Cell Biology & Imaging — Application Guides
Four expert guides covering the most common cell biology assay workflows — from scratch assay and cytotoxicity to 3D spheroid culture and automated live-cell monitoring. Each guide includes serum selection, protocol steps and product recommendations.
Cell biology assays are the foundation of preclinical drug discovery, cancer research and safety testing. The quality and consistency of your FBS, the endotoxin level, and the imaging approach directly determine the reproducibility and biological relevance of your data. These guides address the most common questions researchers face: which FBS grade to use, when to go serum-free, and how to automate monitoring for kinetic data.
Migration Scratch Assay — Protocol & Optimization Guide
Step-by-step wound healing assay protocol: serum strategy, confluency requirements, common pitfalls and automated gap closure quantification with live cell imaging.
Read guide →Cytotoxicity Assays — FBS & Serum Selection Guide
MTT, CellTiter-Glo, LDH and live/dead imaging: which FBS grade minimises serum interference, endotoxin effects on IC50, and when to use serum-free conditions.
Read guide →Spheroid & 3D Cell Culture — Protocol & FBS Guide
ULA plate methods, hanging drop, FBS concentration optimisation, drug screening in 3D tumour spheroids, and imaging approaches for size quantification.
Read guide →Live Cell Monitoring — zenCELL owl Incubator Microscope Guide
How to automate confluence tracking, scratch assay time-lapse and cytotoxicity monitoring inside your CO₂ incubator — label-free, 24 wells simultaneously.
Read guide →Recommended Products for Cell Biology Assays
Which FBS Grade for Which Assay?
A common source of variability in cell biology assays is inconsistent FBS grade selection. The table below summarises the correct choice for each workflow covered in this category:
| Assay | FBS Grade | Phase |
|---|---|---|
| Scratch assay — cell expansion | Standard | 10% |
| Scratch assay — migration phase | Standard | 1–2% |
| Cytotoxicity — standard screening | Standard | 10% |
| Cytotoxicity — NF-κB / cytokine drugs | Low Endotoxin <1 EU/mL | 5–10% |
| Spheroid formation | Standard | 2–10% |
| Live cell monitoring | Standard or Low Endotoxin | Per assay |
FBS Sample Set for Cell Biology Assays
Request test volumes of FBS Standard and FBS Low Endotoxin to validate on your specific cell line and assay platform before committing to a production lot.
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