{"id":3601,"date":"2026-09-16T12:10:26","date_gmt":"2026-09-16T11:10:26","guid":{"rendered":"https:\/\/seamlessbio.de\/produkt\/simplacecdx-chemically-defined-medium-for-hek293t\/"},"modified":"2026-09-19T16:51:32","modified_gmt":"2026-09-19T15:51:32","slug":"simplacecdx-chemically-defined-medium-for-hek293t","status":"publish","type":"product","link":"https:\/\/seamlessbio.de\/de\/product\/simplacecdx-chemically-defined-medium-for-hek293t\/","title":{"rendered":"SimplaceCDX \u2014 Chemically Defined Medium for HEK293T"},"content":{"rendered":"<style>\n.sb-pd{font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",Roboto,sans-serif;max-width:100%;color:#202020;line-height:1.75}\n.sb-pd .def{background:#e9fbfa;border-left:4px solid #18b7b2;border-radius:0 10px 10px 0;padding:16px 20px;margin:0 0 28px}\n.sb-pd .def .lb{font-size:11px;font-weight:800;text-transform:uppercase;letter-spacing:.09em;color:#139b97;display:block;margin-bottom:5px}\n.sb-pd .def p{font-size:15px;margin:0;font-weight:500}\n.sb-pd h2{font-size:21px;font-weight:800;margin:32px 0 10px;color:#111;border-bottom:2px solid #e2eceb;padding-bottom:7px}\n.sb-pd h3{font-size:15px;font-weight:700;color:#18b7b2;margin:18px 0 5px}\n.sb-pd p{margin:0 0 12px;font-size:15px}\n.sb-pd ul{margin:0 0 12px;padding-left:20px}\n.sb-pd li{margin-bottom:6px;font-size:14px}\n.sb-pd .cards{display:grid;grid-template-columns:repeat(3,1fr);gap:12px;margin:14px 0 20px}\n.sb-pd .card{background:#f1faf9;border:1px solid #daf3f2;border-top:3px solid #18b7b2;border-radius:0 0 10px 10px;padding:14px}\n.sb-pd .card h3{margin:0 0 5px;font-size:13px;color:#111}\n.sb-pd .card p{margin:0;font-size:12px;color:#5b6b71}\n.sb-pd table{width:100%;border-collapse:collapse;font-size:14px;margin:14px 0 20px}\n.sb-pd th{background:#18b7b2;color:#fff;text-align:left;padding:9px 13px;font-weight:700}\n.sb-pd td{padding:9px 13px;border-bottom:1px solid #e2eceb;vertical-align:top}\n.sb-pd tr:nth-child(even) td{background:#f8fffe}\n.sb-pd td:first-child{font-weight:600;color:#111;width:35%}\n.sb-pd .qa{background:#f8fffe;border:1px solid #daf3f2;border-radius:8px;padding:14px 16px;margin:10px 0}\n.sb-pd .qa .q{font-weight:700;color:#111;margin:0 0 5px;font-size:14px}\n.sb-pd .qa .a{margin:0;font-size:13px;color:#444}\n.sb-pd .cta{background:linear-gradient(135deg,#e4f6f5,#f4fbfb);border:1px solid rgba(24,183,178,.2);border-radius:12px;padding:20px;margin-top:28px;text-align:center}\n.sb-pd .cta h3{margin:0 0 7px;font-size:17px;color:#111}\n.sb-pd .cta p{margin:0 0 14px;font-size:13px;color:#5b6b71}\n.sb-pd .btn{display:inline-block;background:#18b7b2;color:#fff!important;padding:9px 20px;border-radius:7px;font-weight:700;font-size:13px;text-decoration:none!important;margin:0 4px}\n.sb-pd .btn-o{display:inline-block;border:2px solid #18b7b2;color:#18b7b2!important;padding:7px 20px;border-radius:7px;font-weight:700;font-size:13px;text-decoration:none!important;margin:0 4px}\n.sb-pd a{color:#18b7b2;font-weight:600}\n@media(max-width:600px){.sb-pd .cards{grid-template-columns:1fr}}\n<\/style>\n<div class=\"sb-pd\">\n<h2>Product Overview<\/h2>\n<p><strong>SimplaceCDX \u2013 Chemically Defined Medium for HEK293T<\/strong> is a fully chemically defined, animal-origin-free cell culture medium engineered for high-yield transient transfection, stable expression, and viral vector manufacturing in <strong>HEK293 and HEK293T cell lines<\/strong>.<br \/>\nEvery component is precisely specified \u2014 no undefined protein fractions, no animal-derived ingredients \u2014 making SimplaceCDX the medium of choice for GMP bioprocessing where a complete CDM (chemically defined medium) is required in regulatory documentation (IND, CTA, BLA). Available from SeamlessBio with full CDM documentation, batch reservation, and EU &amp; Swiss supply. Explore our full <a href=\"https:\/\/seamlessbio.de\/de\/zellkultur\/\">cell culture media portfolio<\/a> or visit the <a href=\"https:\/\/seamlessbio.de\/de\/simplacecdx-hek293-chemically-defined-medium\/\">SimplaceCDX product page<\/a>.<\/p>\n<h2>SimplaceCDX: Specifications<\/h2>\n<ul>\n<li>Type: Fully chemically defined medium (CDM) \u2014 animal-origin-free, no undefined protein fractions<\/li>\n<li>Cell lines: HEK293, HEK293T \u2014 optimised for transient and stable expression<\/li>\n<li>Adaptation: Recommended via <a href=\"https:\/\/seamlessbio.de\/de\/simplacex-hek293-serum-free-medium\/\">SimplaceX<\/a> (serum-free bridge): FBS \u2192 SimplaceX (5\u201310 passages) \u2192 SimplaceCDX (4 passages)<\/li>\n<li>Sterility: Sterile filtered, mycoplasma tested<\/li>\n<li>Storage: 2\u20138 \u00b0C (protect from light)<\/li>\n<li>Documentation: Full CDM documentation, CoA, sterility certificate \u2014 suitable for IND\/CTA CMC sections<\/li>\n<\/ul>\n<h2>Anwendungen<\/h2>\n<ul>\n<li>GMP viral vector manufacturing \u2014 AAV and lentivirus production in HEK293T-based systems<\/li>\n<li>Transient transfection for recombinant protein and antibody production requiring CDM documentation<\/li>\n<li>ATMP and gene therapy process development where animal-origin-free media are mandated<\/li>\n<li>Stable cell line development and selection under fully defined conditions<\/li>\n<li>Regulatory submissions (IND, CTA, BLA) requiring complete characterisation of all medium components \u2014 see our full <a href=\"https:\/\/seamlessbio.de\/de\/zellkultur\/\">cell culture portfolio<\/a><\/li>\n<\/ul>\n<h2>Available Formats<\/h2>\n<table>\n<thead>\n<tr>\n<th>Format<\/th>\n<th>Size<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Bottle<\/td>\n<td>500 mL<\/td>\n<\/tr>\n<tr>\n<td>Bottle<\/td>\n<td>2000 mL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>Key Benefits<\/h2>\n<div class=\"cards\">\n<div class=\"card\">\n<h3>Sammelreservierung<\/h3>\n<p>Reserve validated lots up to 36 months for reproducible long-term workflows.<\/p>\n<\/div>\n<div class=\"card\">\n<h3>Vollst\u00e4ndige Dokumentation<\/h3>\n<p>CoA, BSE\/TSE, sterility certificate \u2014 supplied by ISO 9001 and ISO 13485 certified partner facilities. Additional documents on request.<\/p>\n<\/div>\n<div class=\"card\">\n<h3>EU &amp; Swiss Supply<\/h3>\n<p>Sourced from certified EU and Swiss partner facilities \u2014 fast delivery across EU, UK and Switzerland, full documentation included.<\/p>\n<\/div>\n<\/div>\n<h2>H\u00e4ufig gestellte Fragen<\/h2>\n<div class=\"qa\">\n<p class=\"q\">What does &#8220;chemically defined&#8221; mean and why does it matter for GMP manufacturing?<\/p>\n<p class=\"a\">A chemically defined medium (CDM) contains only components whose identity, purity, and concentration are precisely known and specified. Unlike serum-containing or protein-supplemented media, a CDM has no undefined animal-derived fractions. This is essential for GMP manufacturing: regulatory agencies (EMA, FDA) require full characterisation of all raw materials used in ATMP and viral vector production. SimplaceCDX provides the CDM documentation required for IND, CTA, and BLA submissions.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">Is SimplaceCDX suitable for GMP AAV manufacturing in HEK293T cells?<\/p>\n<p class=\"a\">Yes \u2014 SimplaceCDX is fully chemically defined and animal-origin-free, making it directly applicable to GMP AAV manufacturing in HEK293T-based triple or quadruple transfection systems. Its CDM documentation supports CMC sections in IND and CTA filings. For research-scale or process development work not requiring full CDM documentation, <a href=\"https:\/\/seamlessbio.de\/de\/simplacex-hek293-serum-free-medium\/\">SimplaceX<\/a> is the recommended starting point.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">Can I adapt HEK293T cells directly from FBS to SimplaceCDX?<\/p>\n<p class=\"a\">Direct adaptation from FBS to SimplaceCDX is not recommended, as the large compositional change typically results in reduced viability and extended recovery. The validated pathway is: FBS \u2192 <a href=\"https:\/\/seamlessbio.de\/de\/simplacex-hek293-serum-free-medium\/\">SimplaceX<\/a> (5\u201310 passages, stepwise FBS reduction) \u2192 SimplaceCDX (4 passages). This two-step protocol consistently achieves &gt;95% viability in 100% SimplaceCDX within 14 passages total.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">What documentation is supplied with SimplaceCDX?<\/p>\n<p class=\"a\">Every order includes a Certificate of Analysis (CoA), sterility certificate, and mycoplasma test results. Full CDM documentation \u2014 including component specifications suitable for IND\/CTA CMC sections \u2014 is available on request. BSE\/TSE statements and additional regulatory documents can be provided for GMP customers. Contact our team to discuss your specific documentation requirements before ordering.<\/p>\n<\/div>\n<div class=\"qa\">\n<p class=\"q\">What is the difference between SimplaceCDX and SimplaceX?<\/p>\n<p class=\"a\">SimplaceX is serum-free but protein-supplemented \u2014 it contains defined protein fractions (e.g. recombinant albumin or transferrin) that are not fully characterised at the component level. SimplaceCDX is fully chemically defined: every component is known, specified, and documented. SimplaceX is the preferred choice for research and process development; SimplaceCDX is required wherever regulatory documentation mandates a fully defined, animal-origin-free medium.<\/p>\n<\/div>\n<div class=\"cta\">\n<h3>Request a Quote or Sample<\/h3>\n<p>48h response \u00b7 EU &amp; Swiss Supply \u00b7 Batch reservation available \u00b7 CDM documentation included<\/p>\n<p>  <a href=\"https:\/\/seamlessbio.de\/de\/request-quote\/\" class=\"btn\">Request Quote \u2192<\/a><br \/>\n  <a href=\"https:\/\/seamlessbio.de\/de\/zellkultur\/\" class=\"btn-o\">Cell Culture Portfolio \u2192<\/a>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>SimplaceCDX \u2014 Chemically Defined Medium for HEK293T. Available in 500mL | 2000mL. Price on request \u2014 contact our team for availability and documentation. <\/p>\n<div style='margin-top:12px'><a href='https:\/\/seamlessbio.de\/de\/sammelreservierung\/' style='display:block;background:#fff;border:2px solid #18b7b2;color:#18b7b2;padding:10px 24px;border-radius:8px;font-weight:700;font-size:14px;text-decoration:none;text-align:center;'>Test Sample &amp; Batch Reservation \u2192<\/a><\/div>","protected":false},"featured_media":0,"template":"","meta":{"_eb_attr":""},"product_brand":[],"product_cat":[244],"product_tag":[],"class_list":["post-3601","product","type-product","status-publish","product_cat-cell-culture-media","first","instock","taxable","shipping-taxable","product-type-simple"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>SimplaceCDX \u2014 Chemically Defined Medium for HEK293T | SeamlessBio<\/title>\n<meta name=\"description\" content=\"SimplaceCDX \u2014 Chemically Defined Medium for HEK293T \u2014 available from SeamlessBio. 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