ATP-dependent transport studies of Multidrug Resistance-Associated Protein 1 (MRP1 \/ ABCC1). Each kit delivers 100 reactions with a guaranteed ATP\/AMP ratio of &gt;5. Available exclusively in DACH through SeamlessBio &mdash; fast EU cold-chain delivery from Passau, Germany.<\/p>\n<h3>Transporter Profile<\/h3>\n<p>MRP1 (ABCC1) is broadly expressed in lung, testis, kidney, blood-brain barrier, cardiac and skeletal muscle, placenta, and macrophages. It transports glutathione (GSH), glutathione conjugates (GS-X), glucuronides, sulfate conjugates, and lipophilic drugs complexed with glutathione. Key substrates: E217&beta;G, GSSG, saquinavir, ritonavir, methotrexate, daunorubicin, leukotrienes. Known inhibitors: MK-571, probenecid, indomethacin.<\/p>\n<h3>Technische Daten<\/h3>\n<table>\n<tr>\n<th>Parameter<\/th>\n<th>Spezifikation<\/th>\n<\/tr>\n<tr>\n<td>Transporter<\/td>\n<td>Human MRP1 (ABCC1)<\/td>\n<\/tr>\n<tr>\n<td>Expressionssystem<\/td>\n<td>HEK293 (transient overexpression)<\/td>\n<\/tr>\n<tr>\n<td>Vesicle type<\/td>\n<td>Inside-out membrane vesicles<\/td>\n<\/tr>\n<tr>\n<td>Reaktionen pro Kit<\/td>\n<td>100<\/td>\n<\/tr>\n<tr>\n<td>ATP\/AMP transport ratio<\/td>\n<td>&gt;5<\/td>\n<\/tr>\n<tr>\n<td>Detection compatibility<\/td>\n<td>Radiolabel (&sup3;H-E217&beta;G), LC-MS\/MS, fluorescence<\/td>\n<\/tr>\n<tr>\n<td>Lagerung<\/td>\n<td>&minus;80&nbsp;&deg;C (supplied on dry ice)<\/td>\n<\/tr>\n<tr>\n<td>Kit contents<\/td>\n<td>Vesicles, ATP, AMP, reaction buffer, wash buffer, CoA<\/td>\n<\/tr>\n<tr>\n<td>Manufacturer<\/td>\n<td>Cell4Pharma (Netherlands)<\/td>\n<\/tr>\n<tr>\n<td>DACH-Vertriebspartner<\/td>\n<td>SeamlessBio GmbH, Passau, Germany<\/td>\n<\/tr>\n<\/table>\n<h3>Anwendungen<\/h3>\n<table>\n<tr>\n<th>Anwendung<\/th>\n<th>Description<\/th>\n<\/tr>\n<tr>\n<td><strong>Multidrug resistance (MDR) profiling<\/strong><\/td>\n<td>Assess whether anticancer compounds are MRP1 substrates &mdash; relevant for tumour drug efflux, resistance mechanisms, and combination therapy design.<\/td>\n<\/tr>\n<tr>\n<td><strong>Glutathione\/glucuronide conjugate transport<\/strong><\/td>\n<td>Characterise phase II metabolite efflux via MRP1 &mdash; important for systemic exposure of conjugated drug metabolites.<\/td>\n<\/tr>\n<tr>\n<td><strong>Blood-brain barrier distribution<\/strong><\/td>\n<td>MRP1 at the BBB restricts CNS drug access &mdash; relevant for CNS drug candidates.<\/td>\n<\/tr>\n<tr>\n<td><strong>Tissue DDI profiling<\/strong><\/td>\n<td>Assessment of MRP1-mediated interactions at lung, cardiac, and immune cell sites.<\/td>\n<\/tr>\n<\/table>\n<h3>Assay Principle<\/h3>\n<p>Inside-out membrane vesicles present the cytoplasmic face of the transporter to the assay buffer, enabling ATP-dependent substrate uptake into the vesicle lumen. Incubate with test compound and probe substrate in the presence of ATP (transport) or AMP (background). Stop with cold wash buffer, filter vesicles, and quantify by LC-MS\/MS, radiolabel, or fluorescence. The ATP\/AMP ratio defines the assay window for IC&#8325;&#8320; determination.<\/p>\n<h2>Key Benefits<\/h2>\n<div class=\"cards\">\n<div class=\"card\">\n<h3>Sammelreservierung<\/h3>\n<p>Reserve validated lots up to 24 months for reproducible long-term workflows.<\/p>\n<\/div>\n<div class=\"card\">\n<h3>Vollst\u00e4ndige Dokumentation<\/h3>\n<p>CoA, QC report, data sheet and additional certificates included with every shipment.<\/p>\n<\/div>\n<div class=\"card\">\n<h3>EU Stock<\/h3>\n<p>Warehoused in Passau, Germany &mdash; fast cold-chain delivery across EU, UK and Switzerland.<\/p>\n<\/div>\n<\/div>\n<h3>H\u00e4ufig gestellte Fragen<\/h3>\n<div class=\"qa\">\n<p class=\"q\">Is MRP1 a regulatory requirement for DDI submissions?<\/p>\n<p class=\"a\">MRP1 is not listed as a mandatory Tier 1 transporter by FDA or EMA, but is recommended for oncology compounds, compounds with significant lung\/brain\/cardiac distribution, or extensive phase II metabolites.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">What probe substrate is recommended for MRP1?<\/p>\n<p class=\"a\">E217&beta;G (estradiol-17&beta;-D-glucuronide) is the standard probe &mdash; available as &sup3;H-labelled or unlabelled for LC-MS\/MS. GSSG is also commonly used.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">Can I order MRP1 together with other MRP kits?<\/p>\n<p class=\"a\">Yes &mdash; SeamlessBio supplies the complete Cell4Pharma portfolio (MRP1&ndash;8, P-gp, BCRP, BSEP, Control) from a single EU source with combined cold-chain shipment.<\/p>\n<\/div>\n<div class=\"cta\">\n<h3>Request a Quote or Sample<\/h3>\n<p>48h response &middot; EU Stock &middot; Batch reservation available &middot; Full documentation<\/p>\n<p>  <a href=\"https:\/\/seamlessbio.de\/de\/request-quote\/\" class=\"btn\">Request Quote &rarr;<\/a><br \/>\n  <a href=\"https:\/\/seamlessbio.de\/de\/abc-transporter-vesicle-kits\/\" class=\"btn-o\">All ABC Transporter Kits &rarr;<\/a><br \/>\n  <a href=\"https:\/\/seamlessbio.de\/de\/ressourcen\/blog\/mrp-transporters-guide-mrp1-mrp8-which-kit\/\" class=\"btn-o\">MRP Transporters Guide &rarr;<\/a>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Human MRP1 Vesicle Kit (HEK293 derived) | ABCC1 | DDI Transporter Assay | 100 Reactions. Available in 100. Price on request \u2014 contact our team for availability, batch documentation and delivery. <\/p>\n<div style='margin-top:12px'><a href='https:\/\/seamlessbio.de\/de\/sammelreservierung\/' style='display:block;background:#fff;border:2px solid #18b7b2;color:#18b7b2;padding:10px 24px;border-radius:8px;font-weight:700;font-size:14px;text-decoration:none;text-align:center;'>Test Sample &amp; Batch Reservation \u2192<\/a><\/div>","protected":false},"featured_media":3704,"template":"","meta":{"_eb_attr":""},"product_brand":[252],"product_cat":[73],"product_tag":[58],"class_list":["post-3548","product","type-product","status-publish","has-post-thumbnail","product_brand-cell4pharma","product_cat-dmpk-transporter-kits","product_tag-cell-culture","first","instock","taxable","shipping-taxable","product-type-simple"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>MRP1 Vesicle Kit | ABCC1 DDI Assay | SeamlessBio<\/title>\n<meta name=\"description\" content=\"MRP1 ABCC1 HEK293 inside-out vesicle kit for drug transporter interaction studies. 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