Description
CelliMax® CHO FGb Medium is a chemically defined (CD) feed medium (amino acids only) for CHO cells, chemically defined feed containing only amino acids. Combined with CelliMax® CHO FGa or FGa plus, it supports high-density growth of CHO cell lines and enhances protein expression. Manufactured by our partner CelluPro and supplied in the EU by SeamlessBio.
Key features
- Chemically defined, amino acids only
- Cell lines: CHO-K1, CHO-DG44, CHO-S, CHO-DXB11 and GS-knockout CHO lines
- Applications: fed-batch culture in combination with CelliMax® CHO FGa or FGa plus
- Powder format for in-house preparation at any scale
- No L-glutamine included – add L-glutamine or a stabilised dipeptide according to your cell line and process
Specifications
| Manufacturer catalogue no. | CPDP064 |
|---|---|
| Product type | Feed medium (amino acids only) (powder) |
| Working concentration | 88.00 g/L |
| Target pH | pH 11.00–11.40 (alkaline concentrate) |
| Osmolality | 200–260 mOsmol/kg (measured at 5-fold dilution) |
| Density for weight-based make-up | 1.052 kg/L |
| Recommended minimum batch | 1 L |
| Sterile filtration | 0.2 or 0.22 µm, low-binding membrane (PVDF, PES or CA) |
| Storage | Powder sealed at 2–8 °C, protected from light; prepared medium at 2–8 °C, protected from light, use promptly |
| Shelf life | 24 months (unopened powder) |
| Intended use | Research use and manufacture of cell-culture-derived biological products; not for direct administration to humans or therapeutic use |
Fed-batch system
Use together with CelliMax® CHO FGa or FGa plus and a CelliMax® basal medium (MetaCHO or Versa).
Evaluation and documentation
Free evaluation samples, the manufacturer’s instructions for use and a fed-batch evaluation protocol are available on request. Prepare the medium with high-purity water (e.g. WFI) according to the instructions for use. See all serum-free CHO cell culture media.
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