0.1 µm vs 0.2 µm Sterile Filtration: Which Should You Use for Cell Culture and Diagnostics?
0.2 or 0.1 µm sterile filtration? What each retains (bacteria, mycoplasma), what neither removes, and how to filter serum and media for cell culture.
Heat Inactivation of FBS and Serum: Step-by-Step Protocol at 56 °C
Step-by-step heat inactivation of FBS and human serum at 56 °C for 30 min: thawing, reference bottle, timing, cooling, storage and common mistakes.
FBS Gamma Irradiation: How It Works, Which Dose and What It Achieves
How gamma irradiation of FBS works: frozen-state treatment, 25–45 kGy dose, dose mapping, virus and mycoplasma inactivation, documentation and limitations.
Papain Solution: Properties, Activity and Use Cases in Cell Culture
Papain explained: origin, cysteine protease activity, activation, inhibitors and protocols for cell detachment, tissue dissociation and Fab generation.
FBS Dialysis Protocol: How to Prepare Dialyzed Fetal Bovine Serum
Step-by-step protocol for dialyzing fetal bovine serum: membrane cut-off, buffer volume and changes, sterile filtration and QC for SILAC and metabolomics.
FBS Charcoal Stripping Protocol: How to Prepare Hormone-Depleted Serum
How to charcoal-strip FBS with dextran-coated charcoal: DCC preparation, incubation, removal, filtration and QC for hormone-depleted serum.
PBMC Isolation Protocol: Density Gradient Centrifugation with a Lymphocyte Separation Medium
Step-by-step PBMC isolation from whole blood with a 1.077 g/mL lymphocyte separation medium: dilution, layering, 400 × g spin, washing and troubleshooting.
Serum-Free MSC Medium for Basic Research — Defined, Reproducible
Home/ Blog/ MSC Medium for Basic Research Basic Research · MSC Biology MSC adhesion, migration, niche interaction, Wnt and TGF-b signalling — all are influenced by what is in the expansion medium. If that medium contains serum, you cannot fully separate your experimental signal from the background. Most MSC basic research labs expand cells in […]
MSC Medium for Drug Discovery — Reproducible Screening Models
Home/ Blog/ MSC Medium for Drug Discovery & CRO Screening Drug Discovery · CRO Your hit rate does not improve by running more compounds. It improves when the assay model stops being the variable. MSC expansion medium is where inter-run variability starts. MSCs are used across drug discovery as stromal components in co-culture models, as […]
MSC-Derived EVs Without Serum Contamination: The Medium Is the Starting Point
Home/ Blog/ MSC Medium for Exosome & EV Production Exosomes · Extracellular Vesicles FBS contains 1010–1012 EVs per mL. Every EV prep from serum-containing culture is co-isolated with bovine vesicles. If your downstream application requires MSC-specific EVs, the medium choice is not optional. MSC-derived exosomes and extracellular vesicles are under active development as therapeutic delivery […]