Description
CelliMax® Versa Medium is a chemically defined (CD) basal medium for CHO cells, free of animal-derived components and hydrolysates; contains no L-glutamine and no phenol red. Used with the CelliMax® feed media, it supports high-density growth of CHO cell lines and enhances protein expression. Manufactured by our partner CelluPro and supplied in the EU by SeamlessBio.
Key features
- Chemically defined, animal-component-free and hydrolysate-free
- Cell lines: CHO-K1, CHO-DG44, CHO-S and GS-knockout CHO lines
- Applications: cell thawing, passaging, fed-batch culture and cryopreservation
- Powder format for in-house preparation at any scale
- No L-glutamine included – add L-glutamine or a stabilised dipeptide according to your cell line and process
Specifications
| Manufacturer catalogue no. | CPRD098 |
|---|---|
| Product type | Basal medium (powder) |
| Working concentration | 25.45 g/L |
| Sodium bicarbonate | 2.20 g/L sodium bicarbonate |
| Target pH | pH 7.00–7.30 (final) |
| Osmolality | 300–350 mOsmol/kg |
| Density for weight-based make-up | 1.008 kg/L |
| Recommended minimum batch | 3 L |
| Sterile filtration | 0.2 or 0.22 µm, low-binding membrane (PVDF, PES or CA) |
| Storage | Powder sealed at 2–8 °C, protected from light; prepared medium at 2–8 °C, protected from light, use promptly |
| Shelf life | 24 months (unopened powder) |
| Intended use | Research use and manufacture of cell-culture-derived biological products; not for direct administration to humans or therapeutic use |
Fed-batch system
Combine with CelliMax® CHO FGa or FGa plus together with FGb for a complete fed-batch system.
Evaluation and documentation
Free evaluation samples, the manufacturer’s instructions for use and a fed-batch evaluation protocol are available on request. Prepare the medium with high-purity water (e.g. WFI) according to the instructions for use. See all serum-free CHO cell culture media.
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