{"id":3600,"date":"2026-09-16T12:10:26","date_gmt":"2026-09-16T11:10:26","guid":{"rendered":"https:\/\/wheat-quail-856400.hostingersite.com\/produkt\/simplacex-serum-free-medium-for-hek293t\/"},"modified":"2026-10-26T12:57:59","modified_gmt":"2026-10-26T11:57:59","slug":"simplacex-serumfreies-medium-hek293t","status":"publish","type":"product","link":"https:\/\/seamlessbio.de\/de\/product\/simplacex-serum-free-medium-for-hek293t\/","title":{"rendered":"SimplaceX \u2013 serumfreies Medium f\u00fcr HEK293T"},"content":{"rendered":"<style>\n.sb-pd{font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",Roboto,sans-serif;max-width:100%;color:#202020;line-height:1.75}\n.sb-pd .def{background:#e9fbfa;border-left:4px solid #18b7b2;border-radius:0 10px 10px 0;padding:16px 20px;margin:0 0 28px}\n.sb-pd .def .lb{font-size:11px;font-weight:800;text-transform:uppercase;letter-spacing:.09em;color:#139b97;display:block;margin-bottom:5px}\n.sb-pd .def p{font-size:15px;margin:0;font-weight:500}\n.sb-pd h2{font-size:21px;font-weight:800;margin:32px 0 10px;color:#111;border-bottom:2px solid #e2eceb;padding-bottom:7px}\n.sb-pd h3{font-size:15px;font-weight:700;color:#18b7b2;margin:18px 0 5px}\n.sb-pd p{margin:0 0 12px;font-size:15px}\n.sb-pd ul{margin:0 0 12px;padding-left:20px}\n.sb-pd li{margin-bottom:6px;font-size:14px}\n.sb-pd .cards{display:grid;grid-template-columns:repeat(3,1fr);gap:12px;margin:14px 0 20px}\n.sb-pd .card{background:#f1faf9;border:1px solid #daf3f2;border-top:3px solid #18b7b2;border-radius:0 0 10px 10px;padding:14px}\n.sb-pd .card h3{margin:0 0 5px;font-size:13px;color:#111}\n.sb-pd .card p{margin:0;font-size:12px;color:#5b6b71}\n.sb-pd table{width:100%;border-collapse:collapse;font-size:14px;margin:14px 0 20px}\n.sb-pd th{background:#18b7b2;color:#fff;text-align:left;padding:9px 13px;font-weight:700}\n.sb-pd td{padding:9px 13px;border-bottom:1px solid #e2eceb;vertical-align:top}\n.sb-pd tr:nth-child(even) td{background:#f8fffe}\n.sb-pd td:first-child{font-weight:600;color:#111;width:35%}\n.sb-pd .qa{background:#f8fffe;border:1px solid #daf3f2;border-radius:8px;padding:14px 16px;margin:10px 0}\n.sb-pd .qa .q{font-weight:700;color:#111;margin:0 0 5px;font-size:14px}\n.sb-pd .qa .a{margin:0;font-size:13px;color:#444}\n.sb-pd .cta{background:linear-gradient(135deg,#e4f6f5,#f4fbfb);border:1px solid rgba(24,183,178,.2);border-radius:12px;padding:20px;margin-top:28px;text-align:center}\n.sb-pd .cta h3{margin:0 0 7px;font-size:17px;color:#111}\n.sb-pd .cta p{margin:0 0 14px;font-size:13px;color:#5b6b71}\n.sb-pd .btn{display:inline-block;background:#18b7b2;color:#fff!important;padding:9px 20px;border-radius:7px;font-weight:700;font-size:13px;text-decoration:none!important;margin:0 4px}\n.sb-pd .btn-o{display:inline-block;border:2px solid #18b7b2;color:#18b7b2!important;padding:7px 20px;border-radius:7px;font-weight:700;font-size:13px;text-decoration:none!important;margin:0 4px}\n.sb-pd a{color:#18b7b2;font-weight:600}\n@media(max-width:600px){.sb-pd .cards{grid-template-columns:1fr}}\n<\/style>\n<div class=\"sb-pd\">\n<h2>Produkt\u00fcbersicht<\/h2>\n<p><strong>SimplaceX \u2013 serumfreies Medium f\u00fcr HEK293T<\/strong> ist ein proteinsupplementiertes, serumfreies Zellkulturmedium, optimiert f\u00fcr ertragreiche transiente Transfektion und stabile Expression in <strong>HEK293- und HEK293T-Zelllinien<\/strong>.<br \/>\nEntwickelt f\u00fcr die schnelle Adaptation aus serumhaltigen Protokollen, unterst\u00fctzt SimplaceX hohe Zelldichten, sehr gute Viabilit\u00e4t und robuste Ausbeuten an rekombinanten Proteinen \u2013 ohne die Chargenschwankungen von FBS. Erh\u00e4ltlich bei SeamlessBio mit vollst\u00e4ndiger Dokumentation und Chargenreservierung. Mehr \u00fcber unser <a href=\"https:\/\/seamlessbio.de\/de\/zellkultur\/\">Zellkulturmedien-Sortiment<\/a> oder auf unserer <a href=\"https:\/\/seamlessbio.de\/de\/simplacex-serumfreies-hek293t-medium\/\">SimplaceX-Produktseite<\/a>.<\/p>\n<h2>SimplaceX: Spezifikationen<\/h2>\n<ul>\n<li>Typ: serumfreies, proteinsupplementiertes Medium (nicht chemisch definiert)<\/li>\n<li>Zelllinien: HEK293, HEK293T \u2013 optimiert f\u00fcr transiente und stabile Expression<\/li>\n<li>Adaptation: schrittweise FBS-Reduktion; vollst\u00e4ndige Adaptation in 5\u201310 Passagen<\/li>\n<li>Sterilit\u00e4t: sterilfiltriert, auf Mykoplasmen gepr\u00fcft<\/li>\n<li>Lagerung: 2\u20138 \u00b0C (lichtgesch\u00fctzt)<\/li>\n<li>Dokumentation: vollst\u00e4ndiges technisches Datenblatt, CoA, Sterilit\u00e4tszertifikat inklusive<\/li>\n<\/ul>\n<h2>Anwendungen<\/h2>\n<ul>\n<li>Transiente Transfektion f\u00fcr rekombinante Proteine und Antik\u00f6rper (HEK293T)<\/li>\n<li>Herstellung viraler Vektoren (Lentivirus, AAV) in HEK293-basierten Systemen<\/li>\n<li>Entwicklung und Selektion stabiler Zelllinien<\/li>\n<li>Scale-up vom Sch\u00fcttelkolben in den Bioreaktor unter serumfreien Bedingungen<\/li>\n<li>Prozessentwicklung mit dem Ziel, FBS zu entfernen \u2013 siehe unser komplettes <a href=\"https:\/\/seamlessbio.de\/de\/zellkultur\/\">Zellkultur-Sortiment<\/a><\/li>\n<\/ul>\n<h2>Verf\u00fcgbare Formate<\/h2>\n<table>\n<thead>\n<tr>\n<th>Format<\/th>\n<th>Gr\u00f6\u00dfe<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Flasche<\/td>\n<td>500 mL<\/td>\n<\/tr>\n<tr>\n<td>Flasche<\/td>\n<td>2000 mL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>Die wichtigsten Vorteile<\/h2>\n<div class=\"cards\">\n<div class=\"card\">\n<h3>Sammelreservierung<\/h3>\n<p>Validierte Chargen bis zu 36 Monate reservieren \u2013 f\u00fcr reproduzierbare Langzeit-Workflows.<\/p>\n<\/div>\n<div class=\"card\">\n<h3>Vollst\u00e4ndige Dokumentation<\/h3>\n<p>CoA, BSE\/TSE, Sterilit\u00e4tszertifikat \u2013 von nach ISO 9001 und ISO 13485 zertifizierten Partnerwerken. Weitere Dokumente auf Anfrage.<\/p>\n<\/div>\n<div class=\"card\">\n<h3>Lieferung EU &amp; Schweiz<\/h3>\n<p>Bezogen aus zertifizierten Partnerwerken in der EU und der Schweiz \u2013 schnelle Lieferung in EU, UK und Schweiz, vollst\u00e4ndige Dokumentation inklusive.<\/p>\n<\/div>\n<\/div>\n<h2>H\u00e4ufig gestellte Fragen<\/h2>\n<div class=\"qa\">\n<p class=\"q\">Was ist SimplaceX und wie unterscheidet es sich von serumhaltigem Standard-HEK293-Medium?<\/p>\n<p class=\"a\">SimplaceX ist ein serumfreies, proteinsupplementiertes Medium speziell f\u00fcr HEK293- und HEK293T-Zellen. Anders als Standard-DMEM + 10 % FBS kommt SimplaceX ohne tierisches Serum aus und erh\u00e4lt dennoch hohe Viabilit\u00e4t und Transfektionseffizienz. Es ist proteinsupplementiert (nicht vollst\u00e4ndig chemisch definiert) und damit eine praktische Br\u00fccke zwischen serumbasierten und vollst\u00e4ndig definierten CDM-Prozessen.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">Wie viele Passagen dauert die Adaptation von HEK293T an SimplaceX?<\/p>\n<p class=\"a\">Die vollst\u00e4ndige Adaptation dauert typischerweise 5\u201310 Passagen mit schrittweiser FBS-Reduktion: Senken Sie die FBS-Konzentration pro Passage um 2\u20133 % und erg\u00e4nzen Sie SimplaceX. Die meisten HEK293T-Linien erreichen in 100 % SimplaceX innerhalb von 7\u20138 Passagen &gt;95 % Viabilit\u00e4t. Ein ausf\u00fchrliches Adaptationsprotokoll liegt dem technischen Datenblatt bei.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">Was ist der Unterschied zwischen SimplaceX und SimplaceCDX?<\/p>\n<p class=\"a\">SimplaceX ist serumfrei, enth\u00e4lt aber Proteinsupplemente (z. B. rekombinantes Albumin oder Transferrin). SimplaceCDX ist vollst\u00e4ndig chemisch definiert \u2013 jede Komponente ist bekannt und spezifiziert, ohne undefinierte Proteinfraktionen. SimplaceCDX ist f\u00fcr GMP-Prozesse n\u00f6tig, in denen die regulatorische Dokumentation ein vollst\u00e4ndiges CDM (chemisch definiertes Medium) verlangt. SimplaceX ist der bevorzugte Einstieg f\u00fcr die meisten Forschungs- und Prozessentwicklungs-Workflows auf dem Weg weg von FBS.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">Ist SimplaceX f\u00fcr die Produktion von AAV oder lentiviralen Vektoren geeignet?<\/p>\n<p class=\"a\">Ja \u2013 SimplaceX unterst\u00fctzt die HEK293T-basierte Vektorproduktion, einschlie\u00dflich transienter Tripel- oder Quadrupeltransfektion f\u00fcr Lentivirus und AAV. Serumfreie Bedingungen vereinfachen die Aufreinigung, weil keine Serumproteine in der Ernte sind. F\u00fcr GMP-Vektoranwendungen mit vollst\u00e4ndiger CDM-Dokumentation empfehlen wir SimplaceCDX.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">L\u00e4sst sich SimplaceX in Bioreaktoren oder nur in statischer Kultur einsetzen?<\/p>\n<p class=\"a\">SimplaceX ist mit statischer Kultur (Flaschen, Multiwell-Platten) und dynamischen Bioreaktorsystemen (R\u00fchrkessel, Rocking-Bioreaktoren) kompatibel. Im Bioreaktor kann je nach System ein Antischaummittel n\u00f6tig sein. Kontaktieren Sie unser Team f\u00fcr anwendungsspezifische Beratung und ein Muster zur Prozessevaluierung.<\/p>\n<\/div>\n<div class=\"cta\">\n<h3>Angebot oder Muster anfordern<\/h3>\n<p>Antwort in 48 h \u00b7 Lieferung EU &amp; Schweiz \u00b7 Chargenreservierung m\u00f6glich \u00b7 Vollst\u00e4ndige Dokumentation<\/p>\n<p>  <a href=\"https:\/\/seamlessbio.de\/de\/request-quote\/\" class=\"btn\">Angebot anfordern \u2192<\/a><br \/>\n  <a href=\"https:\/\/seamlessbio.de\/de\/zellkultur\/\" class=\"btn-o\">Zellkultur-Sortiment \u2192<\/a>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>SimplaceX \u2013 serumfreies Medium f\u00fcr HEK293T. Erh\u00e4ltlich in 500 ml | 2.000 ml. Preis auf Anfrage \u2013 kontaktieren Sie unser Team zu Verf\u00fcgbarkeit und Dokumentation. <\/p>\n<div style='margin-top:12px'><a href='https:\/\/seamlessbio.de\/de\/chargenreservierung\/' style='display:block;background:#fff;border:2px solid #18b7b2;color:#18b7b2;padding:10px 24px;border-radius:8px;font-weight:700;font-size:14px;text-decoration:none;text-align:center;'>Test Sample &amp; Batch Reservation \u2192<\/a><\/div>","protected":false},"featured_media":0,"template":"","meta":{"_templately_pack_id":"","_templately_imported_at":"","_templately_source":"","_templately_import_session_id":""},"product_brand":[],"product_cat":[244],"product_tag":[],"class_list":["post-3600","product","type-product","status-publish","product_cat-cell-culture-media","first","instock","taxable","shipping-taxable","product-type-simple"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Buy SimplaceX Serum-Free HEK293T Medium | EU Supplier | SeamlessBio<\/title>\n<meta name=\"description\" content=\"SimplaceX serum-free medium for HEK293T suspension culture and AAV production, 500 mL and 2,000 mL. EU stock, free test sample, no minimum order \u2013 request a quote.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/seamlessbio.de\/de\/produkt\/simplacex-serumfreies-medium-hek293t\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Buy SimplaceX Serum-Free HEK293T Medium | EU Supplier | SeamlessBio\" \/>\n<meta property=\"og:description\" content=\"SimplaceX serum-free medium for HEK293T suspension culture and AAV production, 500 mL and 2,000 mL. 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