Separation Solution
Density gradient separation solution for PBMC isolation from whole blood or buffy coat, cell separation by buoyant density and related research workflows. Endotoxin-tested, sterile filtered, Certificate of Analysis per lot.
Product Details
| Parameter | Value |
|---|---|
| Density | 1.077 g/mL ± 0.001 at 20°C |
| Osmolality | ~280 mOsm/kg |
| pH | 7.2 – 7.4 |
| Sterility | Sterile filtered (0.2 µm) |
| Endotoxin | ≤ 1 EU/mL (LAL method) |
| Appearance | Clear, colourless to pale yellow solution |
| Packaging | 100 mL, 500 mL — other volumes on request |
| Storage | +2°C to +8°C, protect from light |
| Shelf life | 24 months from date of manufacture |
| Availability | On request |
Applications
PBMC Isolation
Isolation of peripheral blood mononuclear cells from human or animal whole blood and buffy coat by density gradient centrifugation at 400–800 × g.
T-Cell & NK-Cell Research
Preparation of lymphocyte-enriched fractions for T-cell activation assays, ELISPOT, mixed lymphocyte reactions and cytotoxicity assays.
Monocyte Isolation
Density-based enrichment of monocytes from PBMC fractions for macrophage differentiation, phagocytosis assays and innate immune research.
Bone Marrow Processing
Mononuclear cell isolation from bone marrow aspirates for haematopoietic stem cell research and MSC isolation protocols.
CAR-T Manufacturing
PBMC isolation as the first step in T-cell activation and transduction workflows for CAR-T cell manufacturing processes.
General Cell Density Separation
Separation of cell populations by buoyant density in any application requiring removal of dead cells, red blood cells or debris.
Frequently Asked Questions
What is separation solution used for?
Density gradient separation solution isolates PBMCs from whole blood or buffy coat by density gradient centrifugation. Cells layer at the interface based on buoyant density — mononuclear cells (1.070–1.077 g/mL) remain at the interface while erythrocytes and granulocytes pellet.
Is it endotoxin-tested?
Yes — sterile filtered (0.2 µm) and endotoxin-tested by LAL method (≤1 EU/mL). CoA provided per lot.
What volumes are available?
100 mL and 500 mL as standard. Other volumes on request — contact us with your requirement.
Related Products
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Application guide for PBMC isolation and immunological assays.
CAR-T Manufacturing
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Request Separation Solution
On request — contact us with your required volume and application.
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