{"id":3598,"date":"2026-09-16T12:10:25","date_gmt":"2026-09-16T11:10:25","guid":{"rendered":"https:\/\/seamlessbio.de\/produkt\/bsa-fatty-acid-free\/"},"modified":"2026-09-19T17:17:32","modified_gmt":"2026-09-19T16:17:32","slug":"bsa-frei-von-fettsauren","status":"publish","type":"product","link":"https:\/\/seamlessbio.de\/de\/product\/bsa-fatty-acid-free\/","title":{"rendered":"BSA Fetts\u00e4urefrei <0.01% | \u226599% Purity | US Origin | Powder"},"content":{"rendered":"<style>\n.sb-pd{font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",Roboto,sans-serif;max-width:100%;color:#202020;line-height:1.75}\n.sb-pd .def{background:#e9fbfa;border-left:4px solid #18b7b2;border-radius:0 10px 10px 0;padding:16px 20px;margin:0 0 28px}\n.sb-pd .def .lb{font-size:11px;font-weight:800;text-transform:uppercase;letter-spacing:.09em;color:#139b97;display:block;margin-bottom:5px}\n.sb-pd .def p{font-size:15px;margin:0;font-weight:500}\n.sb-pd h2{font-size:21px;font-weight:800;margin:32px 0 10px;color:#111;border-bottom:2px solid #e2eceb;padding-bottom:7px}\n.sb-pd h3{font-size:15px;font-weight:700;color:#18b7b2;margin:18px 0 5px}\n.sb-pd p{margin:0 0 12px;font-size:15px}\n.sb-pd ul{margin:0 0 12px;padding-left:20px}\n.sb-pd li{margin-bottom:6px;font-size:14px}\n.sb-pd .cards{display:grid;grid-template-columns:repeat(3,1fr);gap:12px;margin:14px 0 20px}\n.sb-pd .card{background:#f1faf9;border:1px solid #daf3f2;border-top:3px solid #18b7b2;border-radius:0 0 10px 10px;padding:14px}\n.sb-pd .card h3{margin:0 0 5px;font-size:13px;color:#111}\n.sb-pd .card p{margin:0;font-size:12px;color:#5b6b71}\n.sb-pd table{width:100%;border-collapse:collapse;font-size:14px;margin:14px 0 20px}\n.sb-pd th{background:#18b7b2;color:#fff;text-align:left;padding:9px 13px;font-weight:700}\n.sb-pd td{padding:9px 13px;border-bottom:1px solid #e2eceb;vertical-align:top}\n.sb-pd tr:nth-child(even) td{background:#f8fffe}\n.sb-pd td:first-child{font-weight:600;color:#111;width:35%}\n.sb-pd .qa{background:#f8fffe;border:1px solid #daf3f2;border-radius:8px;padding:14px 16px;margin:10px 0}\n.sb-pd .qa .q{font-weight:700;color:#111;margin:0 0 5px;font-size:14px}\n.sb-pd .qa .a{margin:0;font-size:13px;color:#444}\n.sb-pd .cta{background:linear-gradient(135deg,#e4f6f5,#f4fbfb);border:1px solid rgba(24,183,178,.2);border-radius:12px;padding:20px;margin-top:28px;text-align:center}\n.sb-pd .cta h3{margin:0 0 7px;font-size:17px;color:#111}\n.sb-pd .cta p{margin:0 0 14px;font-size:13px;color:#5b6b71}\n.sb-pd .btn{display:inline-block;background:#18b7b2;color:#fff!important;padding:9px 20px;border-radius:7px;font-weight:700;font-size:13px;text-decoration:none!important;margin:0 4px}\n.sb-pd .btn-o{display:inline-block;border:2px solid #18b7b2;color:#18b7b2!important;padding:7px 20px;border-radius:7px;font-weight:700;font-size:13px;text-decoration:none!important;margin:0 4px}\n.sb-pd a{color:#18b7b2;font-weight:600}\n@media(max-width:600px){.sb-pd .cards{grid-template-columns:1fr}}\n<\/style>\n<div class=\"sb-pd\">\n<h2>Product Overview<\/h2>\n<p><strong>BSA Fatty Acid Free \u2014 Bovine Serum Albumin for CHO, Vero &amp; MDCK Cell Culture<\/strong> is a highly purified, charcoal-delipidated bovine serum albumin with &gt;99% fatty acid removal (&lt;0.01 mol FA\/mol BSA, GC-MS validated), purity \u226598%, and endotoxin &lt;5 EU\/mg (LAL-tested per lot). Sourced from cattle in <strong>Australia, New Zealand, and the USA<\/strong> \u2014 OIE-certified BSE\/TSE-negligible-risk regions \u2014 with full traceability and GC-MS fatty acid analysis per batch.<br \/>\nThe preferred BSA grade wherever exogenous fatty acids would compromise cell line performance, lipid assay accuracy, or regulatory compliance. Browse our full <a href=\"https:\/\/seamlessbio.de\/de\/bsa-frei-von-fettsauren\/\">BSA Fatty Acid Free product page<\/a> or compare grades in our <a href=\"https:\/\/seamlessbio.de\/de\/bsa-albumin-uberblick\/\">BSA &amp; Albumin portfolio<\/a>.<\/p>\n<h2>Why Fatty Acid Free BSA?<\/h2>\n<p>Standard BSA contains 0.5\u20132 mol of naturally bound fatty acids per mol BSA (palmitate, stearate, oleate, linoleate). In most applications these are harmless \u2014 but in three specific scenarios they create measurable problems:<\/p>\n<ul>\n<li><strong>CHO, Vero, MDCK cell culture<\/strong> \u2014 exogenous fatty acids alter glycosylation patterns, disrupt membrane composition, and reduce viral titre; FA-free conditions are preferred by FDA\/EMA for GMP bioproduction<\/li>\n<li><strong>Forschung zum Fettstoffwechsel<\/strong> \u2014 native FA bound to BSA interfere with CD36\/FATP uptake assays, beta-oxidation measurements, and lipoprotein binding studies, creating uncontrolled background signal<\/li>\n<li><strong>Hormone &amp; drug-binding studies<\/strong> \u2014 fatty acids compete for the same albumin binding sites as thyroid hormones (T3\/T4), steroids, and small-molecule drugs, distorting binding affinities and pharmacokinetic measurements<\/li>\n<\/ul>\n<p>Fatty Acid Free BSA is produced by <strong>activated charcoal delipidation<\/strong>, removing &gt;99% of bound fatty acids (confirmed by GC-MS) while maintaining the protein&#8217;s structural integrity and binding capacity. See our <a href=\"https:\/\/seamlessbio.de\/de\/ressourcen\/blog\/bsa-grades-guide-fatty-acid-free-low-endotoxin-standard\/\">BSA Grades Guide<\/a> for a full selection matrix.<\/p>\n<h2>Technische Daten<\/h2>\n<table>\n<thead>\n<tr>\n<th>Parameter<\/th>\n<th>Spezifikation<\/th>\n<th>Test Method<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Quelle<\/td>\n<td>Bovine plasma (AU \/ NZ \/ US)<\/td>\n<td>Certificate of Origin<\/td>\n<\/tr>\n<tr>\n<td>Reinheit<\/td>\n<td>\u226598%<\/td>\n<td>Electrophoresis<\/td>\n<\/tr>\n<tr>\n<td>Albumin content<\/td>\n<td>\u226598 g\/L<\/td>\n<td>Bradford \/ BCA assay<\/td>\n<\/tr>\n<tr>\n<td>Fetts\u00e4uregehalt<\/td>\n<td>&lt;0.01 mol FA\/mol BSA<\/td>\n<td>GC-MS (&gt;99% removal)<\/td>\n<\/tr>\n<tr>\n<td>Endotoxin<\/td>\n<td>&lt;5 EU\/mg<\/td>\n<td>LAL (FDA\/USP standard)<\/td>\n<\/tr>\n<tr>\n<td>Monomers (SEC-HPLC)<\/td>\n<td>\u226595%<\/td>\n<td>Size exclusion chromatography<\/td>\n<\/tr>\n<tr>\n<td>pH<\/td>\n<td>6.8\u20137.5<\/td>\n<td>pH meter<\/td>\n<\/tr>\n<tr>\n<td>Format<\/td>\n<td>Lyophilised powder<\/td>\n<td>\u2014<\/td>\n<\/tr>\n<tr>\n<td>Lagerung<\/td>\n<td>RT sealed; 2\u20138 \u00b0C after opening<\/td>\n<td>\u2014<\/td>\n<\/tr>\n<tr>\n<td>Haltbarkeit<\/td>\n<td>3 years from manufacture<\/td>\n<td>\u2014<\/td>\n<\/tr>\n<tr>\n<td>BSE\/TSE status<\/td>\n<td>Free (OIE-certified regions)<\/td>\n<td>Origin certificate<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>Standard BSA vs. Fatty Acid Free BSA<\/h2>\n<table>\n<thead>\n<tr>\n<th>Eigenschaft<\/th>\n<th>Standard BSA<\/th>\n<th>Fatty Acid Free BSA<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Fetts\u00e4uregehalt<\/td>\n<td>0.5\u20132 mol FA\/mol BSA<\/td>\n<td><strong>&lt;0.01 mol FA\/mol BSA<\/strong><\/td>\n<\/tr>\n<tr>\n<td>Reinheit<\/td>\n<td>\u226596%<\/td>\n<td><strong>\u226598%<\/strong><\/td>\n<\/tr>\n<tr>\n<td>Endotoxin<\/td>\n<td>&lt;10 EU\/mg<\/td>\n<td><strong>&lt;5 EU\/mg<\/strong><\/td>\n<\/tr>\n<tr>\n<td>Ideal for<\/td>\n<td>Western blot, ELISA blocking, protein standard<\/td>\n<td><strong>CHO\/Vero\/MDCK, lipid studies, hormone assays, drug binding<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>Anwendungen<\/h2>\n<ul>\n<li><strong>CHO biomanufacturing (mAbs, recombinant proteins)<\/strong> \u2014 0.5\u20132 g\/L in serum-free media; prevents FA-induced glycosylation disruption; preferred for GMP production per FDA\/EMA guidance<\/li>\n<li><strong>Vero cell vaccine production<\/strong> (Polio, Rabies, Rotavirus, Dengue) \u2014 WHO-certified cell line; FA-free conditions required for regulatory compliance; improved virus titres<\/li>\n<li><strong>MDCK influenza vaccine production<\/strong> \u2014 FA-free BSA increases hemagglutinin (HA) yield; FDA preference for serum-free, FA-free supplements<\/li>\n<li><strong>Lipid transporter &amp; beta-oxidation assays<\/strong> \u2014 CD36, FATP, FABPpm uptake studies; \u00b9\u2074C-palmitate beta-oxidation; lipoprotein metabolism (HDL\/LDL, CETP) \u2014 no background from native FA<\/li>\n<li><strong>Adipocyte differentiation (3T3-L1)<\/strong> \u2014 controlled FA addition without background; clean Oil Red O staining<\/li>\n<li><strong>Hormone &amp; drug-binding studies<\/strong> \u2014 T3\/T4 binding, steroid assays (estrogen, testosterone, cortisol), pharmacokinetic drug-albumin binding \u2014 uncompromised binding sites<\/li>\n<li><strong>Serum-free media formulation<\/strong> \u2014 precise control over lipid composition; targeted addition of defined fatty acids for reproducible, GMP-compliant media<\/li>\n<\/ul>\n<h2>Available Formats<\/h2>\n<table>\n<thead>\n<tr>\n<th>Format<\/th>\n<th>Size<\/th>\n<th>Ideal for<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Gefriergetrocknetes Pulver<\/td>\n<td>10 g<\/td>\n<td>Method development, small labs<\/td>\n<\/tr>\n<tr>\n<td>Gefriergetrocknetes Pulver<\/td>\n<td>50 g<\/td>\n<td>Routine research<\/td>\n<\/tr>\n<tr>\n<td>Gefriergetrocknetes Pulver<\/td>\n<td>100 g<\/td>\n<td>High-throughput labs<\/td>\n<\/tr>\n<tr>\n<td>Gefriergetrocknetes Pulver<\/td>\n<td>500 g<\/td>\n<td>Bioproduction, GMP manufacturing<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Bulk orders \u22651 kg and custom formats available on request \u2014 contact us for a tailored quote.<\/p>\n<h2>Key Benefits<\/h2>\n<div class=\"cards\">\n<div class=\"card\">\n<h3>Sammelreservierung<\/h3>\n<p>Reserve validated lots up to 36 months for reproducible long-term workflows.<\/p>\n<\/div>\n<div class=\"card\">\n<h3>Vollst\u00e4ndige Dokumentation<\/h3>\n<p>CoA, GC-MS fatty acid analysis, LAL endotoxin certificate, BSE\/TSE statement, Certificate of Origin \u2014 ISO 9001 and ISO 13485 certified partner facilities.<\/p>\n<\/div>\n<div class=\"card\">\n<h3>EU &amp; Swiss Supply<\/h3>\n<p>Sourced from certified EU and Swiss partner facilities \u2014 fast delivery across EU, UK and Switzerland, full documentation included.<\/p>\n<\/div>\n<\/div>\n<h2>H\u00e4ufig gestellte Fragen<\/h2>\n<div class=\"qa\">\n<p class=\"q\">Why do CHO cells specifically require Fatty Acid Free BSA rather than standard BSA?<\/p>\n<p class=\"a\">CHO cells are highly sensitive to exogenous fatty acids: native FA bound to standard BSA are released into the culture medium, where they integrate into cell membranes and alter N-glycosylation patterns of secreted proteins \u2014 a critical quality attribute for monoclonal antibodies and recombinant biopharmaceuticals. FA-free BSA eliminates this variable, giving tighter control over product quality. FDA and EMA guidance for GMP mAb production increasingly favours FA-free or chemically defined media supplements for this reason.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">What method is used to remove fatty acids, and how is removal validated?<\/p>\n<p class=\"a\">Fatty acids are removed by activated charcoal delipidation \u2014 the BSA solution is incubated with activated charcoal, which adsorbs the hydrophobic FA from the protein. After delipidation, residual FA content is measured by gas chromatography\u2013mass spectrometry (GC-MS). Each lot is certified at &lt;0.01 mol FA\/mol BSA, corresponding to &gt;99% FA removal. The GC-MS analysis report is included with every batch.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">Can Fatty Acid Free BSA be used to deliver specific fatty acids to cells?<\/p>\n<p class=\"a\">Yes \u2014 this is one of its primary uses. Because FA-free BSA has unoccupied fatty acid binding sites, it serves as an ideal carrier for delivering defined, exogenously added fatty acids (e.g., oleic acid, palmitate, linoleic acid) to cells in a controlled and reproducible manner. This is the standard approach in adipocyte differentiation, beta-oxidation assays, and serum-free media formulation where precise lipid control is required.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">Is BSA Fatty Acid Free suitable for GMP vaccine production in Vero or MDCK cells?<\/p>\n<p class=\"a\">Yes \u2014 Fatty Acid Free BSA is the preferred BSA grade for Vero-based (Polio, Rabies, Rotavirus, Dengue) and MDCK-based (influenza) vaccine production. The WHO-certified Vero cell line and influenza vaccine processes developed for FDA submission both recommend FA-free supplements in serum-free media. FA-free conditions have been associated with improved viral titres and higher hemagglutinin (HA) yield in MDCK influenza processes. Full regulatory documentation (CoA, GC-MS, BSE\/TSE, Certificate of Origin) is available for GMP raw material qualification.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">What documentation is supplied, and is batch reservation available?<\/p>\n<p class=\"a\">Every order includes: Certificate of Analysis (CoA), GC-MS fatty acid analysis (&lt;0.01 mol FA\/mol BSA confirmed), LAL endotoxin test certificate (&lt;5 EU\/mg), BSE\/TSE freedom declaration, and Certificate of Origin (AU\/NZ\/US). Batch reservation is available for up to 36 months \u2014 contact our team with your volume and timeline. Bulk orders \u22651 kg are available with custom pricing. There is no fixed minimum order quantity.<\/p>\n<\/div>\n<div class=\"cta\">\n<h3>Request a Quote or Sample<\/h3>\n<p>48h response \u00b7 EU &amp; Swiss Supply \u00b7 Batch reservation available \u00b7 GC-MS validated per lot<\/p>\n<p>  <a href=\"https:\/\/seamlessbio.de\/de\/request-quote\/\" class=\"btn\">Request Quote \u2192<\/a><br \/>\n  <a href=\"https:\/\/seamlessbio.de\/de\/bsa-albumin-uberblick\/\" class=\"btn-o\">BSA &amp; Albumin Portfolio \u2192<\/a>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>BSA Fetts\u00e4urefrei <0.01% | \u226599% Purity | US Origin | Powder. Available in 50g, 100g, 500g. Price on request \u2014 contact our team for availability, batch documentation and delivery. \n\n<div style='margin-top:12px'><a href='https:\/\/seamlessbio.de\/de\/sammelreservierung\/' style='display:block;background:#fff;border:2px solid #18b7b2;color:#18b7b2;padding:10px 24px;border-radius:8px;font-weight:700;font-size:14px;text-decoration:none;text-align:center;'>Test Sample &amp; Batch Reservation \u2192<\/a><\/div>","protected":false},"featured_media":3649,"template":"","meta":{"_eb_attr":""},"product_brand":[],"product_cat":[232],"product_tag":[233,238,239,240,241],"class_list":["post-3598","product","type-product","status-publish","has-post-thumbnail","product_cat-bsa","product_tag-bovine-serum-albumin","product_tag-bsa-powder","product_tag-fatty-acid-free-bsa","product_tag-high-purity-bovine-serum-albumin","product_tag-us-origin-bsa","first","instock","taxable","shipping-taxable","product-type-simple"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 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