Dimethyl Sulfoxide (DMSO) ≥99.9% | 100mL | sterile filtered

What is the standard DMSO concentration in cell therapy cryopreservation media?

The standard final DMSO concentration in cryopreservation media for cell therapy products (CAR-T, NK cells, MSCs, HSCs) is 10% v/v. This is combined with 1–5% HSA from a 20% stock solution and a compatible base medium (PBS, PlasmaLyte A, or serum-free medium). The 10% DMSO concentration has been validated across decades of clinical practice for haematopoietic stem cell transplantation and is the concentration referenced in the majority of ATMP product dossiers. Higher concentrations (up to 20%) are used for some specific applications such as sperm and oocyte cryopreservation, but are cytotoxic in standard mammalian cell culture. For cryopreservation of research cell lines, 90% FBS / 10% DMSO is a widely used alternative to serum-free freezing medium.

Is this DMSO suitable for clinical ATMP manufacturing (CAR-T, HSC)?

SeamlessBio DMSO ≥99.9% is USP-compliant and suitable for GMP-adjacent manufacturing processes, including use as a cryoprotectant excipient in Phase I and Phase II ATMP clinical trial batches. For licensed ATMPs where DMSO will be directly infused to patients as part of the final drug product (e.g. in cryopreserved CAR-T or HSC infusion bags), a medical device-registered DMSO such as WAK-Chemie CryoSure® is the standard regulatory approach in Europe and is listed in the European Pharmacopoeia. Contact our team to discuss which grade is appropriate for your regulatory strategy and phase of development.

Why does DMSO solidify, and does re-melting affect quality?

DMSO has an unusually high melting point of +18.5 °C for an organic solvent, meaning it solidifies at normal room temperature if the environment is cool. This is a physical property of the compound, not a quality defect. Solidified DMSO can be re-liquified by warming the sealed vial to room temperature (20–25 °C) — quality, purity, and sterility are not affected by this process as long as the vial remains sealed during re-melting. Never heat above 40 °C and never open a partially solidified vial, as this introduces moisture and risks contamination.

Can DMSO be ordered together with HSA 20% as a cryopreservation bundle?

Yes — and this is the recommended approach for cell therapy manufacturers. SeamlessBio supplies both DMSO ≥99.9% and HSA 20% Solution with matched batch documentation (CoA, CoO, SDS) from a single point of contact. Ordering both components together simplifies documentation management for GMP-adjacent processes, reduces supplier qualification burden, and ensures documentation consistency for ATMP batch records. Contact us with your required volumes and timeline for a combined quote.

What container materials are compatible with undiluted DMSO?

DMSO is a powerful solvent that degrades many common plastics. Compatible materials for storage and handling of undiluted DMSO include borosilicate glass (preferred), high-density polyethylene (HDPE), and fluoropolymers (PTFE, FEP). Incompatible materials include polystyrene, polypropylene, standard PVC tubing, and most rubber septa — DMSO will extract plasticisers and degrade the container wall, contaminating the product. SeamlessBio supplies DMSO in DMSO-resistant glass vials. When diluting for use, use glass or HDPE pipettes and containers; at concentrations below 20% v/v in aqueous solution, standard polypropylene labware is generally acceptable for short-term use.