Custom Cell Culture Media
Tailored cell culture media formulated for your cell line, process and regulatory requirements — from small research batches to commercial production volumes. Serum-free, chemically defined, xeno-free and animal-origin-free formulations available.
Start Your ProjectWhat We Develop
Serum-Free Media
Replace FBS with a defined, reproducible formulation. Retain performance while gaining regulatory compliance, simplified downstream processing and supply chain security.
Chemically Defined Media
All components identified at known concentrations. Maximum batch-to-batch consistency and full regulatory transparency for BLA, MAA and IMPD submissions.
Xeno-Free Media
Human-derived or recombinant components only — no non-human animal materials. Required for cell therapy products intended for clinical use and ATMP manufacturing.
Feed Supplements
Concentrated feed supplements matched to your basal medium. Optimised for fed-batch and perfusion processes to extend culture duration and improve titre.
Inhibitor Cocktails
Custom small molecule inhibitor supplements formulated to your exact concentrations — supplied as separate vials for protocol flexibility. Validated for stem cell, organoid and DMPK applications.
Powder Formulations
Dry powder media for extended shelf life, reduced cold-chain costs and flexible reconstitution at point of use. Available for most liquid formulations.
Cell Line Coverage
We develop custom media for all major mammalian cell lines and many specialised systems. If your cell line is not listed, contact us — we can source and develop for any system.
| Cell Line | Primary Application | Media Type |
|---|---|---|
| HEK293 / HEK293T / HEK293F | AAV, lentiviral vectors, recombinant protein | SF, CD, AOF — adherent & suspension |
| CHO-K1 / CHO-S / CHO DG44 | mAb, therapeutic protein, biosimilar | CD, protein-free — fed-batch & perfusion |
| VERO / MDCK / BHK-21 | Viral vaccine production | SF, CD — adherent & microcarrier |
| Hybridoma / Myeloma | mAb production for diagnostics | Low-protein SF |
| T-cells / NK cells / PBMCs | CAR-T, adoptive cell transfer | Xeno-free, GMP-ready |
| MSCs (bone marrow, adipose, cord) | Cell therapy, regenerative medicine | Xeno-free, hPL-supplemented |
| hPSC / iPSC (primed) | iPSC expansion, disease modelling | Defined, xeno-free |
| Naive hPSC | Ground state pluripotency, ATMP | NaivaCore (SI + NT components) |
| Organoids (intestinal, hepatic, brain) | Drug screening, disease modelling | OrganoMedium variants |
| Custom / novel cell lines | Any application | DoE-based development |
Development Process
Consultation
Define your cell line, application, target performance specs and regulatory requirements. We assess feasibility, component sourcing and timeline.
Formulation Design
Base medium selection, DoE-based nutrient screening, amino acid and lipid optimisation targeting your specific cell line and process parameters.
Testing & Validation
Growth kinetics, cell viability, productivity, product quality (glycosylation, aggregation) and stability assessment across multiple passages.
Scale-Up
Transfer from bench to bioreactor scale with process characterisation. Performance verification at target production scale.
GMP Production
Commercial manufacturing with full batch documentation, CoA, CoO, stability data. Ongoing supply with batch reservation available.
What We Offer
Flexible Batch Sizes
500 mL for early development through to 1000+ litres for commercial supply. No minimum order quantity for standard formulations.
Liquid & Powder
Ready-to-use liquid or dry powder for extended shelf life and reduced cold-chain cost. Custom packaging and aliquot sizes available.
Full Documentation
CoA, CoO, sterility certificate, endotoxin report, osmolality, pH — all included. DMF support available for regulatory submissions.
NDA-Protected Formulation
Your formulation remains confidential. We produce under signed NDA with full IP protection for client-proprietary recipes.
Batch Reservation
Reserve validated production lots for up to 6 weeks at no prepayment cost — ensuring lot continuity for long-running research or manufacturing programmes.
Technical Support
Dedicated project manager and scientific support throughout development, adaptation, scale-up and commercial supply phases.
Frequently Asked Questions
How long does custom media development take?
Simple modifications to an existing formulation take 2–4 weeks. Full custom development from scratch takes 8–16 weeks depending on cell line and complexity. GMP-grade production with complete documentation takes 3–6 months. A detailed project plan is provided at project start.
What volumes can you supply?
From 500 mL for initial research and development through to 1000+ litres for commercial production. Both liquid and dry powder formats are available. Multi-year supply agreements with volume commitments are available for commercial customers.
Can you reproduce a media formulation we already have?
Yes — we can reproduce existing formulations including licensed academic recipes (such as HENSM/NaivaCore), client-developed recipes, or modifications of commercial basal media. We handle all sourcing and QC under NDA.
Do you offer GMP-grade custom media production?
Yes. We produce under ISO 9001 and ISO 13485 conditions with GMP-compatible manufacturing capability. Full regulatory documentation is included — CoA, CoO, stability data, sterility and endotoxin certificates. DMF/ASMF support is available for regulatory submissions.
Ready-Made Media in Our Portfolio
Start Your Custom Media Project
Tell us your cell line, application and requirements. Our formulation team will respond within 48 hours.
+49 851 37932226 · info@seamlessbio.de
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