Recombinant Human Serum Albumin (rHSA)
Human Serum Albumin produced by genetic expression — fully xeno-free, no donor variability, identical sequence to native HSA. Three grades from cost-effective research use to clinical-stage CHO-expressed rHSA for ATMP manufacturing. All products on request.
rHSA by Grade
Three grades spanning from cost-effective research albumin to clinical-stage CHO-expressed rHSA. All fully xeno-free — no animal-derived components in the final product.
rHSA Economy
Yeast-expressed (Pichia pastoris) recombinant HSA at a cost-effective price point. Fully xeno-free for research and early process development. Drop-in replacement for BSA in serum-free media where xeno-free compliance is required.
- Expression: Yeast (Pichia pastoris)
- Purity: ≥96% (SDS-PAGE)
- Endotoxin: ≤5 EU/mg (LAL)
- Fully xeno-free — no animal components
- Research and process development use
rHSA Premium
High-purity yeast-expressed rHSA for cell therapy formulation and GMP-adjacent applications. Tighter endotoxin specification, higher purity and more extensive QC documentation. Standard for serum-free ATMP ancillary material files.
- Expression: Yeast (Pichia pastoris)
- Purity: ≥99% (SDS-PAGE)
- Endotoxin: ≤1 EU/mg (LAL)
- Cell therapy formulation and GMP-adjacent
- ATMP ancillary material documentation
rHSA CHO-Expressed
Highest purity rHSA produced in CHO mammalian expression system. For clinical-stage manufacturing where CHO origin documentation is required by regulatory agencies. Full GMP documentation package on request.
- Expression: CHO (mammalian cells)
- Purity: ≥99.5% (SDS-PAGE)
- Endotoxin: ≤0.5 EU/mg (LAL)
- Clinical-stage manufacturing
- Full GMP documentation on request
rHSA vs Plasma-Derived HSA — Quick Comparison
| Parameter | rHSA Economy | rHSA Premium | rHSA CHO | HSA Plasma-Derived |
|---|---|---|---|---|
| Source | Yeast | Yeast | CHO cells | Human plasma |
| Xeno-Free | ✅ Fully | ✅ Fully | ✅ Fully | Human-derived |
| Purity | ≥96% | ≥99% | ≥99.5% | Grade-dependent |
| Endotoxin | ≤5 EU/mg | ≤1 EU/mg | ≤0.5 EU/mg | ≤5 EU/mg |
| Lot variability | Very low | Very low | Minimal | Some donor variation |
| Best for | Research, process dev. | Cell therapy, GMP-adj. | Clinical manufacturing | IVD, pharma excipient |
Applications
Serum-Free Cell Culture
rHSA as xeno-free albumin supplement in serum-free and protein-free media formulations. Stabilises growth factors and provides carrier function without animal serum or plasma-derived components.
ATMP & Cell Therapy Formulation
rHSA Premium or CHO-expressed for ATMP ancillary material files where xeno-free documentation is required. CAR-T final formulation, MSC cryopreservation and iPSC media supplementation.
DMPK & Protein Binding
rHSA for plasma protein binding assays in drug metabolism studies. Defined fatty acid content for reproducible PPB results. Contact us for fatty acid-free rHSA specification.
Frequently Asked Questions
What is rHSA and how is it different from plasma-derived HSA?
rHSA is produced by genetic expression in yeast or CHO cells — no human plasma required. Fully xeno-free, no donor variability, no blood-derived contamination risk. Identical amino acid sequence to native human albumin.
Which grade should I choose?
Economy for research and process development. Premium for cell therapy formulation and GMP-adjacent applications. CHO-expressed for clinical-stage manufacturing where CHO origin documentation is required.
Is rHSA suitable for ATMP ancillary material files?
Yes — rHSA Premium and CHO-expressed come with ATMP ancillary material documentation on request. Fully xeno-free — compliant with EMA/410/01 guidance for xeno-free ATMP manufacturing.
When should I use HSA instead of rHSA?
Plasma-derived HSA is preferred for IVD calibrators and pharmaceutical excipient applications where pharmacopoeial (Ph. Eur./USP) compliance is required. rHSA is preferred for ATMP and cell therapy where xeno-free documentation is required.
Related Products
All rHSA Products — On Request
Contact us with your grade, volume and documentation requirements. Free test samples available. 48-hour response.
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