Description
Product Overview
SeamlessBio FBS ES Cell Tested is fetal bovine serum specially screened and validated for use with embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and other sensitive pluripotent cell populations. Each batch is functionally tested to support undifferentiated growth, maintain typical pluripotent colony morphology, and confirm absence of cytotoxic or differentiation-inducing factors — enabling researchers to go directly to experiments without time-consuming in-house lot screening. Sourced from South American donor herds, sterile filtered at 0.1 µm, with full batch documentation. Learn more about what FBS is and how it supports stem cell culture.
Technical Specifications
| Parameter | Specification |
|---|---|
| Origin | South America — certified, traceable donor herds |
| Grade | ES Cell Tested — functionally validated for undifferentiated ESC/iPSC growth |
| Sterile filtration | 0.1 µm |
| Sterility | Tested — USP/EP method |
| Mycoplasma | Tested — negative |
| ES Cell test | Colony morphology, undifferentiated marker confirmation, cytotoxicity — lot-specific report available |
| Storage | −20 °C (cold chain required) |
| Documentation | CoA, BSE/TSE statement, CoO, ES Cell test report — batch-specific |
Applications
| Application | Description |
|---|---|
| hESC / mESC maintenance | Feeder-dependent culture of human and mouse embryonic stem cells — validated to support undifferentiated growth and self-renewal without triggering spontaneous differentiation |
| iPSC culture and reprogramming | Serum supplement for iPSC maintenance in feeder-dependent systems and for reprogramming protocols where defined serum-free media are not required |
| Stem cell expansion and cryopreservation | Routine expansion of pluripotent stocks and freeze medium component — validated lot ensures consistency across cryopreservation and recovery cycles |
| Differentiation assays | Defined baseline serum lot for directed differentiation protocols — single validated lot eliminates serum-driven variability across the differentiation time course |
Available Formats
| Format | Volume |
|---|---|
| Bottle | 100 mL |
| Bottle | 500 mL |
Key Benefits
Batch Reservation
Reserve validated ES Cell tested lots up to 36 months for reproducible long-term stem cell and iPSC workflows.
Full Documentation
CoA, BSE/TSE statement and ES Cell test report — from ISO 9001 and ISO 13485 certified partner facilities. Additional documents on request.
EU & Swiss Supply
Sourced from certified EU partner facilities — fast cold-chain delivery across EU, UK and Switzerland.
Frequently Asked Questions
What tests are performed to qualify FBS as ES Cell Tested?
ES Cell Tested FBS undergoes functional validation including colony morphology assessment (pluripotent colony morphology maintained), undifferentiated marker confirmation (alkaline phosphatase activity or Oct4/Sox2 staining), cytotoxicity testing, and standard QC parameters (sterility, mycoplasma, endotoxin, pH, osmolality). Only lots passing all criteria are released at ES Cell grade. A lot-specific ES Cell test report is available on request alongside the Certificate of Analysis.
Can ES Cell Tested FBS be used for iPSC culture?
Yes — FBS validated for ES Cell culture is equally suitable for iPSC maintenance in feeder-dependent systems. Both cell types share the same core pluripotency requirements: support for undifferentiated growth, absence of differentiation-inducing factors, and low cytotoxicity. The functional validation criteria used for ES Cell testing (colony morphology, Oct4/Sox2 expression, cytotoxicity) directly predict performance in iPSC culture. For feeder-free or xeno-free iPSC culture systems, a serum-free defined medium is typically preferred.
Why is lot-to-lot consistency especially critical for stem cell and iPSC culture?
Stem cells and iPSCs are uniquely sensitive to serum variability. Even small differences in growth factor concentrations, hormones, lipids, or trace contaminants between lots can trigger spontaneous differentiation, reduce cloning efficiency, or impair self-renewal. This makes serum lot screening one of the most time-consuming steps in stem cell laboratory setup. ES Cell Tested FBS eliminates this burden: each lot has already been functionally validated in ESC culture before release. Batch reservation for up to 36 months ensures a single validated lot can be used consistently across an entire research programme or clinical study, removing inter-lot variability as a confounding factor.
Is ES Cell Tested FBS suitable for feeder-free or xeno-free stem cell culture?
FBS is an animal-derived product and is not considered xeno-free. For feeder-free or xeno-free iPSC culture platforms — such as those required for GMP-grade cell therapy manufacturing or regulatory-compliant clinical applications — a defined, serum-free medium is the appropriate choice. FBS-based ES Cell Tested serum is best suited for established feeder-dependent culture systems where serum supplementation is part of the validated protocol and xeno-free status is not required. Contact our team to discuss serum-free alternatives for your specific application.
What documentation is included with FBS ES Cell Tested, and what is available on request?
Every order includes a Certificate of Analysis (CoA) with lot-specific QC data and ES Cell test results. Additional documentation available on request includes: BSE/TSE statement, Certificate of Origin (CoO), sterility certificate, mycoplasma test report, and endotoxin test data. All documentation is batch-specific and issued from ISO 9001 and ISO 13485 certified partner facilities.
Request a Quote or Sample
48h response · EU & Swiss Supply · Batch reservation available · Full documentation
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