Description
Product Overview
CASY TT Cell Counter — distributed by SeamlessBio for OLS OMNI Life Science in DACH — uses Electrical Current Exclusion (ECE) technology to deliver accurate, reproducible cell counting and viability assessment without dye staining or subjective interpretation.
Unlike trypan blue exclusion, CASY TT measures individual cell volumes directly, enabling reliable differentiation of viable cells, dead cells, and debris — even in challenging cell types such as HEK293T, primary cells, or 3D spheroid-derived single-cell suspensions. For applications requiring precise seeding density control — viral vector production, ATMP manufacturing, or spheroid and 3D cell culture — CASY TT delivers the reproducibility that manual counting cannot.
CASY TT: Specifications
- Technology: Electrical Current Exclusion (ECE) — dye-free, label-free cell counting
- Model: CASY TT (benchtop, single-capillary)
- Measurement: Cell count, viability, cell volume distribution (size histogram)
- Cell size range: 3.5–120 µm (configurable)
- Throughput: <60 seconds per measurement
- Sample volume: 100 µL (in 10 mL CASY-ton dilution buffer)
- Output: Cell number/mL, viability (%), mean cell diameter, aggregation index
- Distribution: SeamlessBio — authorised DACH partner for OLS OMNI Life Science
Applications
- HEK293T seeding density control for AAV and lentiviral vector production — accurate titre prediction starts with accurate cell counts
- CHO, Vero, BHK cell counting in vaccine and recombinant protein manufacturing
- Primary cell and stem cell viability assessment — no staining artefacts
- Single-cell suspension QC after spheroid dissociation — see our spheroid culture applications
- Bioreactor seed train monitoring and scale-up density control
- GMP manufacturing QC where dye-free, audit-trail-capable counting is required
Available Models
| Model | Description |
|---|---|
| CASY TT | Benchtop cell counter — dye-free ECE technology, <60 s per measurement, GMP-compatible software with audit trail |
Key Benefits
Dye-Free ECE Technology
No trypan blue, no staining artefacts — direct cell volume measurement for objective, reproducible viability data.
GMP-Ready Software
Audit trail, user management, and 21 CFR Part 11-compatible data export for regulated manufacturing environments.
DACH Support via SeamlessBio
Authorised distributor for OLS OMNI Life Science in Germany, Austria and Switzerland — local demo, installation and application support.
Frequently Asked Questions
How does CASY TT differ from trypan blue exclusion counting?
Trypan blue relies on manual microscopic evaluation, is subject to operator variability, and systematically undercounts aggregating cell types such as HEK293T. CASY TT uses Electrical Current Exclusion: each cell displaces a volume of electrolyte as it passes through the capillary, and the resulting signal is measured as a precise cell volume. Viable cells, dead cells, and debris produce distinct size distributions that are separated algorithmically — no staining, no subjectivity, no operator-to-operator variability.
Why is accurate cell counting critical for HEK293T AAV production?
In transient transfection-based AAV manufacturing, seeding density at transfection directly determines DNA:cell ratio and, consequently, viral titre. A 20% error in cell count translates to a proportional loss in titre and process reproducibility. CASY TT eliminates counting uncertainty by measuring actual cell volume rather than relying on dye uptake, making it the preferred tool for AAV process development and GMP seed train control.
Can CASY TT count cells from dissociated 3D spheroids?
Yes — CASY TT is well suited for counting single-cell suspensions derived from spheroid dissociation, where cell aggregates and debris are common and trypan blue counting is unreliable. The ECE technology generates a full size-distribution histogram, allowing debris and incompletely dissociated aggregates to be gated out and only viable singlets counted. See our spheroid culture overview for application context.
Is CASY TT suitable for GMP or regulated manufacturing environments?
Yes — CASY TT includes GMP-compatible software with audit trail functionality, electronic signature support, and 21 CFR Part 11-compatible data export. It is used in regulated ATMP, vaccine, and recombinant protein manufacturing facilities. Qualification documentation (IQ/OQ support) is available on request through SeamlessBio.
Who distributes CASY TT in Germany, Austria and Switzerland?
SeamlessBio is the authorised DACH distributor for OLS OMNI Life Science and the CASY TT cell counter. We provide local demo units, application consultation, installation support, and ongoing customer service. Contact us for a quote, demo request, or application-specific guidance.
Request a Demo or Quote
48h response · DACH demo unit available · Local application support · Full documentation
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