DMPK- und Transporter-Bausätze
ABC transporter vesicle kits and drug transporter assay services for IND-enabling DMPK packages. HEK293-derived inside-out membrane vesicles for BSEP, BCRP, P-gp and MRP transporter inhibition and substrate identification studies — aligned with FDA 2020, EMA 2012 and ICH M12 requirements.
Complete ABC transporter panel — 10 kits available
Products & Services
ABC Transporter Vesicle Kits
Cell4Pharma · HEK293-derivedReady-to-use inside-out membrane vesicles for quantitative ABC transporter inhibition and substrate identification. All kits include vesicles, probe substrate, buffers, positive inhibitor control, and AMP negative control.
View All Vesicle Kits →Drug Transporter Assay Service
Ciptec · CRO Assay ServiceFull-service DMPK transporter assay outsourcing via Ciptec. Submit your compound — receive IC50 values, inhibition data, and regulatory-ready reports for ABC transporter panels without in-house infrastructure.
- Complete panel or individual transporter
- IC50 determination and inhibition %
- GLP-compliant study design available
- Regulatory-ready reports (FDA/EMA format)
- Fast turnaround — typically 2–4 weeks
Regulatory Requirements at a Glance
ABC transporter assessment is mandated for all new chemical entities (NCEs) before first-in-human dosing. The following table summarises which transporters are required under major regulatory frameworks.
| Transporter | Gene | FDA 2020 | EMA 2012 | ICH M12 | Primary Use Case |
|---|---|---|---|---|---|
| BSEP | ABCB11 | ⚠️ Recommended | ⚠️ Recommended | ⚠️ Recommended | DILI / cholestatic hepatotoxicity risk |
| P-gp | ABCB1 | ✅ Required | ✅ Required | ✅ Required | Intestinal / BBB efflux, bioavailability |
| BCRP | ABCG2 | ✅ Required | ✅ Required | ✅ Required | Intestinal / hepatic / renal efflux |
| MRP2 | ABCC2 | Risk-based | ✅ Recommended | Risk-based | Hepatobiliary efflux of conjugates |
| MRP4 | ABCC4 | Recommended | Risk-based | Recommended | Renal tubular secretion, nucleotide analogues |
| MRP1,3,5,8 | ABCC1/3/5/11 | Risk-based | Risk-based | Risk-based | MDR, glucuronide efflux, nucleoside analogues |
Complete Kit Specifications
| Kit | Gene | Probe Substrate | Key Application | Order |
|---|---|---|---|---|
| BSEP-Vesikel-Kit | ABCB11 | Taurocholic acid | DILI / cholestasis risk assessment | Order → |
| BCRP-Vesikel-Kit | ABCG2 | Estrone-3-sulphate | FDA/EMA mandatory — efflux inhibition | Order → |
| P-gp-Vesikel-Kit | ABCB1 | N-methyl quinidine | FDA/EMA mandatory — efflux inhibition | Order → |
| MRP1-Vesikel-Kit | ABCC1 | LTC4 / E217βG | MDR / oncology programs | Order → |
| MRP2-Vesikel-Kit | ABCC2 | E217βG | Hepatobiliary disposition | Order → |
| MRP3 Vesicle Kit | ABCC3 | E217βG | Glucuronide conjugate efflux | Order → |
| MRP4 Vesicle Kit | ABCC4 | DHEAS | Renal tubular secretion | Order → |
| MRP5 Vesicle Kit | ABCC5 | cGMP | Nucleoside analogue resistance | Order → |
| MRP8 Vesicle Kit | ABCC11 | cAMP | Nucleoside analogue programs | Order → |
| Control Vesicles | — | — | Background correction (required) | Order → |
All kits: 100 reactions · HEK293-derived · Guaranteed transport ratio per lot · All buffers included · Lot-specific CoA · Shipped on dry ice at −80°C · No minimum order quantity
Why Cell4Pharma Vesicle Kits via SeamlessBio
Human Expression System
HEK293-derived vesicles provide human-like protein folding, glycosylation and membrane lipid environment — closer to native hepatic/intestinal transporter conformation than Sf9/baculovirus systems.
Guaranteed Transport Ratio
Every lot is characterised and ships with a guaranteed transport ratio (ATP vs AMP uptake) — ensuring assay performance before you run your compound.
Complete Panel Available
10 transporters including rare variants (MRP5, MRP8) often unavailable from larger suppliers. Control vesicles matched to every transporter kit.
EU Supply Chain
Distributed via SeamlessBio from the EU — shorter lead times, no transatlantic customs delays, euro-denominated pricing. Batch reservation available.
Related Application Guides
Order Kits or Request a Panel Quote
Individual kits available in the shop. Full DMPK panel pricing, bulk orders and assay service quotes on request.
info@seamlessbio.de · +49 851 37932226
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