HEK293 Transfection & Viral Vector Media
Chemically defined, animal-component-free transfection media for HEK293-based viral vector manufacturing — AAV, lentiviral, adenoviral and VLP production. Distributed in partnership with CelluPro (ISO 13485, EXCiPACT™). EU supply, no minimum order.
Viral Vector Optimised
Chemically defined transfection medium designed for HEK293-based AAV, LV, AdV and VLP production — high transient expression efficiency.
GMP-Grade Supply
ISO 13485, EXCiPACT™. Up to 250 kg GMP powder per batch, 200 L GMP liquid individually filled per run. Full batch documentation.
HEK293 / HEK293T / HEK293F
Compatible with adhesion, suspension and adapted HEK293 variants — lipofection and PEI-based transfection protocols supported.
ATMP & Regulatory Ready
Aligned with EMA ATMP Reg EC 1394/2007, FDA 21 CFR Part 1271, ICH Q5A/Q5D and EU GMP Annex 2 (2023) requirements.
Designed For: ATMP Manufacturers & Viral Vector CDMOs
SeamlessBio supplies HEK293 transfection and viral vector media to two primary customer groups: ATMP manufacturers (gene therapies, cell & gene therapy products, VLP-based vaccines) and viral vector CDMOs producing AAV, lentiviral and adenoviral vectors for clinical or commercial supply.
For ATMPs under EMA classification (EC 1394/2007) and biologics under FDA oversight, the supply chain documentation expectations are high — full traceability, lot-to-lot consistency, and GMP-certified manufacturing. CelluPro's ISO 13485 / EXCiPACT™ manufacturing standard meets these requirements from the raw material side.
SeamlessBio covers EU and UK supply, with batch reservation available for production campaigns.
Typical applications
- rAAV production — serotypes AAV2, AAV5, AAV8, AAV9, AAVrh10
- Lentiviral vector (LV) manufacturing for gene therapy
- Adenoviral vector (AdV) production — vaccine & gene delivery
- Virus-like particle (VLP) expression
- Transient transfection — triple plasmid AAV production
- Stable HEK293 cell line production systems
- ATMP upstream process development
- Clinical-phase and GMP CDMO supply
TransCHO Kit02 by CelluPro
Chemically defined, animal-component-free transfection medium kit for HEK293-based viral vector production. GMP powder and liquid formats with full batch documentation.
TransCHO Kit02
Chemically defined transfection medium optimised for HEK293 and HEK293T/F cell lines.
| Parameter | Specification |
|---|---|
| Product Name | TransCHO Kit02 |
| Manufacturer | CelluPro (ISO 13485, EXCiPACT™) |
| Cell Line Compatibility | HEK293, HEK293T, HEK293F (adhesion and suspension-adapted) |
| Formulation | Chemically defined, animal-component-free, serum-free |
| Transfection Method | Lipofection and PEI-based protocols |
| Applications | Transient transfection, stable line generation, viral vector production (AAV, LV, AdV, VLP) |
| Format Available | GMP powder (up to 250 kg/batch), GMP liquid (up to 200 L/run, individually filled) |
| Quality Standard | ISO 13485 · EXCiPACT™ |
| Documentation | CoA, SDS, full traceability records with every batch |
| Minimum Order | No minimum — free test samples available |
GMP Batch Capacities
Supported Vector Types
TransCHO Kit02 supports the major HEK293-based viral vector production platforms, all relevant for ATMP manufacturing and gene therapy supply.
Adeno-Associated Virus
HEK293 triple-plasmid transfection — AAV2, AAV5, AAV8, AAV9, AAVrh10. Compatible with BEF-free upstream processes.
- Transient triple-plasmid production
- Rep/Cap plasmid + GOI + helper
- Scales to bioreactor
Lentiviral Vector
HEK293T-based LV production for ex vivo and in vivo gene therapy applications. 3rd and 4th generation packaging systems.
- 3rd gen (transfer + packaging + envelope)
- 4th gen self-inactivating (SIN) vectors
- High titre process development
Adenoviral Vector
E1-deleted recombinant adenoviral vector production in HEK293 cells. Vaccine and gene delivery applications.
- E1-deleted Ad5 and variants
- Vaccine vector production
- Oncolytic adenovirus research
Virus-Like Particles
Non-replicating VLP expression in HEK293 and HEK293F cells. Vaccine, diagnostics and drug delivery applications.
- Enveloped and non-enveloped VLPs
- Suspension HEK293F compatible
- Diagnostic antigen production
Aligned With ATMP & Viral Vector Regulatory Requirements
CelluPro's manufacturing standard is designed to meet the raw material expectations of the most demanding regulatory frameworks in ATMP and biological manufacturing.
EMA ATMP Regulation
EC 1394/2007. Animal-component-free, chemically defined materials with full traceability and batch documentation — required for ATMP GMP compliance.
FDA 21 CFR Part 1271
HCT/P and cell therapy product upstream raw material requirements. ISO 13485-certified supply chain supports US IND and BLA submissions.
ICH Q5A / Q5D
Viral safety testing and cell substrate characterisation guidelines. Chemically defined, animal-free formulation removes hydrolysate and serum-based TSE/BSE risk.
EU GMP Annex 2 (2023)
Updated GMP Annex 2 for advanced therapy medicinal products. CelluPro ISO 13485 / EXCiPACT™ certification supports Annex 2 QMS integration for raw material suppliers.
CelluPro — ISO 13485 · EXCiPACT™
SeamlessBio distributes CelluPro's TransCHO Kit02 in the EU and UK. CelluPro develops and manufactures chemically defined cell culture media — including the TransCHO product line — using their proprietary PreciCHO™ ProPlatform.
ISO 13485 certification · EXCiPACT™ accreditation · EU, FDA and NMPA compliant. GMP powder up to 250 kg/batch · GMP liquid up to 200 L/run, individually filled.
EU supply chain active. EU-based warehousing in Germany in planning — contact SeamlessBio for current lead times.
Request a sample or quote for HEK293 transfection media
Free test samples available. No minimum order. 48-hour quote turnaround. Contact us for batch documentation, technical datasheets and custom lot sizes.
Contact UsFrequently Asked Questions
What is TransCHO Kit02 and which cell lines does it support?
TransCHO Kit02 is a chemically defined transfection medium kit by CelluPro, optimised for high-efficiency transient and stable transfection of HEK293, HEK293T, and HEK293F cell lines. It supports lipofection and PEI-based transfection protocols and is designed for viral vector production (AAV, lentiviral, adenoviral) and recombinant protein expression in HEK293-based systems.
Is HEK293 transfection media available in GMP grade?
Yes. CelluPro manufactures TransCHO Kit02 under ISO 13485-certified QMS with EXCiPACT™ accreditation. GMP powder batches up to 250 kg and GMP liquid runs up to 200 L individually filled are available with full batch documentation (CoA, SDS, traceability records) to support ATMP manufacturing and supplier qualification audits.
Which viral vector applications are supported?
TransCHO Kit02 supports AAV (adeno-associated virus) production including triple-plasmid transfection systems, lentiviral vector (LV) manufacturing with 3rd and 4th generation packaging, adenoviral vector (AdV) production, and VLP (virus-like particle) expression — all in HEK293-based systems. Compatible with adhesion and suspension-adapted HEK293F cells.
Is the media compliant with ATMP manufacturing requirements?
Yes. CelluPro's TransCHO Kit02 is manufactured under ISO 13485-certified QMS with EXCiPACT™ accreditation, aligned with EMA ATMP Regulation EC 1394/2007, FDA 21 CFR Part 1271, ICH Q5A/Q5D viral safety guidelines, and EU GMP Annex 2 (2023) for advanced therapy medicinal products.
Is there a minimum order quantity?
No minimum order quantity. Free test samples are available for product evaluation. Volume pricing and batch reservation for production campaigns are available on request — contact SeamlessBio.
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