Description
Product Overview
SeamlessBio Mouse Serum — Normal Mouse Serum (NMS), EU Origin is collected from healthy Mus musculus under controlled SPF (Specific Pathogen Free) housing conditions, sterile filtered at 0.2 µm, and batch-tested for purity and consistency. Available as individual or pooled serum. Supplied with full lot documentation for use in immunoassay development, monoclonal antibody production, and murine cross-reactivity studies.
Normal Mouse Serum is the species-matched blocking reagent of choice when murine primary or secondary antibodies are used in ELISA, Western blot, and immunofluorescence assays. It is also widely used as a negative control matrix in antibody species-specificity testing and as a supplement in hybridoma cultivation for monoclonal antibody screening. See the full portfolio on our Animal Sera overview page.
Technical Specifications
| Parameter | Specification |
|---|---|
| Species | Mus musculus (house mouse) |
| Designation | Normal Mouse Serum (NMS) — non-immunised donors, SPF housing |
| Origin | EU — controlled SPF housing facilities |
| Filtration | 0.2 µm sterile filtered |
| Mycoplasma | Negative (tested) |
| Storage | –20 °C long-term | +4 °C short-term |
| Shelf life | Min. 12 months from manufacture |
| Options | Individual or pooled serum; heat inactivated (56 °C, 30 min) on request; gamma-irradiated on request |
| Documentation | CoA, CoO, SDS; additional documents on request |
| Formats | 1 mL, 5 mL, 10 mL, 50 mL, 100 mL; bulk on request |
| Regulatory | Research Use Only (RUO) |
Applications
| Application | Protocol | Note |
|---|---|---|
| ELISA blocking | 1–5% NMS in PBS or assay buffer before antibody incubation | Reduces non-specific background signal when murine primary or secondary antibodies are used in ELISA — species-matched blocking prevents cross-reactivity with endogenous mouse immunoglobulin binding sites |
| Western blot / IFA | 1–5% NMS in blocking buffer (TBST or PBST) | Non-specific binding reduction in Western blot and immunofluorescence assays employing mouse-derived secondary antibodies |
| Hybridoma culture & mAb screening | NMS as culture supplement or assay matrix | Used in monoclonal antibody production workflows — NMS as a species-matched serum matrix in hybridoma screening ELISAs, or as supplement to support hybridoma cell growth |
| Cross-reactivity & species-specificity testing | NMS as negative control matrix | Antibody development and diagnostic kit validation — NMS confirms absence of cross-reactivity with murine proteins; reference matrix for assays targeting non-mouse analytes in a murine sample background |
Other Animal Sera Available
| Species | Typical Application | Availability |
|---|---|---|
| Rat serum | Immunoassay blocking, cross-reactivity control, rat cell culture | In stock |
| Mouse serum (NMS) | ELISA control, Western blot, hybridoma | In stock |
| Rabbit serum (NRS) | IHC, IFA, ELISA, antibody blocking | In stock |
| Sheep serum (NSS) | IHC blocking, complement studies, sheep-derived secondary | In stock |
| Goat serum (NGS) | IHC standard, IFA, ELISA, multiplex assays | In stock |
| Chicken serum | IgY assays, cross-reactivity, veterinary diagnostics | In stock |
| Pig serum | Preclinical models, porcine primary cell culture | In stock |
| Horse serum (DHS) | PC-12, hybridoma, insect cells, primary neurons | In stock |
| Guinea pig serum | Complement fixation test (CFT) | On request |
Available Formats
| Format | Size |
|---|---|
| Vial | 1 mL |
| Vial | 5 mL |
| Bottle | 10 mL |
| Bottle | 50 mL |
| Bottle | 100 mL |
| Bulk | On request |
Key Benefits
Batch Reservation
Reserve validated lots up to 36 months for reproducible long-term workflows.
Full Documentation
CoA, CoO and SDS — from ISO 9001 and ISO 13485 certified partner facilities. Additional documents on request.
EU & Swiss Supply
Sourced from certified EU partner facilities — fast cold-chain delivery across EU, UK and Switzerland.
Frequently Asked Questions
What is Normal Mouse Serum (NMS) used for in immunoassay development?
Normal Mouse Serum (NMS) is primarily used as a species-matched blocking reagent in ELISA, Western blot, and immunofluorescence assays where murine primary or secondary antibodies are employed. At 1–5% concentration in PBS or TBS-Tween buffer, it saturates non-specific binding sites and endogenous mouse immunoglobulin binding sites before antibody incubation, reducing background signal. NMS is also used as a negative control matrix to confirm species specificity when developing or validating antibodies — it establishes the baseline signal in a murine serum background without target-specific immunoreactivity.
Is Normal Mouse Serum (NMS) the same as pre-immune mouse serum?
Normal Mouse Serum (NMS) is collected from non-immunised, healthy donor mice and contains only background levels of antibodies — no deliberate immunisation has taken place. It corresponds to what is described in the scientific literature as NMS or pre-immune mouse serum: a species-matched, antigen-naïve serum matrix. SeamlessBio NMS is sourced from SPF-housed donors, ensuring a defined and consistent baseline immunoglobulin profile suitable for use as a blocking reagent and negative control.
Is pooled mouse serum available, and what are the differences from individual serum?
Yes — both individual (single-donor) and pooled Normal Mouse Serum are available. Pooled serum is a blend from multiple donor animals, which averages out individual variation in immunoglobulin levels and protein composition, producing a more homogeneous and reproducible reagent. Pooled NMS is preferred for ELISA blocking and negative control applications where lot-to-lot consistency is important. Individual serum may be appropriate for specific applications where donor traceability is required. Please specify individual or pooled when requesting a quote.
Is heat-inactivated or gamma-irradiated mouse serum available?
Yes — both heat inactivation (56 °C, 30 min) and gamma irradiation are available on request. Heat inactivation destroys complement proteins and is recommended for complement-sensitive immunoassay applications and cell-based protocols where complement activation must be avoided. Gamma-irradiated NMS is used when additional sterility assurance is required. Both treatments are documented in the lot Certificate of Analysis. Please specify the required treatment when requesting a quote.
What documentation is supplied with EU Origin Normal Mouse Serum?
Every order includes a Certificate of Analysis (CoA), Certificate of Origin (CoO), and Safety Data Sheet (SDS). Additional documentation — sterility and mycoplasma test protocols — is available on request. Products are handled at ISO 9001 and ISO 13485 certified partner facilities. Batch reservation for up to 36 months is available on request to ensure lot-to-lot reproducibility for long-term assay workflows.
Request a Quote or Sample
48h response · EU & Swiss Supply · Batch reservation available · Full documentation
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