For in-house DMPK transporter DDI assessment, you need ready-to-use ABC transporter vesicle kits that deliver reproducible IC50 data for IND-enabling regulatory submissions — without the lead time and cost of full CRO outsourcing for every compound. This page explains the options available in Europe and what makes Cell4Pharma kits, distributed by SeamlessBio, the practical choice for DACH-based DMPK teams.
Buy ABC transporter vesicle kits (HEK293-derived)
Request a quote for P-gp, BCRP, BSEP and MRP vesicle kits for in vitro DDI studies.
- P-gp (MDR1/ABCB1) vesicle kit
- BCRP (ABCG2) vesicle kit
- BSEP (ABCB11) vesicle kit
- MRP1 (ABCC1) vesicle kit
- MRP2 (ABCC2) vesicle kit
- MRP3 (ABCC3) vesicle kit
- MRP4 (ABCC4) vesicle kit
- MRP5 (ABCC5) vesicle kit
- MRP8 (ABCC11) vesicle kit
- P-gp vesicle kit with FluoPgp substrate
- Control vesicle kit (negative control)
What to look for in a DMPK vesicle kit supplier
| Criterion | Why it matters |
|---|---|
| Transporter coverage | A complete IND-enabling DDI package requires P-gp, BCRP, BSEP, and MRP2 at minimum. ICH M12 also recommends MRP3, MRP4. Buying from multiple suppliers for different transporters adds lot documentation complexity. |
| Regulatory acceptance | Vesicle kit data is only accepted in IND/BLA submissions if the kit is validated with characterised probe substrates, documented ATP/AMP ratio (assay window), and QC data. Off-the-shelf kits without validation documentation are not suitable for regulatory submissions. |
| Expression system | Sf9-based vesicles: high expression, good for P-gp and BSEP. HEK293-based: more physiologically relevant membrane, better for BCRP and MRP family. Both are accepted; matching the expression system to the transporter improves assay window. |
| ATP/AMP ratio — the critical QC parameter | The assay window (ATP-dependent vs AMP-dependent transport) must meet minimum specifications per lot. Kits without guaranteed ATP/AMP ratio produce variable IC50 results between lots — invalidating the dataset. |
| EU stock and delivery | US-based kit suppliers ship on fixed schedules (often 1–2× per week). For EU labs, this means 10–15 day lead times. EU-stocked kits ship in 2–4 business days — critical for IND timelines. |
Cell4Pharma vesicle kits — distributed by SeamlessBio in DACH
SeamlessBio is the exclusive DACH distributor for Cell4Pharma ABC Transporter Vesicle Kits — pre-prepared, QC-validated inside-out membrane vesicles for in-house IND-enabling DMPK transporter testing.
Available kits
- BSEP Vesicle Kit (ABCB11) — DILI risk assessment, EMA/ICH M12/PMDA recommended
- P-gp Vesicle Kit (ABCB1 / MDR1) — mandatory FDA/EMA, inhibitor IC50
- BCRP Vesicle Kit (ABCG2) — mandatory FDA/EMA, inhibitor IC50
- MRP1 Vesicle Kit (ABCC1)
- MRP2 Vesicle Kit (ABCC2) — hepatic efflux, cholestasis risk
- MRP3 Vesicle Kit (ABCC3)
- MRP4 Vesicle Kit (ABCC4)
- MRP5 Vesicle Kit (ABCC5)
- MRP8 Vesicle Kit (ABCC11)
- Control Vesicle Kit — non-transfected membrane for background subtraction
Kit specifications
- 100 reactions per kit
- Validated probe substrates, ATP regenerating system, assay buffer included
- Full assay protocol with step-by-step instructions
- Lot-specific QC data: ATP/AMP ratio, protein concentration, positive/negative control IC50
- Storage: −80°C; stable 12 months
- Expression systems: Sf9-based (P-gp, BSEP) and HEK293-based (MRP family, BCRP)
How Cell4Pharma compares to alternative DMPK kit suppliers
| Criterion | Cell4Pharma (via SeamlessBio) | Supplier A | Supplier B | Supplier C |
|---|---|---|---|---|
| BSEP kit available | ✅ | ✅ | ⚠️ Limited | ✅ |
| MRP1–MRP8 complete | ✅ MRP1,2,3,4,5,8 | ⚠️ Partial | ⚠️ Partial | ⚠️ Partial |
| HEK293-based MRP kits | ✅ | ⚠️ | ⚠️ | ⚠️ |
| Guaranteed ATP/AMP ratio per lot | ✅ | ⚠️ | ⚠️ | ✅ |
| EU stock — 2–4 day delivery | ✅ Passau, Germany | ✅ | ❌ US-based | ⚠️ Hungary |
| Control vesicle matched | ✅ | ✅ | ✅ | ✅ |
| Direct technical support | ✅ Direct access to Cell4Pharma scientists | ⚠️ Distributor chain | ⚠️ | ✅ |
| Swiss stock (no customs) | ✅ via seamlessbio.ch | ❌ | ❌ | ❌ |
Note: Competitor data based on publicly available information. Specifications change — verify directly with each supplier.
Which kit for which DDI study?
| DDI study type | Kit(s) required | Regulatory basis |
|---|---|---|
| P-gp inhibitor IC50 (high Papp compound) | P-gp Vesicle Kit + Control Kit | FDA 2020, EMA, ICH M12 — mandatory |
| BCRP inhibitor IC50 | BCRP Vesicle Kit + Control Kit | FDA 2020, EMA, ICH M12 — mandatory |
| BSEP DILI risk assessment | BSEP Vesicle Kit + Control Kit | ICH M12, EMA, PMDA — strongly recommended |
| Cholestasis risk (hepatic) | BSEP + MRP2 Vesicle Kits + Control Kit | EMA — recommended when hepatic signals present |
| Complete ABC efflux panel | P-gp + BCRP + BSEP + MRP2 + Control | Full IND-enabling package |
| Extended MRP screening | MRP1–MRP5, MRP8 + Control | Scientific rationale / comprehensive safety package |
Also available — ciPTEC renal transporter model
For OAT1, OAT3 and OCT2 renal DDI assessment under ICH M12, SeamlessBio Switzerland distributes ciPTEC — conditionally immortalised human proximal tubule cells that express all three mandatory renal uptake transporters in a single cell model. Unlike HK-2, ciPTEC expresses OAT1 and OAT3 endogenously — validated in >90 publications.
📞 +49 851 37932226
🇨🇭 Swiss customers: seamlessbio.ch — CHF billing, no import duties, ciPTEC also available
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