Quick Definition
What is FBS Ultra Low IgG?: FBS Ultra Low IgG is fetal bovine serum processed to reduce bovine IgG to less than 5 µg/mL — the industry standard for diagnostic-grade monoclonal antibody production and high-sensitivity immunoassay development where trace bovine IgG contamination would cause assay interference or reduce antibody purity below acceptable limits.
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When is Ultra Low IgG required vs Low IgG FBS?
FBS Low IgG (<200 µg/mL) is sufficient for most research applications where final mAb purity after Protein A is >85%. FBS Ultra Low IgG (<5 µg/mL) is required when the mAb will be used as a diagnostic antibody where bovine IgG cross-reactivity creates false positives; when EU IVDR or FDA 510(k) submissions require demonstrated absence of bovine IgG interference; when the hybridoma produces <10 µg/mL mAb; or for therapeutic antibody research where xenogenic protein contamination must be minimised.
Key Facts
- FBS Ultra Low IgG: <5 µg/mL bovine IgG — confirmed by ELISA or RID per lot
- Standard FBS: 50–200 µg/mL bovine IgG — at least 10–40× more than Ultra Low IgG
- Equivalent to the industry-standard Ultra Low IgG specification at <5 µg/mL
- Used at 10–15% in hybridoma production medium — same concentration as standard FBS
- Single Protein A step after Ultra Low IgG culture typically yields >95% pure murine IgG
- Not required for fusion and cloning phases — use standard FBS until production scale
Further Reading
SeamlessBio · Passau, Germany · info@seamlessbio.de
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