NaivaCore
Defined three-component medium for naive reprogramming, expansion and long-term maintenance of human pluripotent stem cells.
Three-component architecture — separate vials for protocol flexibility
Three-Component System
NaivaCore is supplied as three separate components, each optimised for independent storage and flexible use. This architecture allows concentration adjustments, independent lot qualification, and stable long-term storage of thermolabile inhibitors.
NaivaCore-B
Basal Medium — N2B27 BaseDefined, serum-free N2B27-based basal medium. Combines Neurobasal (50%) and DMEM/F-12 (50%) with essential supplements for naive PSC support.
| Component | Function |
|---|---|
| Neurobasal / DMEM/F-12 (1:1) | Base nutrients |
| Penicillin/Streptomycin | Contamination control |
| Sodium Pyruvate | Energy metabolism |
| NEAA | Amino acid supplementation |
| GlutaMAX | Stable glutamine source |
| L-Ascorbic Acid-2-Phosphate | Antioxidant, collagen support |
NaivaCore-SI ×100
Small Molecule Inhibitor Supplement100× concentrate of eight small molecule inhibitors and two cytokines targeting the signalling pathways required for naive ground state induction and maintenance.
| Inhibitor | Target | Conc. (1×) |
|---|---|---|
| LIF | JAK/STAT3 | 20 ng/mL |
| Activin A | Nodal/SMAD2 | 5 ng/mL |
| XAV939 | WNT/Tankyrase | 2 µM |
| Go6983 | PKC | 2 µM |
| PD0325901 | MEK/ERK | 1.2 µM |
| CGP77675 | SRC kinase | 1.2 µM |
| Y-27632 | ROCK | 1.2 µM |
| BIRB 796 | p38/JNK | 0.8 µM |
| Geltrex Flex | Matrix support | 0.2% |
NaivaCore-NT ×50
Neurotrophic & Antioxidant Supplement50× BSA-based concentrate combining neurotrophic factors, antioxidant enzymes, vitamins, and lipid supplements — derived from the N2/B27 supplement tradition, optimised for naive PSC metabolic requirements.
| Component | Class |
|---|---|
| rhInsulin, Holo-Transferrin | Growth factors |
| Catalase, SOD, Glutathione | Antioxidant enzymes |
| Sodium Selenite | Trace element / redox |
| Putrescine, Progesterone | Neurotrophic factors |
| T3, Corticosterone | Hormones |
| L-Carnitine, Ethanolamine | Metabolic support |
| Linoleic/Linolenic acid, Oleic acid | Essential fatty acids |
| Retinol Acetate, Vit E, Biotin | Vitamins |
| D-Galactose, Pipecolic acid | Metabolic substrates |
| BSA Fraction V (IgG-free) | Carrier protein |
Applications
Naive Reprogramming
Convert primed hPSCs to the naive ground state. NaivaCore supports chemical reprogramming protocols validated for multiple hESC and iPSC lines.
Naive hPSC Expansion
Propagate naive hPSCs with maintained pluripotency markers (NANOG, KLF4, KLF17, TFCP2L1) and normal karyotype over extended passages.
Naivety Maintenance
Long-term maintenance of naive state without reversion to primed phenotype. Supports feeder-free and feeder-dependent culture formats.
iPSC Platform Development
Establish naive iPSC banks for longevity research, disease modelling, and epigenetic rejuvenation studies requiring ground-state starting material.
ATMP Upstream Research
Investigate naive pluripotency as a starting point for ATMP manufacturing process development, where naive cells may offer differentiation advantages.
Mechanistic Research
Study naive-specific signalling (JAK/STAT3, MEK/ERK, WNT) in a chemically defined, reproducible medium system with known inhibitor concentrations.
NaivaCore vs. Commercial Alternatives
| Parameter | NaivaCore SeamlessBio |
RSeT STEMCELL Technologies |
t2iLGö Academic formulation |
|---|---|---|---|
| Application | Reprogramming + Expansion + Maintenance | Reprogramming + Expansion | Maintenance (primarily) |
| Format | ✅ 3 separate components | Single formulation | DIY — no commercial kit |
| Inhibitor flexibility | ✅ Adjustable — separate SI vial | Fixed formulation | Self-prepared |
| Production region | ✅ EU (ISO-controlled) | Canada (North America) | — |
| EU documentation | ✅ CoA, CoO, stability data | Available | — |
| GMP pathway | ⚙️ In development | Not indicated | Not available |
| Custom batch sizes | ✅ Flexible | Standard catalogue sizes | — |
| Serum-free | ✅ | ✅ | ✅ |
Comparison based on publicly available product information. For research use only. RSeT is a trademark of STEMCELL Technologies Inc.
Scientific Background
Naive vs. Primed Pluripotency
Human pluripotent stem cells exist in two distinct states: the primed state (corresponding to the post-implantation epiblast) and the naive ground state (corresponding to the pre-implantation inner cell mass). Naive hPSCs display key characteristics including dome-shaped colony morphology, X-chromosome reactivation in female lines, global DNA hypomethylation, and enhanced developmental potential.
Conventional culture media (E8, mTeSR, Essential 8) maintain cells in the primed state. Accessing the naive ground state requires active suppression of primed-associated signalling (MEK/ERK, PKC, WNT) while activating naive-supporting pathways (JAK/STAT3 via LIF, Nodal via Activin A).
The HENSM Approach
NaivaCore is based on the Human Extended Naive Stem Cell Medium (HENSM) formulation developed at the Weizmann Institute of Science (Bayerl et al., Cell Stem Cell, 2021). HENSM demonstrated robust naive reprogramming across multiple hESC and iPSC lines and introduced CGP77675 (SRC inhibitor) as a key addition enabling stable naive maintenance — an advance over earlier t2iLGö and PXGL formulations.
Key Reference
Bayerl J et al. (2021). Principles of Self-Organization and Limits of Human Embryo Models. Cell, 185(15), 2778–2795.
Seltzer G & Hanna JH (2021). HENSM enables derivation and maintenance of human naive pluripotent stem cells. Cell Stem Cell, 28(6), 1016–1029.
Naive Markers Supported
- KLF4, KLF17
- TFCP2L1
- NANOG (naive-high)
- STELLA / DPPA3
- X-reactivation (female lines)
- Reduced LINE-1 methylation
Product Information
| NaivaCore Kit — Overview | |
|---|---|
| Product name | NaivaCore Naive Stem Cell Medium Kit |
| Components | NaivaCore-B | NaivaCore-SI ×100 | NaivaCore-NT ×50 |
| Species | Human (hPSC, hESC, hiPSC) |
| Culture format | Feeder-free and feeder-dependent |
| Serum-free | Yes |
| Available volumes | On request — flexible batch sizing |
| Storage (Basal) | 4°C, protect from light |
| Storage (SI, NT) | −20°C, single-use aliquots recommended |
| Shelf life | 6–12 months from production date (component-dependent) |
| Quality documentation | CoA, CoO, stability data, sterility certificate |
| Regulatory status | For research use only (RUO) |
| Production | EU — ISO-controlled conditions |
| Produced by | Seamless Bio GmbH (Switzerland) / SeamlessBio GmbH (Germany) |
Frequently Asked Questions
What cell lines is NaivaCore compatible with?
NaivaCore has been developed based on protocols validated across multiple hESC and iPSC lines. Compatibility across different genetic backgrounds should be confirmed by the user, as naive reprogramming efficiency can vary between lines.
Can I adjust the inhibitor concentrations in NaivaCore-SI?
Yes — this is a key advantage of the three-component format. Because NaivaCore-SI is supplied separately as a 100× concentrate, you can add it at varying dilutions to titrate individual pathway inhibition. This is not possible with single-formulation naive media.
What feeder system is recommended?
NaivaCore supports both feeder-free (Matrigel / Geltrex-coated plates) and MEF feeder-dependent culture. Geltrex Flex is included in NaivaCore-SI to support feeder-free naive colony attachment.
How does NaivaCore compare to t2iLGö or PXGL medium?
NaivaCore is based on the HENSM formulation which extends t2iLGö by adding SRC inhibition (CGP77675), which has been shown to improve naive maintenance stability. Unlike t2iLGö (which must be self-prepared from individual components), NaivaCore is available as a ready-to-use kit with consistent quality documentation.
Is batch reservation available?
Yes — SeamlessBio offers batch reservation at no prepayment. Contact info@seamlessbio.de to reserve a specific production lot for long-term experimental consistency.
Is NaivaCore available for ATMP or GMP applications?
NaivaCore is currently supplied for research use only. A GMP-compatible production pathway is under development. If you have ATMP development needs, contact us to discuss timeline, documentation requirements, and GMP transition planning.
Related Products
Request NaivaCore — Sample or Quote
Available in flexible batch sizes. EU documentation included. Batch reservation possible.
For research use only. Not for diagnostic or therapeutic use.
English 
Deutsch