BSA & Albumin/
Human Serum Albumin (HSA)
SeamlessBio — BSA & Albumin
Human Serum Albumin (HSA)
EU and US-origin human-derived albumin, purified from human serum/plasma — available as lyophilised powder and as sterile liquid solutions for xeno-free cell culture, ATMP manufacturing, biopharmaceutical formulation, and IVD applications.
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01
Human-Derived
Purified from human serum/plasma, eliminating xenogenic bovine protein exposure.
02
EU & US Origin
Collected from certified donor centres, fully documented and traceable per batch.
03
Viral Safety Tested
Full viral marker testing (HIV, HBsAg, HCV) per lot, with CoA provided.
04
No Minimum Order
From small research volumes to bulk supply, with batch reservation available.
Primary Product Range
HSA Powder — Lyophilised Human Serum Albumin
SeamlessBio Human Serum Albumin powder is the primary form of HSA — a lyophilised protein with the broadest application range across diagnostics, IVD reagent manufacturing, vaccine formulation, biopharmaceutical development, and research. Available in multiple grades from EU and US certified donor centres, with no minimum order quantity and batch reservation up to 36 months.
Standard Grade
HA1000
HSA Powder — Standard Grade
Lyophilised Human Serum Albumin from EU/US certified donor centres. High-purity freeze-dried albumin for use as a protein stabiliser, blocking agent, and carrier in diagnostic reagents, IVD kits, research buffers, and biological formulations.
- IVD and diagnostic reagent stabilisation
- Blocking agent in ELISA and immunoassays
- Vaccine formulation excipient
- Research buffer and media supplement
Pack sizes: 1 g · 10 g · 100 g · bulk on request
HA1020
HSA Powder — Fatty Acid Free
Lyophilised HSA with fatty acid content reduced to a minimum — for applications requiring a “clean” albumin protein without lipid background, such as binding studies, lipid transport research, and sensitive assay systems where endogenous fatty acids would interfere.
- Ligand binding and transport studies
- Lipid-sensitive assay systems
- Drug binding research (highly sensitive)
- Cell culture supplement in fatty acid studies
Pack sizes: 1 g · 10 g · 100 g · bulk on request
HA-1030
HSA Powder — Low B12 / Low Folate
Specialty HSA fraction with reduced Vitamin B12 and folate content — essential for nutritional research applications where endogenous vitamin carrier activity in standard albumin would create background interference. US origin (Golden West).
- Vitamin B12 and folate transport research
- Nutritional science and deficiency studies
- Metabolic assay development
- Reference material for diagnostic kit calibration
Pack sizes: 1 g · 10 g · 100 g · bulk on request
| Parameter | HA1000 — Standard | HA1020 — Fatty Acid Free | HA-1030 — Low B12/Folate |
|---|---|---|---|
| Form | Lyophilised powder | Lyophilised powder | Lyophilised powder |
| Purity | ≥96% albumin | ≥96% albumin | ≥96% albumin |
| Origin | EU / US certified donors | EU / US certified donors | US certified donors |
| Fatty acids | Standard | Reduced / free | Standard |
| Vitamin content | Standard | Standard | Low B12 / low folate |
| Endotoxin | Low (CoA data) | Low (CoA data) | Low (CoA data) |
| Documentation | CoA, CoO, SDS | CoA, CoO, SDS | CoA, CoO, SDS |
| Batch reservation | Up to 36 months | Up to 36 months | Up to 36 months |
Typical application areas for HSA powder
IVD & Diagnostics
Protein stabiliser in immunoassay reagents, ELISA kits, lateral flow strips, and point-of-care diagnostic systems.
Vaccine Formulation
Excipient and stabiliser in viral and bacterial vaccine formulations — standard in licensed vaccine products.
Biopharmaceutical R&D
Carrier protein and blocking agent in drug development screening, binding assays, and biological characterisation work.
Research & Cell Culture
Buffer supplement and media additive in research settings where lipid-free or vitamin-depleted albumin fractions are required.
HSA Liquid Solutions — Cell Culture & ATMP Grade
Cell Culture & ATMP Grade
HSA Liquid Solutions — ISO 13485, GMP-Adjacent Option
SeamlessBio HSA liquid solutions are cell culture grade — sterile, ready-to-use albumin solutions manufactured under ISO 13485-certified conditions with full EU-origin donor traceability, TSE/BSE freedom declaration, and GMP-adjacent documentation. Designed for use as ancillary materials in ATMP manufacturing, cell therapy process development, and biopharmaceutical production. Available in three concentrations, in glass vials and single-use bags.
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Grade note: HSA liquid solutions are manufactured under ISO 13485 and are suitable for R&D, process development, and GMP-adjacent Phase I/II manufacturing. For commercial GMP manufacturing requiring EP/USP pharmacopoeial compliance, contact our team to assess the appropriate grade for your regulatory strategy.
HSA 5% Solution 50 g/L
Isotonic albumin solution matching physiological plasma protein concentration. Standard choice for cell washing, density gradient separation, ATMP transfer buffers, and post-thaw DMSO dilution steps.
- T cell, NK cell & HSC washing steps
- Ficoll/Lymphoprep density gradient dilution buffer
- Post-thaw DMSO dilution and cell recovery
- Intermediate transfer buffers in ATMP manufacturing
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Industry Standard
20%
HSA 20% Solution 200 g/L
The international standard HSA concentration for cryopreservation and ATMP formulation. Combined with DMSO ≥99.9% as the extracellular protective component in CAR-T, NK cell, MSC, and HSC freezing media.
- Cryopreservation: 1–5% HSA final + 10% DMSO
- ATMP final formulation — cell suspension stabilisation
- Serum-free process medium supplement (0.5–2% final)
- Viral vector (AAV, lentivirus) stabilisation
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25%
HSA 25% Solution 250 g/L
Highest-concentration HSA liquid formulation — maximum protein protection at minimum added volume. For ATMP drug products with tight volume specifications and high-concentration AAV formulations. Direct alternative to Grifols Bio Supplies HSA 25%.
- ATMP final formulation with strict volume limits
- AAV gene therapy product stabilisation
- High-concentration cryopreservation protocols
- Grifols HSA 25% drop-in replacement (R&D / Phase I/II)
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| Parameter | HSA 5% | HSA 20% | HSA 25% |
|---|---|---|---|
| Concentration | 50 g/L | 200 g/L | 250 g/L |
| Purity | ≥96% albumin | ≥96% albumin | ≥96% albumin |
| Endotoxin | ≤5 EU/mL | ≤5 EU/mL | ≤5 EU/mL |
| Sterility | 0.2 µm filtered | 0.2 µm filtered | 0.2 µm filtered |
| Origin | EU certified donors | EU certified donors | EU certified donors |
| Quality standard | ISO 13485 | ISO 13485 | ISO 13485 |
| Packaging | Vial / single-use bag | Vial / single-use bag | Vial / single-use bag |
| Primary use | Washing & buffers | Cryo & formulation | Min-volume formulation |
Human-derived albumin for xeno-free applications
Human Serum Albumin (HSA) is purified from human serum or plasma, providing a human-matched alternative to bovine-derived BSA for applications where xenogenic protein exposure is a concern — particularly cell therapy, ATMP manufacturing, and biopharmaceutical formulation where animal-derived components require additional regulatory justification.
For a fully animal-free, donor-independent alternative, see our recombinant HSA (rHSA) range, available in Premium, Economy and CHO-expressed grades.
Typical applications
- ATMP and cell therapy manufacturing
- Xeno-free cell culture supplementation
- Biopharmaceutical formulation and excipient use
- Diagnostic reagent stabilisation
- Vaccine formulation
Product information
- Product type: Human Serum Albumin (powder & liquid)
- Origin: EU and/or US, certified donor centres
- Powder pack sizes: 1 g, 10 g, 100 g, bulk on request
- Liquid formats: glass vial (10 mL–1 L) or single-use bag (250 mL–5 L)
- Documentation: CoA, CoO, SDS, viral marker data
Available on request
- Batch-specific CoA for all grades
- Test samples (powder and liquid)
- TSE/BSE freedom declaration
- ATMP-relevant documentation package
- Bulk order options with batch reservation
Related albumin products
Recombinant HSA (rHSA)
HSA vs. rHSA — Comparison
BSA Low Endotoxin
All BSA & Albumin — Overview
Frequently Asked Questions — Human Serum Albumin (HSA)
What is the difference between HSA powder and HSA liquid solutions?
HSA powder (lyophilised) is the primary form of Human Serum Albumin — it has the broadest application range, including IVD reagent manufacturing, diagnostic kit stabilisation, vaccine formulation, and general research use. The powder form offers a longer shelf life and greater flexibility in reconstitution concentration. HSA liquid solutions (5%, 20%, 25%) are cell culture grade preparations manufactured under ISO 13485 — designed specifically for use as ancillary materials in cell therapy process steps such as cryopreservation, ATMP formulation, and cell washing. Liquid solutions are sterile and ready-to-use, avoiding the reconstitution step. SeamlessBio supplies both forms from EU and US certified donor centres.
What is the difference between HSA 5%, 20% and 25%?
The three concentrations serve different steps in cell therapy and biopharmaceutical manufacturing. HSA 5% (50 g/L) is an isotonic solution matching physiological plasma albumin levels — used for cell washing, PBMC isolation, and ATMP transfer buffers. HSA 20% (200 g/L) is the international industry standard for cryopreservation and ATMP final formulation, combined with DMSO in freezing media at 1–5% final concentration. HSA 25% (250 g/L) is the highest-concentration liquid formulation — relevant when total formulation volume is tightly constrained, such as in ATMP drug products with strict infusion volume specifications or high-concentration AAV gene therapy formulations.
What is HSA fatty acid free (HA1020) used for?
Fatty acid free HSA (HA1020) is a specialty albumin fraction from which endogenous fatty acids have been removed or reduced to a minimum. This makes it the correct choice for applications where the fatty acid binding and transport activity of standard albumin would create experimental interference — particularly ligand binding studies, lipid transport research, drug-albumin interaction assays, and cell culture experiments specifically studying lipid-related pathways. Standard HSA (HA1000) carries bound fatty acids at physiological levels and is not suitable for these applications. SeamlessBio offers fatty acid free HSA in lyophilised powder format in 1 g, 10 g, and 100 g pack sizes.
Is SeamlessBio HSA a suitable alternative to Grifols Bio Supplies HSA?
For research, process development, and GMP-adjacent Phase I/II manufacturing, yes. SeamlessBio HSA liquid solutions are manufactured under ISO 13485-certified conditions with full TSE/BSE freedom declaration and EU-origin donor traceability — matching the key quality parameters of Grifols Bio Supplies HSA for R&D and early-phase clinical applications. The practical advantages are availability and flexibility: no minimum order quantity, no sales pipeline, direct delivery from EU stock, and custom fill volumes in both glass vials and single-use bags. For commercial GMP manufacturing requiring EP/USP pharmacopoeial compliance, contact our team to assess the appropriate grade.
What is the standard HSA concentration in cryopreservation media for cell therapy?
The standard protocol for cryopreservation of cell therapy products (CAR-T, NK cells, MSCs, HSCs) combines 10% DMSO final with 1–5% HSA final, prepared from a 20% HSA stock solution, in a serum-free base medium (PBS or PlasmaLyte A). SeamlessBio supplies both HSA 20% and DMSO ≥99.9% from a single source with matched CoA and SDS documentation — simplifying supplier qualification and GMP-adjacent batch record management.
What is the difference between HSA (Human Serum Albumin) and rHSA (recombinant HSA)?
HSA is purified directly from human plasma collected at certified donor centres — a plasma-derived product with full viral safety testing per lot. rHSA (recombinant Human Serum Albumin) is produced in a non-human expression system (typically yeast or CHO cells), making it fully animal-free and donor-independent with no viral contamination risk. For most cell therapy and ATMP applications, plasma-derived HSA is the validated, widely-used standard. rHSA is preferred where complete freedom from human donor material is required — for example, fully xeno-free culture systems or processes where donor traceability creates regulatory complexity. SeamlessBio offers both plasma-derived HSA and recombinant HSA (rHSA) in multiple grades.
Need HSA for your application?
Contact our team for batch availability, documentation and delivery options — powder and liquid, all grades.
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